Advanced strategy to exploit wine-making waste by manufacturing antioxidant and prebiotic fibre-enriched vesicles for intestinal health
Grape extract-loaded fibre-enriched vesicles, nutriosomes, were prepared by combining antioxidant extracts obtained from grape pomaces and a prebiotic, soluble fibre (Nutriose (R) FM06). The nutriosomes were small in size (from similar to 140 to 260 nm), homogeneous (polydispersity index < 0.2) a...
| Autores: | , , , , , , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2020 |
| País: | España |
| Recursos: | Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO) |
| Repositório: | r-FISABIO. Repositorio Institucional de Producción Científica |
| OAI Identifier: | oai:fisabio.fundanetsuite.com:p7435 |
| Acesso em linha: | https://fisabio.portalinvestigacion.com/publicaciones/7435 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Grape pomace Phospholipid vesicles Nutriosomes Antioxidant activity Prebiotic activity Gut microbiota Intestinal cells In vivo studies |
| Resumo: | Grape extract-loaded fibre-enriched vesicles, nutriosomes, were prepared by combining antioxidant extracts obtained from grape pomaces and a prebiotic, soluble fibre (Nutriose (R) FM06). The nutriosomes were small in size (from similar to 140 to 260 nm), homogeneous (polydispersity index < 0.2) and highly negative (similar to -79 mV). The vesicles were highly stable during 12 months of storage at 25 degrees C. When diluted with warmed (37 degrees C) acidic medium (pH 1.2) of high ionic strength, the vesicles only displayed an increase of the mean diameter and a low release of the extract, which were dependent on Nutriose concentration. The formulations were highly bio-compatible and able to protect intestinal cells (Caco-2) from oxidative stress damage. In vivo results underlined that the composition of mouse microbiota was not affected by the vesicular formulations. Overall results support the potential application of grape nutriosomes as an alternative strategy for the protection of the intestinal tract. |
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