Enhancing alginate dialdehyde-gelatin (ADA-GEL) based hydrogels for biofabrication by addition of phytotherapeutics and mesoporous bioactive glass nanoparticles (MBGNs)

This study explores the 3D printing of alginate dialdehyde-gelatin (ADA-GEL) inks incorporating phytotherapeutic agents, such as ferulic acid (FA), and silicate mesoporous bioactive glass nanoparticles (MBGNs) at two different concentrations. 3D scaffolds with bioactive properties suitable for bone...

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Detalles Bibliográficos
Autores: Bider, Faina, Gunnella, Chiara, Reh, Jana T., Clejanu, Corina-Elena, Kuth, Sonja, Beltrán, Ana M., Boccaccini, Aldo R.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/164233
Acceso en línea:https://hdl.handle.net/11441/164233
https://doi.org/10.1177/08853282241280768
Access Level:acceso abierto
Palabra clave:Hydrogels
Bioactive glass particles
Phytotherapeutic agent
3D (bio)printing
Drug delivery
Bone tissue engineering
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spelling Enhancing alginate dialdehyde-gelatin (ADA-GEL) based hydrogels for biofabrication by addition of phytotherapeutics and mesoporous bioactive glass nanoparticles (MBGNs)Bider, FainaGunnella, ChiaraReh, Jana T.Clejanu, Corina-ElenaKuth, SonjaBeltrán, Ana M.Boccaccini, Aldo R.HydrogelsBioactive glass particlesPhytotherapeutic agent3D (bio)printingDrug deliveryBone tissue engineeringThis study explores the 3D printing of alginate dialdehyde-gelatin (ADA-GEL) inks incorporating phytotherapeutic agents, such as ferulic acid (FA), and silicate mesoporous bioactive glass nanoparticles (MBGNs) at two different concentrations. 3D scaffolds with bioactive properties suitable for bone tissue engineering (TE) were obtained. The degradation and swelling behaviour of films and 3D printed scaffolds indicated an accelerated trend with increasing MBGN content, while FA appeared to stabilize the samples. Determination of the degree of crosslinking validated the increased stability of hydrogels due to the addition of FA and 0.1% (w/v) MBGNs. The incorporation of MBGNs not only improved the effective moduli and conferred bioactive properties through the formation of hydroxyapatite (HAp) on the surface of ADA-GEL-based samples but also enhanced VEGF-A expression of MC3T3-E1 cells. The beneficial impact of FA and low concentrations of MBGNs in ADA-GEL-based inks for 3D (bio)printing applications was corroborated through various printing experiments, resulting in higher printing resolution, as also confirmed by rheological measurements. Cytocompatibility investigations revealed enhanced MC3T3-E1 cell activity and viability. Furthermore, the presence of mineral phases, as confirmed by an in vitro biomineralization assay, and increased ALP activity after 21 days, attributed to the addition of FA and MBGNs, were demonstrated. Considering the acquired structural and biological properties, along with efficient drug delivery capability, enhanced biological activity, and improved 3D printability, the newly developed inks exhibit promising potential for biofabrication and bone TE.University of Erlangen- NurembergCITIUS, central services of the Universidad de Sevilla (Spain)SAGE PublicationsIngeniería y Ciencia de los Materiales y del TransporteTEP123: Metalurgia e Ingeniería de los Materiales2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/164233https://doi.org/10.1177/08853282241280768reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Biomaterials Applications, 9 (6), 524-556.https://journals.sagepub.com/doi/full/10.1177/08853282241280768info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1642332026-06-17T12:51:07Z
dc.title.none.fl_str_mv Enhancing alginate dialdehyde-gelatin (ADA-GEL) based hydrogels for biofabrication by addition of phytotherapeutics and mesoporous bioactive glass nanoparticles (MBGNs)
title Enhancing alginate dialdehyde-gelatin (ADA-GEL) based hydrogels for biofabrication by addition of phytotherapeutics and mesoporous bioactive glass nanoparticles (MBGNs)
spellingShingle Enhancing alginate dialdehyde-gelatin (ADA-GEL) based hydrogels for biofabrication by addition of phytotherapeutics and mesoporous bioactive glass nanoparticles (MBGNs)
Bider, Faina
Hydrogels
Bioactive glass particles
Phytotherapeutic agent
3D (bio)printing
Drug delivery
Bone tissue engineering
title_short Enhancing alginate dialdehyde-gelatin (ADA-GEL) based hydrogels for biofabrication by addition of phytotherapeutics and mesoporous bioactive glass nanoparticles (MBGNs)
title_full Enhancing alginate dialdehyde-gelatin (ADA-GEL) based hydrogels for biofabrication by addition of phytotherapeutics and mesoporous bioactive glass nanoparticles (MBGNs)
title_fullStr Enhancing alginate dialdehyde-gelatin (ADA-GEL) based hydrogels for biofabrication by addition of phytotherapeutics and mesoporous bioactive glass nanoparticles (MBGNs)
title_full_unstemmed Enhancing alginate dialdehyde-gelatin (ADA-GEL) based hydrogels for biofabrication by addition of phytotherapeutics and mesoporous bioactive glass nanoparticles (MBGNs)
title_sort Enhancing alginate dialdehyde-gelatin (ADA-GEL) based hydrogels for biofabrication by addition of phytotherapeutics and mesoporous bioactive glass nanoparticles (MBGNs)
dc.creator.none.fl_str_mv Bider, Faina
Gunnella, Chiara
Reh, Jana T.
Clejanu, Corina-Elena
Kuth, Sonja
Beltrán, Ana M.
Boccaccini, Aldo R.
author Bider, Faina
author_facet Bider, Faina
Gunnella, Chiara
Reh, Jana T.
Clejanu, Corina-Elena
Kuth, Sonja
Beltrán, Ana M.
Boccaccini, Aldo R.
author_role author
author2 Gunnella, Chiara
Reh, Jana T.
Clejanu, Corina-Elena
Kuth, Sonja
Beltrán, Ana M.
Boccaccini, Aldo R.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ingeniería y Ciencia de los Materiales y del Transporte
TEP123: Metalurgia e Ingeniería de los Materiales
dc.subject.none.fl_str_mv Hydrogels
Bioactive glass particles
Phytotherapeutic agent
3D (bio)printing
Drug delivery
Bone tissue engineering
topic Hydrogels
Bioactive glass particles
Phytotherapeutic agent
3D (bio)printing
Drug delivery
Bone tissue engineering
description This study explores the 3D printing of alginate dialdehyde-gelatin (ADA-GEL) inks incorporating phytotherapeutic agents, such as ferulic acid (FA), and silicate mesoporous bioactive glass nanoparticles (MBGNs) at two different concentrations. 3D scaffolds with bioactive properties suitable for bone tissue engineering (TE) were obtained. The degradation and swelling behaviour of films and 3D printed scaffolds indicated an accelerated trend with increasing MBGN content, while FA appeared to stabilize the samples. Determination of the degree of crosslinking validated the increased stability of hydrogels due to the addition of FA and 0.1% (w/v) MBGNs. The incorporation of MBGNs not only improved the effective moduli and conferred bioactive properties through the formation of hydroxyapatite (HAp) on the surface of ADA-GEL-based samples but also enhanced VEGF-A expression of MC3T3-E1 cells. The beneficial impact of FA and low concentrations of MBGNs in ADA-GEL-based inks for 3D (bio)printing applications was corroborated through various printing experiments, resulting in higher printing resolution, as also confirmed by rheological measurements. Cytocompatibility investigations revealed enhanced MC3T3-E1 cell activity and viability. Furthermore, the presence of mineral phases, as confirmed by an in vitro biomineralization assay, and increased ALP activity after 21 days, attributed to the addition of FA and MBGNs, were demonstrated. Considering the acquired structural and biological properties, along with efficient drug delivery capability, enhanced biological activity, and improved 3D printability, the newly developed inks exhibit promising potential for biofabrication and bone TE.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/164233
https://doi.org/10.1177/08853282241280768
url https://hdl.handle.net/11441/164233
https://doi.org/10.1177/08853282241280768
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Biomaterials Applications, 9 (6), 524-556.
https://journals.sagepub.com/doi/full/10.1177/08853282241280768
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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