Label-free aptasensor for lysozyme detection using electrochemical impedance spectroscopy
This research develops a label-free aptamer biosensor (aptasensor) based on graphite-epoxy composite electrodes (GECs) for the detection of lysozyme protein using Electrochemical Impedance Spectroscopy (EIS) technique. The chosen immobilization technique was based on covalent bonding using carbodiim...
| Autores: | , |
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| Formato: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Recursos: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:199392 |
| Acesso em linha: | https://ddd.uab.cat/record/199392 https://dx.doi.org/urn:doi:10.3390/s18020354 |
| Access Level: | acceso abierto |
| Palavra-chave: | Aptamer Biosensor Electrochemical grafting Electrochemical impedance spectroscopy |
| Resumo: | This research develops a label-free aptamer biosensor (aptasensor) based on graphite-epoxy composite electrodes (GECs) for the detection of lysozyme protein using Electrochemical Impedance Spectroscopy (EIS) technique. The chosen immobilization technique was based on covalent bonding using carbodiimide chemistry; for this purpose, carboxylic moieties were first generated on the graphite by electrochemical grafting. The detection was performed using [Fe(CN)]/[Fe(CN)] as redox probe. After recording the frequency response, values were fitted to its electric model using the principle of equivalent circuits. The aptasensor showed a linear response up to 5 µMfor lysozyme and a limit of detection of 1.67 µM. The sensitivity of the established method was 0.090 µM in relative charge transfer resistance values. The interference response by main proteins, such as bovine serum albumin and cytochrome c, has been also characterized. To finally verify the performance of the developed aptasensor, it was applied to wine analysis. |
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