Profiling of oxygen in biofilms using individually addressable disk microelectrodes on a microfabricated needle

A novel microelectrode array sensor was fabricated using MEMS technology on a needle, and then applied to real-time measurement of dissolved oxygen (DO) inside biofilms. The sensor consists of eleven gold disk microelectrodes, a rectangular auxiliary electrode along them, and an external and interna...

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Detalles Bibliográficos
Autores: Moya, Ana, Guimerà, Xavier, del Campo, Francisco Javier, Prats-Alfonso, Elisabet, Dorado, Antonio David, Baeza, Mireia, Villa, Rosa, Gabriel, David, Gamisans, Xavier, Gabriel, Gemma
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/414903
Acceso en línea:http://hdl.handle.net/10261/414903
https://api.elsevier.com/content/abstract/scopus_id/84925483661
Access Level:acceso abierto
Palabra clave:Biofilms
Bioprocess monitoring
Dissolved oxygen
Microelectrode array
http://metadata.un.org/sdg/9
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
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spelling Profiling of oxygen in biofilms using individually addressable disk microelectrodes on a microfabricated needleMoya, AnaGuimerà, Xavierdel Campo, Francisco JavierPrats-Alfonso, ElisabetDorado, Antonio DavidBaeza, MireiaVilla, RosaGabriel, DavidGamisans, XavierGabriel, GemmaBiofilmsBioprocess monitoringDissolved oxygenMicroelectrode arrayhttp://metadata.un.org/sdg/9Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovationA novel microelectrode array sensor was fabricated using MEMS technology on a needle, and then applied to real-time measurement of dissolved oxygen (DO) inside biofilms. The sensor consists of eleven gold disk microelectrodes, a rectangular auxiliary electrode along them, and an external and internal reference electrode. Three kinds of sensors were designed and their responses were characterized and evaluated under various conditions. The arrays exhibit a linear response to DO in the 0–8 mg·L<sup>−1</sup> concentration range in water, high sensitivity, repeatability, and low limits of detection (<0.11 mg·L<sup>−1</sup> of DO) and quantification (0.38 mg·L<sup>−1</sup> DO). The sensors were validated against a commercial Clark-type microelectrode and applied to profiling of DO in a heterotrophic biofilm cultivated in a flat-plate bioreactor. It is shown that the sensor array can provide a multipoint, simultaneous snapshot profile of DO inside a biofilm with high spatial resolution due to its micrometric dimensions. We conclude that this new sensor array represents a powerful tool for sensing of DO biofilms and in numerous bioprocesses.[Figure not available: see fulltext.]This work has been founded by projects DPI2011-28262-C04 and CTM2012-37927-C03/FEDER, financed by the Ministerio de Economía y Competitividad (Spain). AM gratefully ac-knowledges an FPI-2012 pre-doctoral scholarship, (it funded her PhD studies at Universitat Autònoma de Barcelona), and XG also acknowl- edges an FPI-UPC pre-doctoral scholarship, both from Ministerio de Economía y Competitividad (Spain).Peer reviewedSpringer NatureMinisterio de Economía y Competitividad (España)#NODATA##NODATA##NODATA##NODATA##NODATA##NODATA##NODATA##NODATA##NODATA##NODATA#Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/414903https://api.elsevier.com/content/abstract/scopus_id/84925483661reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN//DPI2011-28262-C04-02info:eu-repo/grantAgreement/MICINN//DPI2011-28262-C04-01info:eu-repo/grantAgreement/MINECO//CTM2012-37927-C03-03info:eu-repo/grantAgreement/MINECO//CTM2012-37927-C03-01Microchimica Actahttps://link.springer.com/article/10.1007/s00604-014-1405-4Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4149032026-05-22T06:33:51Z
dc.title.none.fl_str_mv Profiling of oxygen in biofilms using individually addressable disk microelectrodes on a microfabricated needle
title Profiling of oxygen in biofilms using individually addressable disk microelectrodes on a microfabricated needle
spellingShingle Profiling of oxygen in biofilms using individually addressable disk microelectrodes on a microfabricated needle
Moya, Ana
Biofilms
Bioprocess monitoring
Dissolved oxygen
Microelectrode array
http://metadata.un.org/sdg/9
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
title_short Profiling of oxygen in biofilms using individually addressable disk microelectrodes on a microfabricated needle
title_full Profiling of oxygen in biofilms using individually addressable disk microelectrodes on a microfabricated needle
title_fullStr Profiling of oxygen in biofilms using individually addressable disk microelectrodes on a microfabricated needle
title_full_unstemmed Profiling of oxygen in biofilms using individually addressable disk microelectrodes on a microfabricated needle
title_sort Profiling of oxygen in biofilms using individually addressable disk microelectrodes on a microfabricated needle
dc.creator.none.fl_str_mv Moya, Ana
Guimerà, Xavier
del Campo, Francisco Javier
Prats-Alfonso, Elisabet
Dorado, Antonio David
Baeza, Mireia
Villa, Rosa
Gabriel, David
Gamisans, Xavier
Gabriel, Gemma
author Moya, Ana
author_facet Moya, Ana
Guimerà, Xavier
del Campo, Francisco Javier
Prats-Alfonso, Elisabet
Dorado, Antonio David
Baeza, Mireia
Villa, Rosa
Gabriel, David
Gamisans, Xavier
Gabriel, Gemma
author_role author
author2 Guimerà, Xavier
del Campo, Francisco Javier
Prats-Alfonso, Elisabet
Dorado, Antonio David
Baeza, Mireia
Villa, Rosa
Gabriel, David
Gamisans, Xavier
Gabriel, Gemma
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biofilms
Bioprocess monitoring
Dissolved oxygen
Microelectrode array
http://metadata.un.org/sdg/9
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
topic Biofilms
Bioprocess monitoring
Dissolved oxygen
Microelectrode array
http://metadata.un.org/sdg/9
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
description A novel microelectrode array sensor was fabricated using MEMS technology on a needle, and then applied to real-time measurement of dissolved oxygen (DO) inside biofilms. The sensor consists of eleven gold disk microelectrodes, a rectangular auxiliary electrode along them, and an external and internal reference electrode. Three kinds of sensors were designed and their responses were characterized and evaluated under various conditions. The arrays exhibit a linear response to DO in the 0–8 mg·L<sup>−1</sup> concentration range in water, high sensitivity, repeatability, and low limits of detection (<0.11 mg·L<sup>−1</sup> of DO) and quantification (0.38 mg·L<sup>−1</sup> DO). The sensors were validated against a commercial Clark-type microelectrode and applied to profiling of DO in a heterotrophic biofilm cultivated in a flat-plate bioreactor. It is shown that the sensor array can provide a multipoint, simultaneous snapshot profile of DO inside a biofilm with high spatial resolution due to its micrometric dimensions. We conclude that this new sensor array represents a powerful tool for sensing of DO biofilms and in numerous bioprocesses.[Figure not available: see fulltext.]
publishDate 2015
dc.date.none.fl_str_mv 2015
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/414903
https://api.elsevier.com/content/abstract/scopus_id/84925483661
url http://hdl.handle.net/10261/414903
https://api.elsevier.com/content/abstract/scopus_id/84925483661
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN//DPI2011-28262-C04-02
info:eu-repo/grantAgreement/MICINN//DPI2011-28262-C04-01
info:eu-repo/grantAgreement/MINECO//CTM2012-37927-C03-03
info:eu-repo/grantAgreement/MINECO//CTM2012-37927-C03-01
Microchimica Acta
https://link.springer.com/article/10.1007/s00604-014-1405-4
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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