Profiling of oxygen in biofilms using individually addressable disk microelectrodes on a microfabricated needle
A novel microelectrode array sensor was fabricated using MEMS technology on a needle, and then applied to real-time measurement of dissolved oxygen (DO) inside biofilms. The sensor consists of eleven gold disk microelectrodes and a rectangular auxiliary electrode along them and an external & int...
| Autores: | , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/27553 |
| Acceso en línea: | https://hdl.handle.net/2117/27553 https://dx.doi.org/10.1007/s00604-014-1405-4 |
| Access Level: | acceso abierto |
| Palabra clave: | Dissolved Oxygen Microelectrode Array Sensors Bioprocess Monitoring Biofilms Àrees temàtiques de la UPC::Enginyeria química::Química del medi ambient Àrees temàtiques de la UPC::Enginyeria química::Química analítica |
| Sumario: | A novel microelectrode array sensor was fabricated using MEMS technology on a needle, and then applied to real-time measurement of dissolved oxygen (DO) inside biofilms. The sensor consists of eleven gold disk microelectrodes and a rectangular auxiliary electrode along them and an external & internal reference electrode. Three kinds of sensors were designed and their responses were characterized and evaluated under various environmental conditions. The arrays exhibit a linear response to DO in the 0 - 8 mg L-1 concentration range in water, high sensitivity, repeatability, and low limits of detection (< 0.11 mg L-1) and quantification (0.38 mg L-1). The sensors were then validated against a commercial Clark-type microelectrode and applied to profiling of DO in a heterotrophic biofilm cultivated in a flat-plate bioreactor. It is shown that the sensor array can provide a multipoint, simultaneous snapshot profile of DO inside a biofilm with high spatial resolution due to its micrometric dimensions. We conclude that this new sensor array represents a powerful tool for sensing of DO biofilms and in numerous bioprocesses. |
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