Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure

This article belongs to the Section Molecular Plant Sciences.

Detalhes bibliográficos
Autores: Vázquez Prol, Francisco, Marquez-Molins, Joan, Rodrigo, Ismael, López-Gresa, Pilar, Bellés Albert, José-María, Gómez, Gustavo, Pallás Benet, Vicente, Lisón Párraga, Purificación
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/262317
Acesso em linha:http://hdl.handle.net/10261/262317
Access Level:Acceso aberto
Palavra-chave:Pospiviroidae
Stress
CEVd
Ribosome
TCDVd
Tomato
PSTVd
Eggplant
Agro-infiltration
Transcript
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spelling Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedureVázquez Prol, FranciscoMarquez-Molins, JoanRodrigo, IsmaelLópez-Gresa, PilarBellés Albert, José-MaríaGómez, GustavoPallás Benet, VicenteLisón Párraga, PurificaciónPospiviroidaeStressCEVdRibosomeTCDVdTomatoPSTVdEggplantAgro-infiltrationTranscriptThis article belongs to the Section Molecular Plant Sciences.Infectious viroid clones consist of dimeric cDNAs used to generate transcripts which mimic the longer-than-unit replication intermediates. These transcripts can be either generated in vitro or produced in vivo by agro-inoculation. We have designed a new plasmid, which allows both inoculation methods, and we have compared them by infecting Solanum lycopersicum and Solanum melongena with clones of Citrus exocortis virod (CEVd), Tomato chlorotic dwarf viroid (TCDVd), and Potato spindle tuber viroid (PSTVd). Our results showed more uniform and severe symptoms in agro-inoculated plants. Viroid accumulation and the proportion of circular and linear forms were different depending on the host and the inoculation method and did not correlate with the symptoms, which correlated with an increase in PR1 induction, accumulation of the defensive signal molecules salicylic (SA) and gentisic (GA) acids, and ribosomal stress in tomato plants. The alteration in ribosome biogenesis was evidenced by both the upregulation of the tomato ribosomal stress marker SlNAC082 and the impairment in 18S rRNA processing, pointing out ribosomal stress as a novel signature of the pathogenesis of nuclear-replicating viroids. In conclusion, this updated binary vector has turned out to be an efficient and reproducible method that will facilitate the studies of viroid–host interactions.This work was supported by the Spanish Ministry of Economy and Competitiveness (co-supported by FEDER) Grants BIO2017-88321-R (V.P.) and PID2019-104126RB-I00 (G.G.).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/262317reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-88321-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104126RB-I00https://doi.org/10.3390/ijms22126189Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2623172026-05-22T06:33:51Z
dc.title.none.fl_str_mv Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure
title Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure
spellingShingle Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure
Vázquez Prol, Francisco
Pospiviroidae
Stress
CEVd
Ribosome
TCDVd
Tomato
PSTVd
Eggplant
Agro-infiltration
Transcript
title_short Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure
title_full Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure
title_fullStr Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure
title_full_unstemmed Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure
title_sort Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure
dc.creator.none.fl_str_mv Vázquez Prol, Francisco
Marquez-Molins, Joan
Rodrigo, Ismael
López-Gresa, Pilar
Bellés Albert, José-María
Gómez, Gustavo
Pallás Benet, Vicente
Lisón Párraga, Purificación
author Vázquez Prol, Francisco
author_facet Vázquez Prol, Francisco
Marquez-Molins, Joan
Rodrigo, Ismael
López-Gresa, Pilar
Bellés Albert, José-María
Gómez, Gustavo
Pallás Benet, Vicente
Lisón Párraga, Purificación
author_role author
author2 Marquez-Molins, Joan
Rodrigo, Ismael
López-Gresa, Pilar
Bellés Albert, José-María
Gómez, Gustavo
Pallás Benet, Vicente
Lisón Párraga, Purificación
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pospiviroidae
Stress
CEVd
Ribosome
TCDVd
Tomato
PSTVd
Eggplant
Agro-infiltration
Transcript
topic Pospiviroidae
Stress
CEVd
Ribosome
TCDVd
Tomato
PSTVd
Eggplant
Agro-infiltration
Transcript
description This article belongs to the Section Molecular Plant Sciences.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/262317
url http://hdl.handle.net/10261/262317
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-88321-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104126RB-I00
https://doi.org/10.3390/ijms22126189
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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