Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine

Aims: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leadi...

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Autores: Barregard, L, Moller, P, Henriksen, T, Mistry, V, Koppen, G, Rossner, P, Sram, RJ, Weimann, A, Poulsen, HE, Nataf, R, Andreoli, R, Manini, P, Marczylo, T, Lam, P, Evans, MD, Kasai, H, Kawai, K, Li, YS, Sakai, K, Singh, R, Teichert, F, Farmer, PB, Rozalski, R, Gackowski, D, Siomek, A, Saez, GT, Cerda, C, Broberg, K, Lindh, C, Hossain, MB, Haghdoost, S, Hu, CW, Chao, MR, Wu, KY, Orhan, H, Senduran, N, Smith, RJ, Santella, RM, Su, YL, Cortez, C, Yeh, S, Olinski, R, Loft, S, Cooke, MS
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p10679
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/10679
Access Level:acceso abierto
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spelling Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosineBarregard, LMoller, PHenriksen, TMistry, VKoppen, GRossner, PSram, RJWeimann, APoulsen, HENataf, RAndreoli, RManini, PMarczylo, TLam, PEvans, MDKasai, HKawai, KLi, YSSakai, KSingh, RTeichert, FFarmer, PBRozalski, RGackowski, DSiomek, ASaez, GTCerda, CBroberg, KLindh, CHossain, MBHaghdoost, SHu, CWChao, MRWu, KYOrhan, HSenduran, NSmith, RJSantella, RMSu, YLCortez, CYeh, SOlinski, RLoft, SCooke, MSAims: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability. Results: Chromatographic assays showed high agreement across urines from different subjects, whereas ELISAs showed far more inter-laboratory variation and generally overestimated levels, compared to the chromatographic assays. Excretion rates in timed 'spot' samples showed strong correlations with 24 h excretion (the 'gold' standard) of urinary 8-oxodG (r(p) 0.67-0.90), although the associations were weaker for 8-oxodG adjusted for creatinine or specific gravity (SG). The within-individual excretion of 8-oxodG varied only moderately between days (CV 17% for 24 h excretion and 20% for first void, creatinine-corrected samples). Innovation: This is the first comprehensive study of both human and methodological factors influencing 8-oxodG measurement, providing key information for future studies with this important biomarker. Conclusion: ELISA variability is greater than chromatographic assay variability, and cannot determine absolute levels of 8-oxodG. Use of standardized calibrants greatly improves intra-technique agreement and, for the chromatographic assays, importantly allows integration of results for pooled analyses. If 24 h samples are not feasible, creatinine- or SG-adjusted first morning samples are recommended.MARY ANN LIEBERT, INC2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/10679ANTIOXIDANTS & REDOX SIGNALINGISSN: 15230864ISSNe: 15577716reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p106792026-06-11T12:45:17Z
dc.title.none.fl_str_mv Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine
title Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine
spellingShingle Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine
Barregard, L
title_short Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine
title_full Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine
title_fullStr Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine
title_full_unstemmed Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine
title_sort Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine
dc.creator.none.fl_str_mv Barregard, L
Moller, P
Henriksen, T
Mistry, V
Koppen, G
Rossner, P
Sram, RJ
Weimann, A
Poulsen, HE
Nataf, R
Andreoli, R
Manini, P
Marczylo, T
Lam, P
Evans, MD
Kasai, H
Kawai, K
Li, YS
Sakai, K
Singh, R
Teichert, F
Farmer, PB
Rozalski, R
Gackowski, D
Siomek, A
Saez, GT
Cerda, C
Broberg, K
Lindh, C
Hossain, MB
Haghdoost, S
Hu, CW
Chao, MR
Wu, KY
Orhan, H
Senduran, N
Smith, RJ
Santella, RM
Su, YL
Cortez, C
Yeh, S
Olinski, R
Loft, S
Cooke, MS
author Barregard, L
author_facet Barregard, L
Moller, P
Henriksen, T
Mistry, V
Koppen, G
Rossner, P
Sram, RJ
Weimann, A
Poulsen, HE
Nataf, R
Andreoli, R
Manini, P
Marczylo, T
Lam, P
Evans, MD
Kasai, H
Kawai, K
Li, YS
Sakai, K
Singh, R
Teichert, F
Farmer, PB
Rozalski, R
Gackowski, D
Siomek, A
Saez, GT
Cerda, C
Broberg, K
Lindh, C
Hossain, MB
Haghdoost, S
Hu, CW
Chao, MR
Wu, KY
Orhan, H
Senduran, N
Smith, RJ
Santella, RM
Su, YL
Cortez, C
Yeh, S
Olinski, R
Loft, S
Cooke, MS
author_role author
author2 Moller, P
Henriksen, T
Mistry, V
Koppen, G
Rossner, P
Sram, RJ
Weimann, A
Poulsen, HE
Nataf, R
Andreoli, R
Manini, P
Marczylo, T
Lam, P
Evans, MD
Kasai, H
Kawai, K
Li, YS
Sakai, K
Singh, R
Teichert, F
Farmer, PB
Rozalski, R
Gackowski, D
Siomek, A
Saez, GT
Cerda, C
Broberg, K
Lindh, C
Hossain, MB
Haghdoost, S
Hu, CW
Chao, MR
Wu, KY
Orhan, H
Senduran, N
Smith, RJ
Santella, RM
Su, YL
Cortez, C
Yeh, S
Olinski, R
Loft, S
Cooke, MS
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
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author
description Aims: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability. Results: Chromatographic assays showed high agreement across urines from different subjects, whereas ELISAs showed far more inter-laboratory variation and generally overestimated levels, compared to the chromatographic assays. Excretion rates in timed 'spot' samples showed strong correlations with 24 h excretion (the 'gold' standard) of urinary 8-oxodG (r(p) 0.67-0.90), although the associations were weaker for 8-oxodG adjusted for creatinine or specific gravity (SG). The within-individual excretion of 8-oxodG varied only moderately between days (CV 17% for 24 h excretion and 20% for first void, creatinine-corrected samples). Innovation: This is the first comprehensive study of both human and methodological factors influencing 8-oxodG measurement, providing key information for future studies with this important biomarker. Conclusion: ELISA variability is greater than chromatographic assay variability, and cannot determine absolute levels of 8-oxodG. Use of standardized calibrants greatly improves intra-technique agreement and, for the chromatographic assays, importantly allows integration of results for pooled analyses. If 24 h samples are not feasible, creatinine- or SG-adjusted first morning samples are recommended.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/10679
url https://fisabio.portalinvestigacion.com/publicaciones/10679
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MARY ANN LIEBERT, INC
publisher.none.fl_str_mv MARY ANN LIEBERT, INC
dc.source.none.fl_str_mv ANTIOXIDANTS & REDOX SIGNALING
ISSN: 15230864
ISSNe: 15577716
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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