Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis

Available online 26 July 2018.

Bibliographic Details
Authors: Santos-Moriano, Paloma, Kidibule, Peter E, Alleyne, Elena, Ballesteros Olmo, Antonio, Heras, Ángeles, Fernández Lobato, María, Plou Gasca, Francisco José
Format: article
Status:Versión aceptada para publicación
Publication Date:2018
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/167998
Online Access:http://hdl.handle.net/10261/167998
Access Level:Open access
Keyword:Chitosan
Chitooligosaccharides
Chitosanase
Chitinases
Bacillus thuringiensis
Oligosaccharides
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spelling Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensisSantos-Moriano, PalomaKidibule, Peter EAlleyne, ElenaBallesteros Olmo, AntonioHeras, ÁngelesFernández Lobato, MaríaPlou Gasca, Francisco JoséChitosanChitooligosaccharidesChitosanaseChitinasesBacillus thuringiensisOligosaccharidesAvailable online 26 July 2018.Starting from a commercial preparation of Bacillus thuringiensis var. aizawai, widely employed as biopesticide in agriculture, we obtained a chitosanolytic activity by washing the cells with concentrated sodium acetate. The optimum pH and temperature values for this enzyme were 6.0 and 60 °C, respectively. The enzymatic extract efficiently hydrolyzed various chitosans with different deacetylation degrees (DD) into small oligosaccharides. Combining mass spectrometry (ESI-Q-TOF) and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), we observed the formation of fully deacetylated chitooligosaccharides [fdCOS, (GlcN)2-5] and, to a lesser extent, partially acetylated chitooligosaccharides [paCOS, GlcNAc-(GlcN)1-3]. A 10 g/L solution of chitosan with 600-800 kDa and DD ≥ 90% was fully converted into oligosaccharides in 55 h. Most of the products were fdCOS: 1.6 g/L of chitobiose, 1.7 g/L of chitotriose, 5 g/L of chitotetraose and 1.4 g/L of chitopentaose.This work was financed by grants from the Spanish Ministry of Economy and Competitiveness (grant number: BIO2013-48779-C4-1/-4 and BIO2016-76601-C3-1-/2-). We thank the support of COST-Action CM1303 on Systems Biocatalysts and of an institutional grant from the Fundación Ramón Areces to the Centro de Biologia Molecular Severo Ochoa. P. S-M. thanks the Spanish Ministry of Education for FPU Grant (grant number: FPU13/01185).Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Fundación Ramón ArecesMinisterio de Educación, Cultura y Deporte (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/167998reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2013-48779-C4-1-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2013-48779-C4-4-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-76601-C3-1-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-76601-C3-2-Rhttps://doi.org/10.1016/j.procbio.2018.07.017Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1679982026-05-22T06:33:51Z
dc.title.none.fl_str_mv Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis
title Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis
spellingShingle Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis
Santos-Moriano, Paloma
Chitosan
Chitooligosaccharides
Chitosanase
Chitinases
Bacillus thuringiensis
Oligosaccharides
title_short Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis
title_full Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis
title_fullStr Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis
title_full_unstemmed Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis
title_sort Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis
dc.creator.none.fl_str_mv Santos-Moriano, Paloma
Kidibule, Peter E
Alleyne, Elena
Ballesteros Olmo, Antonio
Heras, Ángeles
Fernández Lobato, María
Plou Gasca, Francisco José
author Santos-Moriano, Paloma
author_facet Santos-Moriano, Paloma
Kidibule, Peter E
Alleyne, Elena
Ballesteros Olmo, Antonio
Heras, Ángeles
Fernández Lobato, María
Plou Gasca, Francisco José
author_role author
author2 Kidibule, Peter E
Alleyne, Elena
Ballesteros Olmo, Antonio
Heras, Ángeles
Fernández Lobato, María
Plou Gasca, Francisco José
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Fundación Ramón Areces
Ministerio de Educación, Cultura y Deporte (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Chitosan
Chitooligosaccharides
Chitosanase
Chitinases
Bacillus thuringiensis
Oligosaccharides
topic Chitosan
Chitooligosaccharides
Chitosanase
Chitinases
Bacillus thuringiensis
Oligosaccharides
description Available online 26 July 2018.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/167998
url http://hdl.handle.net/10261/167998
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2013-48779-C4-1-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2013-48779-C4-4-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-76601-C3-1-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-76601-C3-2-R
https://doi.org/10.1016/j.procbio.2018.07.017
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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