A Two-Dimensional Human Minilung System (Model) for Respiratory Syncytial Virus Infections
Human respiratory syncytial virus (HRSV) is a major cause of serious pediatric respiratory diseases that lacks effective vaccine or specific therapeutics. Although our understanding about HRSV biology has dramatically increased during the last decades, the need for adequate models of HRSV infection...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Instituto de Salud Carlos III (ISCIII) |
| Repositorio: | Repisalud |
| Idioma: | inglés |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/7185 |
| Acceso en línea: | http://hdl.handle.net/20.500.12105/7185 |
| Access Level: | acceso abierto |
| Palabra clave: | Cell Differentiation Human Embryonic Stem Cells Humans Immunity, Innate Lung Organ Culture Techniques Respiratory Syncytial Virus Infections Respiratory Syncytial Virus, Human Virus Replication Models, Theoretical |
| Sumario: | Human respiratory syncytial virus (HRSV) is a major cause of serious pediatric respiratory diseases that lacks effective vaccine or specific therapeutics. Although our understanding about HRSV biology has dramatically increased during the last decades, the need for adequate models of HRSV infection is compelling. We have generated a two-dimensional minilung from human embryonic stem cells (hESCs). The differentiation protocol yielded at least six types of lung and airway cells, although it is biased toward the generation of distal cells. We show evidence of HRSV replication in lung cells, and the induction of innate and proinflammatory responses, thus supporting its use as a model for the study of HRSV-host interactions. |
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