Immuno-battery: A single use self-powered immunosensor for REASSURED diagnostics
In this work, we present a novel self-powered approach totally independent from any external energy source. We have developed a self-powered paper-based immunosensor that generates energy in the presence of the biomarker in the sample. In particular, the device - which has been labeled as Immuno-Bat...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/350959 |
| Acceso en línea: | http://hdl.handle.net/10261/350959 https://api.elsevier.com/content/abstract/scopus_id/85141282193 |
| Access Level: | acceso abierto |
| Palabra clave: | Diagnostics Immunoassay REASSURED Self-powered sensor ePAD |
| Sumario: | In this work, we present a novel self-powered approach totally independent from any external energy source. We have developed a self-powered paper-based immunosensor that generates energy in the presence of the biomarker in the sample. In particular, the device - which has been labeled as Immuno-Battery - makes use of magnesium as anode and the widely employed HRP-labeled antibody as cathodic catalyst to detect C-reactive protein (CRP) presence in artificial samples. Feasibility of self-powered sensing is proved by submitting the immuno-battery to a resistive load. In this regime, the sensor provides operation voltages above 1.55 V and maximum power densities from 40 to 571 μW cm-2 that allow for future implementation of an electronic readout circuit. We have demonstrated that sensitivity of the system is not compromised by the self-powered mode operation, as the LOD value delivered by our battery (20 ± 2 ng mL-1) is compliant with LOD values reported for protein detection in paper-based electrochemical immunoassays with chronoamperometric methods. Moreover, as a case study, a LOD of 269 ± 39 ng mL-1 is obtained for CRP detection, in accordance with available commercial high-sensitivity CRP detection kits. This proof-of-concept opens the path towards the development of digital diagnostic devices in a sustainable and affordable manner. |
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