Electrochemical Immunosensor Prototype for N-Terminal Natriuretic Peptide Detection in Human Saliva: Heart Failure Biomedical Application

NT-proBNP is the gold standard biomarker for early diagnostics of heart failure, disease prevention, and stratified and individualized patient care. In this work, we aim to develop a novel ultra-sensitive immunosensor for direct NT-proBNP detection in human artificial saliva (AS), which represents a...

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Autores: Baraket, Abdoullatif, Ghedir, El Kahina, Zine, Nadia, Jaffrezic-Renault, Nicole, Aarfane, Abdellatif, Nasrellah, Hamid, Belhora, Fouad, Bonet, Francisco Palacio, Bausells, Joan, Errachid, Abdelhamid
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/382503
Acceso en línea:http://hdl.handle.net/10261/382503
Access Level:acceso abierto
Palabra clave:Biosensor platform
N-terminal brain natriuretic peptide
Saliva analysis
Electrochemical impedance spectroscopy
Heart failure
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spelling Electrochemical Immunosensor Prototype for N-Terminal Natriuretic Peptide Detection in Human Saliva: Heart Failure Biomedical ApplicationBaraket, AbdoullatifGhedir, El KahinaZine, NadiaJaffrezic-Renault, NicoleAarfane, AbdellatifNasrellah, HamidBelhora, FouadBonet, Francisco PalacioBausells, JoanErrachid, AbdelhamidBiosensor platformN-terminal brain natriuretic peptideSaliva analysisElectrochemical impedance spectroscopyHeart failureNT-proBNP is the gold standard biomarker for early diagnostics of heart failure, disease prevention, and stratified and individualized patient care. In this work, we aim to develop a novel ultra-sensitive immunosensor for direct NT-proBNP detection in human artificial saliva (AS), which represents an intriguing biological matrix potentially rich in biomarkers. The immunosensor will enhance the sensitivity of detection, reduce measurement time, and enable the simultaneous detection of various biomarkers. The developed biosensor, based on gold working microelectrodes (WEs), was biofunctionalized using 4-carboxymethyl aryl diazonium (CMA) to immobilize anti-NT-proBNP antibodies. The deposition of CMA onto the gold surface of the microelectrodes was accomplished using cyclic voltammetry (CV). The binding between NT-proBNP antibodies and NT-proBNP antigens was tracked using electrochemical impedance spectroscopy (EIS) in conjunction with the standard addition method. A linear detection response within the range of 1–20 pg/mL for NT-proBNP detection in PBS and artificial saliva was demonstrated, with good selectivity in the presence of other potential interfering biomarkers (interleukin 6 (IL-6), interleukin 10 (IL-10), and interleukin 1 β (IL-1β)). The developed immunosensor shows great promise for rapid and accurate analysis in biomedical applications.The authors gratefully acknowledge the financial support provided by Campus France through the French-German scientific program (PHC PROCOPE/49540WG), by the European project GREENSMARTMED, and by the Moroccan national project AVENIR-ESEF from the High School of Education and Training El Jadida, Chouaïb Doukkali University, El Jadida, Morocco.Peer reviewedMultidisciplinary Digital Publishing InstituteCampus FranceEuropean CommissionUniversité Chouaïb DoukkaliConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/382503reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/chemosensors13020066https://doi.org/10.3390/chemosensors13020066Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3825032026-05-22T06:33:51Z
dc.title.none.fl_str_mv Electrochemical Immunosensor Prototype for N-Terminal Natriuretic Peptide Detection in Human Saliva: Heart Failure Biomedical Application
title Electrochemical Immunosensor Prototype for N-Terminal Natriuretic Peptide Detection in Human Saliva: Heart Failure Biomedical Application
spellingShingle Electrochemical Immunosensor Prototype for N-Terminal Natriuretic Peptide Detection in Human Saliva: Heart Failure Biomedical Application
Baraket, Abdoullatif
Biosensor platform
N-terminal brain natriuretic peptide
Saliva analysis
Electrochemical impedance spectroscopy
Heart failure
title_short Electrochemical Immunosensor Prototype for N-Terminal Natriuretic Peptide Detection in Human Saliva: Heart Failure Biomedical Application
title_full Electrochemical Immunosensor Prototype for N-Terminal Natriuretic Peptide Detection in Human Saliva: Heart Failure Biomedical Application
title_fullStr Electrochemical Immunosensor Prototype for N-Terminal Natriuretic Peptide Detection in Human Saliva: Heart Failure Biomedical Application
title_full_unstemmed Electrochemical Immunosensor Prototype for N-Terminal Natriuretic Peptide Detection in Human Saliva: Heart Failure Biomedical Application
title_sort Electrochemical Immunosensor Prototype for N-Terminal Natriuretic Peptide Detection in Human Saliva: Heart Failure Biomedical Application
dc.creator.none.fl_str_mv Baraket, Abdoullatif
Ghedir, El Kahina
Zine, Nadia
Jaffrezic-Renault, Nicole
Aarfane, Abdellatif
Nasrellah, Hamid
Belhora, Fouad
Bonet, Francisco Palacio
Bausells, Joan
Errachid, Abdelhamid
author Baraket, Abdoullatif
author_facet Baraket, Abdoullatif
Ghedir, El Kahina
Zine, Nadia
Jaffrezic-Renault, Nicole
Aarfane, Abdellatif
Nasrellah, Hamid
Belhora, Fouad
Bonet, Francisco Palacio
Bausells, Joan
Errachid, Abdelhamid
author_role author
author2 Ghedir, El Kahina
Zine, Nadia
Jaffrezic-Renault, Nicole
Aarfane, Abdellatif
Nasrellah, Hamid
Belhora, Fouad
Bonet, Francisco Palacio
Bausells, Joan
Errachid, Abdelhamid
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Campus France
European Commission
Université Chouaïb Doukkali
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biosensor platform
N-terminal brain natriuretic peptide
Saliva analysis
Electrochemical impedance spectroscopy
Heart failure
topic Biosensor platform
N-terminal brain natriuretic peptide
Saliva analysis
Electrochemical impedance spectroscopy
Heart failure
description NT-proBNP is the gold standard biomarker for early diagnostics of heart failure, disease prevention, and stratified and individualized patient care. In this work, we aim to develop a novel ultra-sensitive immunosensor for direct NT-proBNP detection in human artificial saliva (AS), which represents an intriguing biological matrix potentially rich in biomarkers. The immunosensor will enhance the sensitivity of detection, reduce measurement time, and enable the simultaneous detection of various biomarkers. The developed biosensor, based on gold working microelectrodes (WEs), was biofunctionalized using 4-carboxymethyl aryl diazonium (CMA) to immobilize anti-NT-proBNP antibodies. The deposition of CMA onto the gold surface of the microelectrodes was accomplished using cyclic voltammetry (CV). The binding between NT-proBNP antibodies and NT-proBNP antigens was tracked using electrochemical impedance spectroscopy (EIS) in conjunction with the standard addition method. A linear detection response within the range of 1–20 pg/mL for NT-proBNP detection in PBS and artificial saliva was demonstrated, with good selectivity in the presence of other potential interfering biomarkers (interleukin 6 (IL-6), interleukin 10 (IL-10), and interleukin 1 β (IL-1β)). The developed immunosensor shows great promise for rapid and accurate analysis in biomedical applications.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/382503
url http://hdl.handle.net/10261/382503
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/chemosensors13020066
https://doi.org/10.3390/chemosensors13020066
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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