The time dependent influence of curvature and topography of biomaterials in the behavior of corneal endothelial cells

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Government of Catalonia (2021 SGR 00565); the Spanish Ministry (Ramón y Cajal fellowship (RYC2018-025977-I), project PID2022-137962OB-I00 (MINECO...

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Detalhes bibliográficos
Autores: Bosch, Begoña M., Delgado, Luis M., Rodríguez-González, Raquel, Perez, Roman A.
Formato: artículo
Fecha de publicación:2024
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12328/4541
Acesso em linha:http://hdl.handle.net/20.500.12328/4541
https://dx.doi.org/10.3389/fbioe.2024.1454675
Access Level:acceso abierto
Palavra-chave:Còrnia
Cèl·lules endotelials corneals
Biomaterials
Enginyeria de teixits
Endoteli corneal
Córnea
Células endoteliales corneales
Biomateriales
Ingeniería de tejidos
Endotelio corneal
Cornea
Corneal endothelia cells
Tissue engineeering
Corneal endothelium
616.3
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spelling The time dependent influence of curvature and topography of biomaterials in the behavior of corneal endothelial cellsBosch, Begoña M.Delgado, Luis M.Rodríguez-González, RaquelPerez, Roman A.CòrniaCèl·lules endotelials cornealsBiomaterialsEnginyeria de teixitsEndoteli cornealCórneaCélulas endoteliales cornealesBiomaterialesIngeniería de tejidosEndotelio cornealCorneaCorneal endothelia cellsBiomaterialsTissue engineeeringCorneal endothelium616.3The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Government of Catalonia (2021 SGR 00565); the Spanish Ministry (Ramón y Cajal fellowship (RYC2018-025977-I), project PID2022-137962OB-I00 (MINECO/FEDER); and a predoctoral fellowship from Universitat Internacional de Catalunya (UIC).Among the different layers of the cornea, the corneal endothelium, which is composed of corneal endothelial cells (CEC), plays a key role in the maintenance of cornea transparency. Based on the donor shortages and the limitations associated with transplantation, in this work we have developed collagen hydrogels with different patterned structures on the surface to provide topographies in ranges similar to the natural environment that CEC sense. This aimed at stimulating cells to maintain a typical CEC phenotype and provide alternatives to corneal transplantation. In this sense, we have elaborated curved collagen hydrogels (concave and convex) with three different topographies (50, 200 and 300 µm grooves), with the help of 3D printed mold and replicating the mold with the collagen hydrogel, onto which CEC were cultured in order to analyze its behavior. Flat hydrogels were used as controls. Cell morphology, cell circularity and gene expression of ATP1A1 and ZO-1 genes were analyzed after 3 and 6 days. Results showed an effect of the curvature and the topography compared to flat collagen hydrogels, showing higher expression of ZO-1 and ATP1A1 in curved non-patterned hydrogels at day 3. The patterned hydrogels did not have such a significant effect on gene expression compared to flat hydrogels, showing stronger effect of the curvature compared to the topography. The effect was opposite at day 6, showing higher gene expression at days 6 on the patterned hydrogels, especially for the ZO-1 gene. The gene expression results were in accordance with the cell morphology observed at the different time points, showing circularities closer to hexagon like morphology at shorter time points. Overall, this platform can serve as a system to culture cell under proper environment to further be able to transplant a CEC monolayer or to allow transplantation of thin collagen layers with cultured CEC.info:eu-repo/semantics/publishedVersionFrontiers2024info:eu-repo/semantics/article13http://hdl.handle.net/20.500.12328/4541https://dx.doi.org/10.3389/fbioe.2024.1454675reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésFrontiers in Bioengineering and Biotechnology12info:eu-repo/grantAgreement/MINECO/PID2022-137962OB-I00/© 2025 Frontiers Media S.A.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12328/45412026-05-29T05:05:01Z
dc.title.none.fl_str_mv The time dependent influence of curvature and topography of biomaterials in the behavior of corneal endothelial cells
title The time dependent influence of curvature and topography of biomaterials in the behavior of corneal endothelial cells
spellingShingle The time dependent influence of curvature and topography of biomaterials in the behavior of corneal endothelial cells
Bosch, Begoña M.
Còrnia
Cèl·lules endotelials corneals
Biomaterials
Enginyeria de teixits
Endoteli corneal
Córnea
Células endoteliales corneales
Biomateriales
Ingeniería de tejidos
Endotelio corneal
Cornea
Corneal endothelia cells
Biomaterials
Tissue engineeering
Corneal endothelium
616.3
title_short The time dependent influence of curvature and topography of biomaterials in the behavior of corneal endothelial cells
title_full The time dependent influence of curvature and topography of biomaterials in the behavior of corneal endothelial cells
title_fullStr The time dependent influence of curvature and topography of biomaterials in the behavior of corneal endothelial cells
title_full_unstemmed The time dependent influence of curvature and topography of biomaterials in the behavior of corneal endothelial cells
title_sort The time dependent influence of curvature and topography of biomaterials in the behavior of corneal endothelial cells
dc.creator.none.fl_str_mv Bosch, Begoña M.
Delgado, Luis M.
Rodríguez-González, Raquel
Perez, Roman A.
author Bosch, Begoña M.
author_facet Bosch, Begoña M.
Delgado, Luis M.
Rodríguez-González, Raquel
Perez, Roman A.
author_role author
author2 Delgado, Luis M.
Rodríguez-González, Raquel
Perez, Roman A.
author2_role author
author
author
dc.subject.none.fl_str_mv Còrnia
Cèl·lules endotelials corneals
Biomaterials
Enginyeria de teixits
Endoteli corneal
Córnea
Células endoteliales corneales
Biomateriales
Ingeniería de tejidos
Endotelio corneal
Cornea
Corneal endothelia cells
Biomaterials
Tissue engineeering
Corneal endothelium
616.3
topic Còrnia
Cèl·lules endotelials corneals
Biomaterials
Enginyeria de teixits
Endoteli corneal
Córnea
Células endoteliales corneales
Biomateriales
Ingeniería de tejidos
Endotelio corneal
Cornea
Corneal endothelia cells
Biomaterials
Tissue engineeering
Corneal endothelium
616.3
description The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Government of Catalonia (2021 SGR 00565); the Spanish Ministry (Ramón y Cajal fellowship (RYC2018-025977-I), project PID2022-137962OB-I00 (MINECO/FEDER); and a predoctoral fellowship from Universitat Internacional de Catalunya (UIC).
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12328/4541
https://dx.doi.org/10.3389/fbioe.2024.1454675
url http://hdl.handle.net/20.500.12328/4541
https://dx.doi.org/10.3389/fbioe.2024.1454675
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Frontiers in Bioengineering and Biotechnology
12
info:eu-repo/grantAgreement/MINECO/PID2022-137962OB-I00/
dc.rights.none.fl_str_mv © 2025 Frontiers Media S.A.
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2025 Frontiers Media S.A.
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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