Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine

The first amperometric immunosensor for the quantification of TGF-β1, a cytokine proposed as a biomarker for patients having or at risk for renal disease, is described in this work. The immunosensor design involves disposable devices using carboxylic acid-functionalized magnetic microparticles suppo...

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Detalles Bibliográficos
Autores: Sánchez Tirado, Esther, Martínez García, Gonzalo, González Cortés, Araceli, Yáñez-Sedeño Orive, Paloma, Pingarrón Carrazón, José Manuel
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/18770
Acceso en línea:https://hdl.handle.net/20.500.14352/18770
Access Level:acceso abierto
Palabra clave:543
Electrochemical immunosensor
Signal amplification
Transforming growth factor-β1 (TGF-β1)
Urine
Química analítica (Química)
2301 Química Analítica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/18770
network_acronym_str ES
network_name_str España
repository_id_str
spelling Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urineSánchez Tirado, EstherMartínez García, GonzaloGonzález Cortés, AraceliYáñez-Sedeño Orive, PalomaPingarrón Carrazón, José Manuel543Electrochemical immunosensorSignal amplificationTransforming growth factor-β1 (TGF-β1)UrineQuímica analítica (Química)2301 Química AnalíticaThe first amperometric immunosensor for the quantification of TGF-β1, a cytokine proposed as a biomarker for patients having or at risk for renal disease, is described in this work. The immunosensor design involves disposable devices using carboxylic acid-functionalized magnetic microparticles supported onto screen-printed carbon electrodes and covalent immobilization of the specific antibody for TGF-β1 using Mix&Go polymer. A sandwich-type immunoassay was performed using biotin-anti-TGF and conjugation with peroxidase-labeled streptavidin (poly-HRP-Strept) polymer. Amperometric measurements were carried out at 0.20 V by adding hydrogen peroxide solution onto the electrode surface in the presence of hydroquinone as the redox mediator. The calibration plot allowed a range of linearity extending between 15 and 3000 pg/mL TGF-β1 which is adequate for the determination of the cytokine in plasma and urine. The limit of detection, 10 pg/mL, is notably improved with respect to those obtained with ELISA kits. The usefulness of the immunosensor for the determination of low TGF-β1 concentrations in real samples was evaluated by analyzing spiked urine at different pg/mL concentration levels.ElsevierUniversidad Complutense de Madrid20172017-01-0120172017-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/18770reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available CTQ2015–70023-RComunidad de Madrid NANOAVANSENS S2013open accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttps://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/187702026-06-02T12:44:21Z
dc.title.none.fl_str_mv Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine
title Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine
spellingShingle Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine
Sánchez Tirado, Esther
543
Electrochemical immunosensor
Signal amplification
Transforming growth factor-β1 (TGF-β1)
Urine
Química analítica (Química)
2301 Química Analítica
title_short Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine
title_full Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine
title_fullStr Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine
title_full_unstemmed Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine
title_sort Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine
dc.creator.none.fl_str_mv Sánchez Tirado, Esther
Martínez García, Gonzalo
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author Sánchez Tirado, Esther
author_facet Sánchez Tirado, Esther
Martínez García, Gonzalo
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author_role author
author2 Martínez García, Gonzalo
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Electrochemical immunosensor
Signal amplification
Transforming growth factor-β1 (TGF-β1)
Urine
Química analítica (Química)
2301 Química Analítica
topic 543
Electrochemical immunosensor
Signal amplification
Transforming growth factor-β1 (TGF-β1)
Urine
Química analítica (Química)
2301 Química Analítica
description The first amperometric immunosensor for the quantification of TGF-β1, a cytokine proposed as a biomarker for patients having or at risk for renal disease, is described in this work. The immunosensor design involves disposable devices using carboxylic acid-functionalized magnetic microparticles supported onto screen-printed carbon electrodes and covalent immobilization of the specific antibody for TGF-β1 using Mix&Go polymer. A sandwich-type immunoassay was performed using biotin-anti-TGF and conjugation with peroxidase-labeled streptavidin (poly-HRP-Strept) polymer. Amperometric measurements were carried out at 0.20 V by adding hydrogen peroxide solution onto the electrode surface in the presence of hydroquinone as the redox mediator. The calibration plot allowed a range of linearity extending between 15 and 3000 pg/mL TGF-β1 which is adequate for the determination of the cytokine in plasma and urine. The limit of detection, 10 pg/mL, is notably improved with respect to those obtained with ELISA kits. The usefulness of the immunosensor for the determination of low TGF-β1 concentrations in real samples was evaluated by analyzing spiked urine at different pg/mL concentration levels.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01
2017
2017-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/18770
url https://hdl.handle.net/20.500.14352/18770
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available CTQ2015–70023-R
Comunidad de Madrid NANOAVANSENS S2013
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869408106380787712
score 15,300724

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