Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect

[EN] The gold standard for the treatment of peripheral nerve injuries, the autograft, presents several drawbacks, and engineered constructs are currently suitable only for short gaps or small diameter nerves. Here, we study a novel tissue-engineered multimodular nerve guidance conduit for the treatm...

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Autores: Gisbert-Roca, Fernando|||0000-0003-3937-5822, Martínez-Ramos, Cristina|||0000-0002-6540-4714, Monleón Pradas, Manuel|||0000-0001-6457-0414, Gil Santos, Luis, Mata Roig, Manuel, Milian Medina, Lara
Formato: artículo
Fecha de publicación:2022
País:España
Recursos:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/192910
Acesso em linha:https://riunet.upv.es/handle/10251/192910
Access Level:acceso abierto
Palavra-chave:Nerve guidance conduit
Hyaluronic acid
Polylactic acid
Aligned substrates
Schwann cells
Nerve regeneration
MAQUINAS Y MOTORES TERMICOS
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spelling Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve DefectGisbert-Roca, Fernando|||0000-0003-3937-5822Martínez-Ramos, Cristina|||0000-0002-6540-4714Monleón Pradas, Manuel|||0000-0001-6457-0414Gil Santos, LuisMata Roig, ManuelMilian Medina, LaraNerve guidance conduitHyaluronic acidPolylactic acidAligned substratesSchwann cellsNerve regenerationMAQUINAS Y MOTORES TERMICOS[EN] The gold standard for the treatment of peripheral nerve injuries, the autograft, presents several drawbacks, and engineered constructs are currently suitable only for short gaps or small diameter nerves. Here, we study a novel tissue-engineered multimodular nerve guidance conduit for the treatment of large nerve damages based in a polylactic acid (PLA) microfibrillar structure inserted inside several co-linear hyaluronic acid (HA) conduits. The highly aligned PLA microfibers provide a topographical cue that guides axonal growth, and the HA conduits play the role of an epineurium and retain the pre-seeded auxiliary cells. The multimodular design increases the flexibility of the device. Its performance for the regeneration of a critical-size (15 mm) rabbit sciatic nerve defect was studied and, after six months, very good nerve regeneration was observed. The multimodular approach contributed to a better vascularization through the micrometrical gaps between HA conduits, and the pre-seeded Schwann cells increased axonal growth. Six months after surgery, a cross-sectional available area occupied by myelinated nerve fibers above 65% at the central and distal portions was obtained when the multimodular device with pre-seeded Schwann cells was employed. The results validate the multi-module approach for the regeneration of large nerve defects and open new possibilities for surgical solutions in this field.This research was funded by the Spanish Government's State Research Agency (AEI) through projects DPI2015-72863-EXP and RTI2018-095872-B-C22/ERDF. Funding for open access charge: Universitat Politecnica de Valencia.MDPI AGDepartamento de Física AplicadaDepartamento de Termodinámica AplicadaCentro de Biomateriales e Ingeniería TisularEscuela Técnica Superior de Ingeniería IndustrialMinisterio de EducaciónAGENCIA ESTATAL DE INVESTIGACIONUniversitat Politècnica de ValènciaRepositorio Institucional de la Universitat Politècnica de València Riunet20222022-05-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/192910reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-095872-B-C22 NUEVO DISPOSITIVO BIOACTIVO PARA LA REGENERACION DE LESIONES DE LA MEDULA ESPINALMinisterio de Educación y Cultura http://dx.doi.org/10.13039/501100003176 FPU16%2F01833 FPU16%2F01833Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 DPI2015-72863-EXP NEUROCABLES MODULARES: MULTIPLICANDO CONEXIONES NEURALESopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1929102026-06-13T07:49:27Z
dc.title.none.fl_str_mv Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
title Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
spellingShingle Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
Gisbert-Roca, Fernando|||0000-0003-3937-5822
Nerve guidance conduit
Hyaluronic acid
Polylactic acid
Aligned substrates
Schwann cells
Nerve regeneration
MAQUINAS Y MOTORES TERMICOS
title_short Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
title_full Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
title_fullStr Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
title_full_unstemmed Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
title_sort Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect
dc.creator.none.fl_str_mv Gisbert-Roca, Fernando|||0000-0003-3937-5822
Martínez-Ramos, Cristina|||0000-0002-6540-4714
Monleón Pradas, Manuel|||0000-0001-6457-0414
Gil Santos, Luis
Mata Roig, Manuel
Milian Medina, Lara
author Gisbert-Roca, Fernando|||0000-0003-3937-5822
author_facet Gisbert-Roca, Fernando|||0000-0003-3937-5822
Martínez-Ramos, Cristina|||0000-0002-6540-4714
Monleón Pradas, Manuel|||0000-0001-6457-0414
Gil Santos, Luis
Mata Roig, Manuel
Milian Medina, Lara
author_role author
author2 Martínez-Ramos, Cristina|||0000-0002-6540-4714
Monleón Pradas, Manuel|||0000-0001-6457-0414
Gil Santos, Luis
Mata Roig, Manuel
Milian Medina, Lara
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Física Aplicada
Departamento de Termodinámica Aplicada
Centro de Biomateriales e Ingeniería Tisular
Escuela Técnica Superior de Ingeniería Industrial
Ministerio de Educación
AGENCIA ESTATAL DE INVESTIGACION
Universitat Politècnica de València
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Nerve guidance conduit
Hyaluronic acid
Polylactic acid
Aligned substrates
Schwann cells
Nerve regeneration
MAQUINAS Y MOTORES TERMICOS
topic Nerve guidance conduit
Hyaluronic acid
Polylactic acid
Aligned substrates
Schwann cells
Nerve regeneration
MAQUINAS Y MOTORES TERMICOS
description [EN] The gold standard for the treatment of peripheral nerve injuries, the autograft, presents several drawbacks, and engineered constructs are currently suitable only for short gaps or small diameter nerves. Here, we study a novel tissue-engineered multimodular nerve guidance conduit for the treatment of large nerve damages based in a polylactic acid (PLA) microfibrillar structure inserted inside several co-linear hyaluronic acid (HA) conduits. The highly aligned PLA microfibers provide a topographical cue that guides axonal growth, and the HA conduits play the role of an epineurium and retain the pre-seeded auxiliary cells. The multimodular design increases the flexibility of the device. Its performance for the regeneration of a critical-size (15 mm) rabbit sciatic nerve defect was studied and, after six months, very good nerve regeneration was observed. The multimodular approach contributed to a better vascularization through the micrometrical gaps between HA conduits, and the pre-seeded Schwann cells increased axonal growth. Six months after surgery, a cross-sectional available area occupied by myelinated nerve fibers above 65% at the central and distal portions was obtained when the multimodular device with pre-seeded Schwann cells was employed. The results validate the multi-module approach for the regeneration of large nerve defects and open new possibilities for surgical solutions in this field.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-05-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/192910
url https://riunet.upv.es/handle/10251/192910
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-095872-B-C22 NUEVO DISPOSITIVO BIOACTIVO PARA LA REGENERACION DE LESIONES DE LA MEDULA ESPINAL
Ministerio de Educación y Cultura http://dx.doi.org/10.13039/501100003176 FPU16%2F01833 FPU16%2F01833
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 DPI2015-72863-EXP NEUROCABLES MODULARES: MULTIPLICANDO CONEXIONES NEURALES
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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