Bioelectrocatalytic CO2 reduction to formate by Candida boidinii formate dehydrogenase overcoming NADH dependence with tailored amino-viologen redox polymers

CO2 reduction to formate through enzymatic systems represents a sustainable pathway for carbon utilization but is often limited by the cost and irreversibility of cofactors like NADH. In this study, we introduce a novel biocathode integrating NAD-dependent formate dehydrogenase (cbFDH) and an amino-...

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Detalles Bibliográficos
Autores: Fera, Mihai Cristian, Jayakumar, Kavita, García Bueno, Diego, Abad, Jose M., De Lacey, Antonio L., Pita, Marcos
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/399828
Acceso en línea:http://hdl.handle.net/10261/399828
https://api.elsevier.com/content/abstract/scopus_id/85217657121
Access Level:acceso abierto
Palabra clave:Bioelectrochemistry
CO2 reduction
Formate dehydrogenase
NADH substitution
Redox polymer
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spelling Bioelectrocatalytic CO2 reduction to formate by Candida boidinii formate dehydrogenase overcoming NADH dependence with tailored amino-viologen redox polymersFera, Mihai CristianJayakumar, KavitaGarcía Bueno, DiegoAbad, Jose M.De Lacey, Antonio L.Pita, MarcosBioelectrochemistryCO2 reductionFormate dehydrogenaseNADH substitutionRedox polymerCO2 reduction to formate through enzymatic systems represents a sustainable pathway for carbon utilization but is often limited by the cost and irreversibility of cofactors like NADH. In this study, we introduce a novel biocathode integrating NAD-dependent formate dehydrogenase (cbFDH) and an amino-viologen redox polymer (NH2Et-PVI) to act as a mediating artificial cofactor, enabling continuous formate production without re-supply of exogenous cofactors. This bioelectrode achieves a faradaic efficiency of 95.4 % and a 43-fold increase in formate yield over traditional NADH-dependent biocatalytic systems, which highlights the cbFDH/NH2Et-PVI bioelectrode as a promising advancement for economically viable CO2 conversion.M-C. Fera and M. Pita acknowledge TED2021-129694B-C22 funded by MCIN/AEI/10.13039/501100011033 by the European Union Next GenerationEU/PRTR. K. Jayakumar acknowledges BPYMAC project funded by the European Union (HORIZON-MSCA-2023-PF-01-01/101154827). J.M. Abad and A.L de Lacey. acknowledge the CEOTRES-CM project funded by the Comunidad de Madrid with Grant Number Y2020/EMT-6419.Peer reviewedElsevier BVAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)European CommissionComunidad de MadridFera, Mihai Cristian [0000-0003-3011-2575]De Lacey, Antonio L. [0000-0002-9347-0452]Pita, Marcos [0000-0002-6714-3669]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/399828https://api.elsevier.com/content/abstract/scopus_id/85217657121reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//TED2021-129694B-C22info:eu-repo/grantAgreement/EC/HE/101154827Y2020/EMT-6419/CEOTRES-CMThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.jcou.2025.103041https://doi.org/10.1016/j.jcou.2025.103041Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3998282026-05-22T06:33:51Z
dc.title.none.fl_str_mv Bioelectrocatalytic CO2 reduction to formate by Candida boidinii formate dehydrogenase overcoming NADH dependence with tailored amino-viologen redox polymers
title Bioelectrocatalytic CO2 reduction to formate by Candida boidinii formate dehydrogenase overcoming NADH dependence with tailored amino-viologen redox polymers
spellingShingle Bioelectrocatalytic CO2 reduction to formate by Candida boidinii formate dehydrogenase overcoming NADH dependence with tailored amino-viologen redox polymers
Fera, Mihai Cristian
Bioelectrochemistry
CO2 reduction
Formate dehydrogenase
NADH substitution
Redox polymer
title_short Bioelectrocatalytic CO2 reduction to formate by Candida boidinii formate dehydrogenase overcoming NADH dependence with tailored amino-viologen redox polymers
title_full Bioelectrocatalytic CO2 reduction to formate by Candida boidinii formate dehydrogenase overcoming NADH dependence with tailored amino-viologen redox polymers
title_fullStr Bioelectrocatalytic CO2 reduction to formate by Candida boidinii formate dehydrogenase overcoming NADH dependence with tailored amino-viologen redox polymers
title_full_unstemmed Bioelectrocatalytic CO2 reduction to formate by Candida boidinii formate dehydrogenase overcoming NADH dependence with tailored amino-viologen redox polymers
title_sort Bioelectrocatalytic CO2 reduction to formate by Candida boidinii formate dehydrogenase overcoming NADH dependence with tailored amino-viologen redox polymers
dc.creator.none.fl_str_mv Fera, Mihai Cristian
Jayakumar, Kavita
García Bueno, Diego
Abad, Jose M.
De Lacey, Antonio L.
Pita, Marcos
author Fera, Mihai Cristian
author_facet Fera, Mihai Cristian
Jayakumar, Kavita
García Bueno, Diego
Abad, Jose M.
De Lacey, Antonio L.
Pita, Marcos
author_role author
author2 Jayakumar, Kavita
García Bueno, Diego
Abad, Jose M.
De Lacey, Antonio L.
Pita, Marcos
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Comunidad de Madrid
Fera, Mihai Cristian [0000-0003-3011-2575]
De Lacey, Antonio L. [0000-0002-9347-0452]
Pita, Marcos [0000-0002-6714-3669]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bioelectrochemistry
CO2 reduction
Formate dehydrogenase
NADH substitution
Redox polymer
topic Bioelectrochemistry
CO2 reduction
Formate dehydrogenase
NADH substitution
Redox polymer
description CO2 reduction to formate through enzymatic systems represents a sustainable pathway for carbon utilization but is often limited by the cost and irreversibility of cofactors like NADH. In this study, we introduce a novel biocathode integrating NAD-dependent formate dehydrogenase (cbFDH) and an amino-viologen redox polymer (NH2Et-PVI) to act as a mediating artificial cofactor, enabling continuous formate production without re-supply of exogenous cofactors. This bioelectrode achieves a faradaic efficiency of 95.4 % and a 43-fold increase in formate yield over traditional NADH-dependent biocatalytic systems, which highlights the cbFDH/NH2Et-PVI bioelectrode as a promising advancement for economically viable CO2 conversion.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/399828
https://api.elsevier.com/content/abstract/scopus_id/85217657121
url http://hdl.handle.net/10261/399828
https://api.elsevier.com/content/abstract/scopus_id/85217657121
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//TED2021-129694B-C22
info:eu-repo/grantAgreement/EC/HE/101154827
Y2020/EMT-6419/CEOTRES-CM
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.jcou.2025.103041
https://doi.org/10.1016/j.jcou.2025.103041
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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