Bioelectrocatalytic CO2 reduction to formate by Candida boidinii formate dehydrogenase overcoming NADH dependence with tailored amino-viologen redox polymers
CO2 reduction to formate through enzymatic systems represents a sustainable pathway for carbon utilization but is often limited by the cost and irreversibility of cofactors like NADH. In this study, we introduce a novel biocathode integrating NAD-dependent formate dehydrogenase (cbFDH) and an amino-...
| Authors: | , , , , , |
|---|---|
| Format: | article |
| Status: | Published version |
| Publication Date: | 2025 |
| Country: | España |
| Institution: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repository: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/399828 |
| Online Access: | http://hdl.handle.net/10261/399828 https://api.elsevier.com/content/abstract/scopus_id/85217657121 |
| Access Level: | Open access |
| Keyword: | Bioelectrochemistry CO2 reduction Formate dehydrogenase NADH substitution Redox polymer |
| Summary: | CO2 reduction to formate through enzymatic systems represents a sustainable pathway for carbon utilization but is often limited by the cost and irreversibility of cofactors like NADH. In this study, we introduce a novel biocathode integrating NAD-dependent formate dehydrogenase (cbFDH) and an amino-viologen redox polymer (NH2Et-PVI) to act as a mediating artificial cofactor, enabling continuous formate production without re-supply of exogenous cofactors. This bioelectrode achieves a faradaic efficiency of 95.4 % and a 43-fold increase in formate yield over traditional NADH-dependent biocatalytic systems, which highlights the cbFDH/NH2Et-PVI bioelectrode as a promising advancement for economically viable CO2 conversion. |
|---|