Fast fabrication of reusable polyethersulfone microbial biosensors through biocompatible phase separation

In biosensors fabrication, entrapment in polymeric matrices allows efficient immobilization of the biorecognition elements without compromising their structure and activity. When considering living cells, the biocompatibility of both the matrix and the polymerization procedure are additional critica...

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Autores: Vigués Frantzen, Núria, Pujol Vila, F, Macanás de Benito, Jorge|||0000-0003-2346-3297, Muñoz Tapia, Maria, Muñoz Berbel, F. Xavier, Mas Gordi, Jordi
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/175628
Acceso en línea:https://hdl.handle.net/2117/175628
https://dx.doi.org/10.1016/j.talanta.2019.120192
Access Level:acceso abierto
Palabra clave:Biosensors
Biopolymers
Polymers
Thermoplastics
Bacterial immobilization
Phase separation
Polyethersulfone
Microbial biosensor
Respirometry
Toxicity assessment
Biopolímers
Polímers
Respirometria
Termoplàstics
Àrees temàtiques de la UPC::Enginyeria química
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spelling Fast fabrication of reusable polyethersulfone microbial biosensors through biocompatible phase separationVigués Frantzen, NúriaPujol Vila, FMacanás de Benito, Jorge|||0000-0003-2346-3297Muñoz Tapia, MariaMuñoz Berbel, F. XavierMas Gordi, JordiBiosensorsBiopolymersPolymersThermoplasticsBacterial immobilizationPhase separationPolyethersulfoneMicrobial biosensorRespirometryToxicity assessmentBiosensorsBiopolímersPolímersRespirometriaTermoplàsticsÀrees temàtiques de la UPC::Enginyeria químicaIn biosensors fabrication, entrapment in polymeric matrices allows efficient immobilization of the biorecognition elements without compromising their structure and activity. When considering living cells, the biocompatibility of both the matrix and the polymerization procedure are additional critical factors. Bio-polymeric gels (e.g. alginate) are biocompatible and polymerize under mild conditions, but they have poor stability. Most synthetic polymers (e.g. PVA), on the other hand, present improved stability at the expense of complex protocols involving chemical/physical treatments that decrease their biological compatibility. In an attempt to explore new solutions to this problem we have developed a procedure for the immobilization of bacterial cells in polyethersulfone (PES) using phase separation. The technology has been tested successfully in the construction of a bacterial biosensor for toxicity assessment. Biosensors were coated with a 300 μm bacteria-containing PES membrane, using non-solvent induced phase separation (membrane thickness≈300 μm). With this method, up to 2.3×106 cells were immobilized in the electrode surface with an entrapment efficiency of 8.2%, without compromising cell integrity or viability. Biosensing was performed electrochemically through ferricyanide respirometry, with metabolically-active entrapped bacteria reducing ferricyanide in the presence of glucose. PES biosensors showed good stability and reusability during dry frozen storage for up to 1 month. The analytical performance of the sensors was assessed carrying out a toxicity assay in which 3,5-dichlorophenol (DCP) was used as a model toxic compound. The biosensor provided a concentration-dependent response to DCP with half-maximal effective concentration (EC50) of 9.2 ppm, well in agreement with reported values. This entrapment methodology is susceptible of mass production and allows easy and repetitive production of robust and sensitive bacterial biosensors20192019-07-2920202020-01-24journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/175628https://dx.doi.org/10.1016/j.talanta.2019.120192reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1756282026-05-27T15:37:01Z
dc.title.none.fl_str_mv Fast fabrication of reusable polyethersulfone microbial biosensors through biocompatible phase separation
title Fast fabrication of reusable polyethersulfone microbial biosensors through biocompatible phase separation
spellingShingle Fast fabrication of reusable polyethersulfone microbial biosensors through biocompatible phase separation
Vigués Frantzen, Núria
Biosensors
Biopolymers
Polymers
Thermoplastics
Bacterial immobilization
Phase separation
Polyethersulfone
Microbial biosensor
Respirometry
Toxicity assessment
Biosensors
Biopolímers
Polímers
Respirometria
Termoplàstics
Àrees temàtiques de la UPC::Enginyeria química
title_short Fast fabrication of reusable polyethersulfone microbial biosensors through biocompatible phase separation
title_full Fast fabrication of reusable polyethersulfone microbial biosensors through biocompatible phase separation
title_fullStr Fast fabrication of reusable polyethersulfone microbial biosensors through biocompatible phase separation
title_full_unstemmed Fast fabrication of reusable polyethersulfone microbial biosensors through biocompatible phase separation
title_sort Fast fabrication of reusable polyethersulfone microbial biosensors through biocompatible phase separation
dc.creator.none.fl_str_mv Vigués Frantzen, Núria
Pujol Vila, F
Macanás de Benito, Jorge|||0000-0003-2346-3297
Muñoz Tapia, Maria
Muñoz Berbel, F. Xavier
Mas Gordi, Jordi
author Vigués Frantzen, Núria
author_facet Vigués Frantzen, Núria
Pujol Vila, F
Macanás de Benito, Jorge|||0000-0003-2346-3297
Muñoz Tapia, Maria
Muñoz Berbel, F. Xavier
Mas Gordi, Jordi
author_role author
author2 Pujol Vila, F
Macanás de Benito, Jorge|||0000-0003-2346-3297
Muñoz Tapia, Maria
Muñoz Berbel, F. Xavier
Mas Gordi, Jordi
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biosensors
Biopolymers
Polymers
Thermoplastics
Bacterial immobilization
Phase separation
Polyethersulfone
Microbial biosensor
Respirometry
Toxicity assessment
Biosensors
Biopolímers
Polímers
Respirometria
Termoplàstics
Àrees temàtiques de la UPC::Enginyeria química
topic Biosensors
Biopolymers
Polymers
Thermoplastics
Bacterial immobilization
Phase separation
Polyethersulfone
Microbial biosensor
Respirometry
Toxicity assessment
Biosensors
Biopolímers
Polímers
Respirometria
Termoplàstics
Àrees temàtiques de la UPC::Enginyeria química
description In biosensors fabrication, entrapment in polymeric matrices allows efficient immobilization of the biorecognition elements without compromising their structure and activity. When considering living cells, the biocompatibility of both the matrix and the polymerization procedure are additional critical factors. Bio-polymeric gels (e.g. alginate) are biocompatible and polymerize under mild conditions, but they have poor stability. Most synthetic polymers (e.g. PVA), on the other hand, present improved stability at the expense of complex protocols involving chemical/physical treatments that decrease their biological compatibility. In an attempt to explore new solutions to this problem we have developed a procedure for the immobilization of bacterial cells in polyethersulfone (PES) using phase separation. The technology has been tested successfully in the construction of a bacterial biosensor for toxicity assessment. Biosensors were coated with a 300 μm bacteria-containing PES membrane, using non-solvent induced phase separation (membrane thickness≈300 μm). With this method, up to 2.3×106 cells were immobilized in the electrode surface with an entrapment efficiency of 8.2%, without compromising cell integrity or viability. Biosensing was performed electrochemically through ferricyanide respirometry, with metabolically-active entrapped bacteria reducing ferricyanide in the presence of glucose. PES biosensors showed good stability and reusability during dry frozen storage for up to 1 month. The analytical performance of the sensors was assessed carrying out a toxicity assay in which 3,5-dichlorophenol (DCP) was used as a model toxic compound. The biosensor provided a concentration-dependent response to DCP with half-maximal effective concentration (EC50) of 9.2 ppm, well in agreement with reported values. This entrapment methodology is susceptible of mass production and allows easy and repetitive production of robust and sensitive bacterial biosensors
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-07-29
2020
2020-01-24
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/175628
https://dx.doi.org/10.1016/j.talanta.2019.120192
url https://hdl.handle.net/2117/175628
https://dx.doi.org/10.1016/j.talanta.2019.120192
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300724

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