Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR
Seventy presumptive Listeria monocytogenes strains isolated from frozen vegetable products in a processing plant during a period of six months were characterized by serology, biochemical tests (API system), multiplex PCR, virulence characteristics and REP-PCR analysis. Most isolates belonged to 1/2a...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2011 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/63330 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/63330 |
| Access Level: | acceso abierto |
| Palabra clave: | Listeria monocytogenes REP-PCR Multiplex PCR Virulence Serology MICROBIOLOGIA |
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Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCRBallesteros Calabuig, LorenaCuesta Amat, GonzaloRodrigo, AlejandroTomás, DavidMoreno Trigos, Mª Yolanda|||0000-0003-3688-5157Hernández Pérez, Manuel|||0000-0003-1986-6501Ferrús Pérez, Mª AntoniaGarcía Hernández, Jorge|||0000-0003-1258-6128Listeria monocytogenesREP-PCRMultiplex PCRVirulenceSerologyMICROBIOLOGIASeventy presumptive Listeria monocytogenes strains isolated from frozen vegetable products in a processing plant during a period of six months were characterized by serology, biochemical tests (API system), multiplex PCR, virulence characteristics and REP-PCR analysis. Most isolates belonged to 1/2a (62 strains) and 1/2b (3 strains) serotypes, the most common isolated from foods. Amplification of virulence gene lmo2821 was positive in 51 of the PCR-confirmed L. monocytogenes strains. Automated REP-PCR of L. monocytogenes isolates yielded 17 different patterns, formed by 70 to 80 different bands ranging from 150 to 7000 bp. A total of three genetic groups were defined at 82% homology degree. At this level, serovars 4b and 1/2b strains were discriminated from the rest of strains. Two REP-PCR patterns were frequently found for isolates sharing the same serotype and biochemical profile. These isolates were obtained from different vegetables during the sampling period, what clearly suggests the persistence of some L. monocytogenes strains in the processing plant. While the multiplex PCR assay applied in this work provided an accurate and rapid method for species identification, REP-PCR was confirmed as a rapid and reliable method for L. monocytogenes subtyping, providing useful information for epidemiological or risk assessment studies, as well as for tracking surveys in food processing plants.This work was supported by grant AGL2008-05275-C03-02 from Ministerio de Ciencia e Innovacion, Spain.Wiley-BlackwellDepartamento de BiotecnologíaInstituto Universitario de Ingeniería del Agua y del Medio AmbienteCentro Avanzado de Microbiología AplicadaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalMinisterio de Ciencia e InnovaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20112011-05-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/63330reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 AGL2008-05275-C03-02 ANALISIS INTEGRADO DE RIESGOS EN AGUA Y ALIMENTOS PARA BACTERIAS PATOGENAS EMERGENTES POR METODOS MOLECULARES: DESINFECCION, SUPERVIVENCIA Y TRAZABILIDADopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/633302026-06-13T07:49:27Z |
| dc.title.none.fl_str_mv |
Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR |
| title |
Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR |
| spellingShingle |
Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR Ballesteros Calabuig, Lorena Listeria monocytogenes REP-PCR Multiplex PCR Virulence Serology MICROBIOLOGIA |
| title_short |
Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR |
| title_full |
Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR |
| title_fullStr |
Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR |
| title_full_unstemmed |
Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR |
| title_sort |
Persistence of Listeria monocytogenes strains in a frozen vegetables processing plant determined by serotyping and REP-PCR |
| dc.creator.none.fl_str_mv |
Ballesteros Calabuig, Lorena Cuesta Amat, Gonzalo Rodrigo, Alejandro Tomás, David Moreno Trigos, Mª Yolanda|||0000-0003-3688-5157 Hernández Pérez, Manuel|||0000-0003-1986-6501 Ferrús Pérez, Mª Antonia García Hernández, Jorge|||0000-0003-1258-6128 |
| author |
Ballesteros Calabuig, Lorena |
| author_facet |
Ballesteros Calabuig, Lorena Cuesta Amat, Gonzalo Rodrigo, Alejandro Tomás, David Moreno Trigos, Mª Yolanda|||0000-0003-3688-5157 Hernández Pérez, Manuel|||0000-0003-1986-6501 Ferrús Pérez, Mª Antonia García Hernández, Jorge|||0000-0003-1258-6128 |
| author_role |
author |
| author2 |
Cuesta Amat, Gonzalo Rodrigo, Alejandro Tomás, David Moreno Trigos, Mª Yolanda|||0000-0003-3688-5157 Hernández Pérez, Manuel|||0000-0003-1986-6501 Ferrús Pérez, Mª Antonia García Hernández, Jorge|||0000-0003-1258-6128 |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Biotecnología Instituto Universitario de Ingeniería del Agua y del Medio Ambiente Centro Avanzado de Microbiología Aplicada Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural Ministerio de Ciencia e Innovación Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
Listeria monocytogenes REP-PCR Multiplex PCR Virulence Serology MICROBIOLOGIA |
| topic |
Listeria monocytogenes REP-PCR Multiplex PCR Virulence Serology MICROBIOLOGIA |
| description |
Seventy presumptive Listeria monocytogenes strains isolated from frozen vegetable products in a processing plant during a period of six months were characterized by serology, biochemical tests (API system), multiplex PCR, virulence characteristics and REP-PCR analysis. Most isolates belonged to 1/2a (62 strains) and 1/2b (3 strains) serotypes, the most common isolated from foods. Amplification of virulence gene lmo2821 was positive in 51 of the PCR-confirmed L. monocytogenes strains. Automated REP-PCR of L. monocytogenes isolates yielded 17 different patterns, formed by 70 to 80 different bands ranging from 150 to 7000 bp. A total of three genetic groups were defined at 82% homology degree. At this level, serovars 4b and 1/2b strains were discriminated from the rest of strains. Two REP-PCR patterns were frequently found for isolates sharing the same serotype and biochemical profile. These isolates were obtained from different vegetables during the sampling period, what clearly suggests the persistence of some L. monocytogenes strains in the processing plant. While the multiplex PCR assay applied in this work provided an accurate and rapid method for species identification, REP-PCR was confirmed as a rapid and reliable method for L. monocytogenes subtyping, providing useful information for epidemiological or risk assessment studies, as well as for tracking surveys in food processing plants. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011 2011-05-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/63330 |
| url |
https://riunet.upv.es/handle/10251/63330 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 AGL2008-05275-C03-02 ANALISIS INTEGRADO DE RIESGOS EN AGUA Y ALIMENTOS PARA BACTERIAS PATOGENAS EMERGENTES POR METODOS MOLECULARES: DESINFECCION, SUPERVIVENCIA Y TRAZABILIDAD |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/ |
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openAccess |
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application/pdf application/pdf |
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Wiley-Blackwell |
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Wiley-Blackwell |
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reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
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Universitat Politècnica de València (UPV) |
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