A new method to simultaneously quantify the antioxidants: carotenes, xanthophylls, and vitamin A in human plasma

A simple and accurate reversed phase high-performance liquid chromatography coupled with diode array detector (HPLC-DAD) method for simultaneously determining and quantifying the antioxidants carotenes, xanthophylls, and retinol in human plasma is presented in this paper. Compounds were extracted wi...

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Detalhes bibliográficos
Autores: Colmán Martínez, Lourdes Mariel, Martínez Huélamo, Miriam, Miralles, Esther, Estruch Riba, Ramon, Lamuela Raventós, Rosa Ma.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/119182
Acesso em linha:https://hdl.handle.net/2445/119182
Access Level:acceso abierto
Palavra-chave:Cromatografia de líquids
Antioxidants
Carotenoides
Vitamina A
Plasma sanguini
Liquid chromatography
Carotenoids
Vitamin A
Blood plasma
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spelling A new method to simultaneously quantify the antioxidants: carotenes, xanthophylls, and vitamin A in human plasmaColmán Martínez, Lourdes MarielMartínez Huélamo, MiriamMiralles, EstherEstruch Riba, RamonLamuela Raventós, Rosa Ma.Cromatografia de líquidsAntioxidantsCarotenoidesVitamina APlasma sanguiniLiquid chromatographyAntioxidantsCarotenoidsVitamin ABlood plasmaA simple and accurate reversed phase high-performance liquid chromatography coupled with diode array detector (HPLC-DAD) method for simultaneously determining and quantifying the antioxidants carotenes, xanthophylls, and retinol in human plasma is presented in this paper. Compounds were extracted with hexane, a C30 column, and a mobile phase of methanol, methyl tert-butyl ether, and water were used for the separation of the compounds. A total of 8 carotenoids, 3 Z--carotene isomers, and 1 fat-soluble vitamin (retinol) were resolved within 72 min at a flow rate of 0.6 mL/min. Detection was achieved at 450 nm for carotenoids and 330 nm for retinol. To evaluate the effectiveness of themethod, it has been applied to an intervention study conducted on eight volunteers. Results. Limits of detection were between 0.1 g/mL for lycopene and astaxanthin and 1.3 g/mL for 15-Z--carotene. Recoveries were ranged between 89% and 113% for -carotene and astaxanthin, respectively. Accuracy was between 90.7% and 112.2% and precision was between 1% and 15% RSD. In human plasma samples compounds studied were identified besides three lycopene isomers, demonstrated to be suitable for application in dietary intervention studies. Conclusions. Due to its accuracy, precision, selectivity, and reproducibility, this method is suitable to dietary habits and/or antioxidants status studies.Hindawi2018201820152018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 p.application/pdfhttps://hdl.handle.net/2445/119182Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1155/2016/9268531Oxidative Medicine and Cellular Longevity, 2015, vol. 2015, num. 9268531https://doi.org/10.1155/2016/9268531cc-by (c) Colmán Martínez, Lourdes Mariel et al., 2015http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1191822026-05-29T05:05:01Z
dc.title.none.fl_str_mv A new method to simultaneously quantify the antioxidants: carotenes, xanthophylls, and vitamin A in human plasma
title A new method to simultaneously quantify the antioxidants: carotenes, xanthophylls, and vitamin A in human plasma
spellingShingle A new method to simultaneously quantify the antioxidants: carotenes, xanthophylls, and vitamin A in human plasma
Colmán Martínez, Lourdes Mariel
Cromatografia de líquids
Antioxidants
Carotenoides
Vitamina A
Plasma sanguini
Liquid chromatography
Antioxidants
Carotenoids
Vitamin A
Blood plasma
title_short A new method to simultaneously quantify the antioxidants: carotenes, xanthophylls, and vitamin A in human plasma
title_full A new method to simultaneously quantify the antioxidants: carotenes, xanthophylls, and vitamin A in human plasma
title_fullStr A new method to simultaneously quantify the antioxidants: carotenes, xanthophylls, and vitamin A in human plasma
title_full_unstemmed A new method to simultaneously quantify the antioxidants: carotenes, xanthophylls, and vitamin A in human plasma
title_sort A new method to simultaneously quantify the antioxidants: carotenes, xanthophylls, and vitamin A in human plasma
dc.creator.none.fl_str_mv Colmán Martínez, Lourdes Mariel
Martínez Huélamo, Miriam
Miralles, Esther
Estruch Riba, Ramon
Lamuela Raventós, Rosa Ma.
author Colmán Martínez, Lourdes Mariel
author_facet Colmán Martínez, Lourdes Mariel
Martínez Huélamo, Miriam
Miralles, Esther
Estruch Riba, Ramon
Lamuela Raventós, Rosa Ma.
author_role author
author2 Martínez Huélamo, Miriam
Miralles, Esther
Estruch Riba, Ramon
Lamuela Raventós, Rosa Ma.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cromatografia de líquids
Antioxidants
Carotenoides
Vitamina A
Plasma sanguini
Liquid chromatography
Antioxidants
Carotenoids
Vitamin A
Blood plasma
topic Cromatografia de líquids
Antioxidants
Carotenoides
Vitamina A
Plasma sanguini
Liquid chromatography
Antioxidants
Carotenoids
Vitamin A
Blood plasma
description A simple and accurate reversed phase high-performance liquid chromatography coupled with diode array detector (HPLC-DAD) method for simultaneously determining and quantifying the antioxidants carotenes, xanthophylls, and retinol in human plasma is presented in this paper. Compounds were extracted with hexane, a C30 column, and a mobile phase of methanol, methyl tert-butyl ether, and water were used for the separation of the compounds. A total of 8 carotenoids, 3 Z--carotene isomers, and 1 fat-soluble vitamin (retinol) were resolved within 72 min at a flow rate of 0.6 mL/min. Detection was achieved at 450 nm for carotenoids and 330 nm for retinol. To evaluate the effectiveness of themethod, it has been applied to an intervention study conducted on eight volunteers. Results. Limits of detection were between 0.1 g/mL for lycopene and astaxanthin and 1.3 g/mL for 15-Z--carotene. Recoveries were ranged between 89% and 113% for -carotene and astaxanthin, respectively. Accuracy was between 90.7% and 112.2% and precision was between 1% and 15% RSD. In human plasma samples compounds studied were identified besides three lycopene isomers, demonstrated to be suitable for application in dietary intervention studies. Conclusions. Due to its accuracy, precision, selectivity, and reproducibility, this method is suitable to dietary habits and/or antioxidants status studies.
publishDate 2015
dc.date.none.fl_str_mv 2015
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/119182
url https://hdl.handle.net/2445/119182
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1155/2016/9268531
Oxidative Medicine and Cellular Longevity, 2015, vol. 2015, num. 9268531
https://doi.org/10.1155/2016/9268531
dc.rights.none.fl_str_mv cc-by (c) Colmán Martínez, Lourdes Mariel et al., 2015
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Colmán Martínez, Lourdes Mariel et al., 2015
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Hindawi
publisher.none.fl_str_mv Hindawi
dc.source.none.fl_str_mv Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407663135129600
score 15,811543

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