Immuno-battery: A single use self-powered immunosensor for REASSURED diagnostics

In this work, we present a novel self-powered approach totally independent from any external energy source. We have developed a self-powered paper-based immunosensor that generates energy in the presence of the biomarker in the sample. In particular, the device - which has been labeled as Immuno-Bat...

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Detalles Bibliográficos
Autores: Galyamin, Dmitry, Liébana, Susana, Esquivel Bojórquez, Juan Pablo, Sabaté Vizcarra, Neus
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/350959
Acceso en línea:http://hdl.handle.net/10261/350959
https://api.elsevier.com/content/abstract/scopus_id/85141282193
Access Level:acceso abierto
Palabra clave:Diagnostics
Immunoassay
REASSURED
Self-powered sensor
ePAD
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spelling Immuno-battery: A single use self-powered immunosensor for REASSURED diagnosticsGalyamin, DmitryLiébana, SusanaEsquivel Bojórquez, Juan PabloSabaté Vizcarra, NeusDiagnosticsImmunoassayREASSUREDSelf-powered sensorePADIn this work, we present a novel self-powered approach totally independent from any external energy source. We have developed a self-powered paper-based immunosensor that generates energy in the presence of the biomarker in the sample. In particular, the device - which has been labeled as Immuno-Battery - makes use of magnesium as anode and the widely employed HRP-labeled antibody as cathodic catalyst to detect C-reactive protein (CRP) presence in artificial samples. Feasibility of self-powered sensing is proved by submitting the immuno-battery to a resistive load. In this regime, the sensor provides operation voltages above 1.55 V and maximum power densities from 40 to 571 μW cm-2 that allow for future implementation of an electronic readout circuit. We have demonstrated that sensitivity of the system is not compromised by the self-powered mode operation, as the LOD value delivered by our battery (20 ± 2 ng mL-1) is compliant with LOD values reported for protein detection in paper-based electrochemical immunoassays with chronoamperometric methods. Moreover, as a case study, a LOD of 269 ± 39 ng mL-1 is obtained for CRP detection, in accordance with available commercial high-sensitivity CRP detection kits. This proof-of-concept opens the path towards the development of digital diagnostic devices in a sustainable and affordable manner.Peer reviewedElsevierEuropean Commission0000-0002-4301-7027Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/350959https://api.elsevier.com/content/abstract/scopus_id/85141282193reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/648518Biosensors & bioelectronicshttps://www.sciencedirect.com/science/article/pii/S0956566322009083Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3509592026-05-22T06:33:51Z
dc.title.none.fl_str_mv Immuno-battery: A single use self-powered immunosensor for REASSURED diagnostics
title Immuno-battery: A single use self-powered immunosensor for REASSURED diagnostics
spellingShingle Immuno-battery: A single use self-powered immunosensor for REASSURED diagnostics
Galyamin, Dmitry
Diagnostics
Immunoassay
REASSURED
Self-powered sensor
ePAD
title_short Immuno-battery: A single use self-powered immunosensor for REASSURED diagnostics
title_full Immuno-battery: A single use self-powered immunosensor for REASSURED diagnostics
title_fullStr Immuno-battery: A single use self-powered immunosensor for REASSURED diagnostics
title_full_unstemmed Immuno-battery: A single use self-powered immunosensor for REASSURED diagnostics
title_sort Immuno-battery: A single use self-powered immunosensor for REASSURED diagnostics
dc.creator.none.fl_str_mv Galyamin, Dmitry
Liébana, Susana
Esquivel Bojórquez, Juan Pablo
Sabaté Vizcarra, Neus
author Galyamin, Dmitry
author_facet Galyamin, Dmitry
Liébana, Susana
Esquivel Bojórquez, Juan Pablo
Sabaté Vizcarra, Neus
author_role author
author2 Liébana, Susana
Esquivel Bojórquez, Juan Pablo
Sabaté Vizcarra, Neus
author2_role author
author
author
dc.contributor.none.fl_str_mv European Commission
0000-0002-4301-7027
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Diagnostics
Immunoassay
REASSURED
Self-powered sensor
ePAD
topic Diagnostics
Immunoassay
REASSURED
Self-powered sensor
ePAD
description In this work, we present a novel self-powered approach totally independent from any external energy source. We have developed a self-powered paper-based immunosensor that generates energy in the presence of the biomarker in the sample. In particular, the device - which has been labeled as Immuno-Battery - makes use of magnesium as anode and the widely employed HRP-labeled antibody as cathodic catalyst to detect C-reactive protein (CRP) presence in artificial samples. Feasibility of self-powered sensing is proved by submitting the immuno-battery to a resistive load. In this regime, the sensor provides operation voltages above 1.55 V and maximum power densities from 40 to 571 μW cm-2 that allow for future implementation of an electronic readout circuit. We have demonstrated that sensitivity of the system is not compromised by the self-powered mode operation, as the LOD value delivered by our battery (20 ± 2 ng mL-1) is compliant with LOD values reported for protein detection in paper-based electrochemical immunoassays with chronoamperometric methods. Moreover, as a case study, a LOD of 269 ± 39 ng mL-1 is obtained for CRP detection, in accordance with available commercial high-sensitivity CRP detection kits. This proof-of-concept opens the path towards the development of digital diagnostic devices in a sustainable and affordable manner.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/350959
https://api.elsevier.com/content/abstract/scopus_id/85141282193
url http://hdl.handle.net/10261/350959
https://api.elsevier.com/content/abstract/scopus_id/85141282193
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/648518
Biosensors & bioelectronics
https://www.sciencedirect.com/science/article/pii/S0956566322009083
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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