Nanoplastic exposure affects the intestinal microbiota of adult Drosophila flies

Rocabert Valverde, Arnau; Martín Pérez, Joan|||0009-0006-9137-3604; Pareras Puig, Laia|||0000-0002-1381-8894; Egea, Raquel|||0000-0002-6201-4112; Alaraby, Mohamed|||0000-0001-7488-3318; Cabrera Gumbau, Jordi Manuel|||0009-0007-3952-7589; Sarmiento Guardia, Iris; Martínez-Urtaza, Jaime|||0000-0001-6219-0418; Rubio Lorente, Laura|||0000-0002-2088-3803; Barguilla, Irene|||0000-0003-1081-4457; Marcos Dauder, Ricardo|||0000-0001-7891-357X; García Rodríguez, Alba|||0000-0002-1175-7418; Hernández Bonilla, Alba|||0000-0001-6938-1233

Nanoplastic exposure affects the intestinal microbiota of adult Drosophila flies

Micro- and nanoplastics (MNPLs) are emerging environmental pollutants that have garnered significant attention over the past few decades due to their detrimental effects on human health through various exposure pathways. This study investigates the impact of MNPLs on gut microbiota, utilizing Drosop...

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Autores:	Rocabert Valverde, Arnau, Martín Pérez, Joan\|\|\|0009-0006-9137-3604, Pareras Puig, Laia\|\|\|0000-0002-1381-8894, Egea, Raquel\|\|\|0000-0002-6201-4112, Alaraby, Mohamed\|\|\|0000-0001-7488-3318, Cabrera Gumbau, Jordi Manuel\|\|\|0009-0007-3952-7589, Sarmiento Guardia, Iris, Martínez-Urtaza, Jaime\|\|\|0000-0001-6219-0418, Rubio Lorente, Laura\|\|\|0000-0002-2088-3803, Barguilla, Irene\|\|\|0000-0003-1081-4457, Marcos Dauder, Ricardo\|\|\|0000-0001-7891-357X, García Rodríguez, Alba\|\|\|0000-0002-1175-7418, Hernández Bonilla, Alba\|\|\|0000-0001-6938-1233
Tipo de recurso:	artículo
Fecha de publicación:	2025
País:	España
Institución:	Universitat Autònoma de Barcelona
Repositorio:	Dipòsit Digital de Documents de la UAB
Idioma:	inglés
OAI Identifier:	oai:ddd.uab.cat:311844
Acceso en línea:	https://ddd.uab.cat/record/311844 https://dx.doi.org/urn:doi:10.1016/j.scitotenv.2025.179545
Access Level:	acceso abierto
Palabra clave:	Drosophila melanogaster MNPLs PCR 16S sequencing MinION Nanopore Microbiota Dysbiosis

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spelling	Nanoplastic exposure affects the intestinal microbiota of adult Drosophila fliesRocabert Valverde, ArnauMartín Pérez, Joan\|\|\|0009-0006-9137-3604Pareras Puig, Laia\|\|\|0000-0002-1381-8894Egea, Raquel\|\|\|0000-0002-6201-4112Alaraby, Mohamed\|\|\|0000-0001-7488-3318Cabrera Gumbau, Jordi Manuel\|\|\|0009-0007-3952-7589Sarmiento Guardia, IrisMartínez-Urtaza, Jaime\|\|\|0000-0001-6219-0418Rubio Lorente, Laura\|\|\|0000-0002-2088-3803Barguilla, Irene\|\|\|0000-0003-1081-4457Marcos Dauder, Ricardo\|\|\|0000-0001-7891-357XGarcía Rodríguez, Alba\|\|\|0000-0002-1175-7418Hernández Bonilla, Alba\|\|\|0000-0001-6938-1233Drosophila melanogasterMNPLsPCR16S sequencingMinIONNanoporeMicrobiotaDysbiosisMicro- and nanoplastics (MNPLs) are emerging environmental pollutants that have garnered significant attention over the past few decades due to their detrimental effects on human health through various exposure pathways. This study investigates the impact of MNPLs on gut microbiota, utilizing Drosophila melanogaster as a model organism. Drosophila was selected for its microbiota's similarities to humans and its established role as an accessible and well-characterized model system. To analyze microbiota, full-length 16S rRNA gene sequencing was performed using the Nanopore sequencing platform, enabling comprehensive profiling of the microbial populations present in the samples. As models of MNPLs, two commercial polystyrene nanoplastics (PS-NPLs, 61.20 and 415.22 nm) and one lab-made polylactic acid nanoplastic (PLA-NPLs, 463.90 nm) were selected. As a positive control, zinc oxide nanoparticles (ZnO-NPs) were used. The observed findings revealed that exposure to MNPLs induced notable alterations in gut microbiota, including a reduction in bacterial abundance and shifts in species composition. These results suggest that MNPLs exposure can lead to microbial dysbiosis and potential gut health disruptions through its interaction, either with the gut epithelial barrier or directly with the resident microorganisms. 22025-01-0120252025-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/311844https://dx.doi.org/urn:doi:10.1016/j.scitotenv.2025.179545reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2020/BP-00177Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2022/BP-00026Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2023/BP-00212European Commission https://doi.org/10.13039/501100000780 965196Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-116789RB-C43Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00731Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00526open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3118442026-06-06T12:50:31Z
dc.title.none.fl_str_mv	Nanoplastic exposure affects the intestinal microbiota of adult Drosophila flies
title	Nanoplastic exposure affects the intestinal microbiota of adult Drosophila flies
spellingShingle	Nanoplastic exposure affects the intestinal microbiota of adult Drosophila flies Rocabert Valverde, Arnau Drosophila melanogaster MNPLs PCR 16S sequencing MinION Nanopore Microbiota Dysbiosis
title_short	Nanoplastic exposure affects the intestinal microbiota of adult Drosophila flies
title_full	Nanoplastic exposure affects the intestinal microbiota of adult Drosophila flies
title_fullStr	Nanoplastic exposure affects the intestinal microbiota of adult Drosophila flies
title_full_unstemmed	Nanoplastic exposure affects the intestinal microbiota of adult Drosophila flies
title_sort	Nanoplastic exposure affects the intestinal microbiota of adult Drosophila flies
dc.creator.none.fl_str_mv	Rocabert Valverde, Arnau Martín Pérez, Joan\|\|\|0009-0006-9137-3604 Pareras Puig, Laia\|\|\|0000-0002-1381-8894 Egea, Raquel\|\|\|0000-0002-6201-4112 Alaraby, Mohamed\|\|\|0000-0001-7488-3318 Cabrera Gumbau, Jordi Manuel\|\|\|0009-0007-3952-7589 Sarmiento Guardia, Iris Martínez-Urtaza, Jaime\|\|\|0000-0001-6219-0418 Rubio Lorente, Laura\|\|\|0000-0002-2088-3803 Barguilla, Irene\|\|\|0000-0003-1081-4457 Marcos Dauder, Ricardo\|\|\|0000-0001-7891-357X García Rodríguez, Alba\|\|\|0000-0002-1175-7418 Hernández Bonilla, Alba\|\|\|0000-0001-6938-1233
author	Rocabert Valverde, Arnau
author_facet	Rocabert Valverde, Arnau Martín Pérez, Joan\|\|\|0009-0006-9137-3604 Pareras Puig, Laia\|\|\|0000-0002-1381-8894 Egea, Raquel\|\|\|0000-0002-6201-4112 Alaraby, Mohamed\|\|\|0000-0001-7488-3318 Cabrera Gumbau, Jordi Manuel\|\|\|0009-0007-3952-7589 Sarmiento Guardia, Iris Martínez-Urtaza, Jaime\|\|\|0000-0001-6219-0418 Rubio Lorente, Laura\|\|\|0000-0002-2088-3803 Barguilla, Irene\|\|\|0000-0003-1081-4457 Marcos Dauder, Ricardo\|\|\|0000-0001-7891-357X García Rodríguez, Alba\|\|\|0000-0002-1175-7418 Hernández Bonilla, Alba\|\|\|0000-0001-6938-1233
author_role	author
author2	Martín Pérez, Joan\|\|\|0009-0006-9137-3604 Pareras Puig, Laia\|\|\|0000-0002-1381-8894 Egea, Raquel\|\|\|0000-0002-6201-4112 Alaraby, Mohamed\|\|\|0000-0001-7488-3318 Cabrera Gumbau, Jordi Manuel\|\|\|0009-0007-3952-7589 Sarmiento Guardia, Iris Martínez-Urtaza, Jaime\|\|\|0000-0001-6219-0418 Rubio Lorente, Laura\|\|\|0000-0002-2088-3803 Barguilla, Irene\|\|\|0000-0003-1081-4457 Marcos Dauder, Ricardo\|\|\|0000-0001-7891-357X García Rodríguez, Alba\|\|\|0000-0002-1175-7418 Hernández Bonilla, Alba\|\|\|0000-0001-6938-1233
author2_role	author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Drosophila melanogaster MNPLs PCR 16S sequencing MinION Nanopore Microbiota Dysbiosis
topic	Drosophila melanogaster MNPLs PCR 16S sequencing MinION Nanopore Microbiota Dysbiosis
description	Micro- and nanoplastics (MNPLs) are emerging environmental pollutants that have garnered significant attention over the past few decades due to their detrimental effects on human health through various exposure pathways. This study investigates the impact of MNPLs on gut microbiota, utilizing Drosophila melanogaster as a model organism. Drosophila was selected for its microbiota's similarities to humans and its established role as an accessible and well-characterized model system. To analyze microbiota, full-length 16S rRNA gene sequencing was performed using the Nanopore sequencing platform, enabling comprehensive profiling of the microbial populations present in the samples. As models of MNPLs, two commercial polystyrene nanoplastics (PS-NPLs, 61.20 and 415.22 nm) and one lab-made polylactic acid nanoplastic (PLA-NPLs, 463.90 nm) were selected. As a positive control, zinc oxide nanoparticles (ZnO-NPs) were used. The observed findings revealed that exposure to MNPLs induced notable alterations in gut microbiota, including a reduction in bacterial abundance and shifts in species composition. These results suggest that MNPLs exposure can lead to microbial dysbiosis and potential gut health disruptions through its interaction, either with the gut epithelial barrier or directly with the resident microorganisms.
publishDate	2025
dc.date.none.fl_str_mv	2 2025-01-01 2025 2025-01-01
dc.type.none.fl_str_mv	Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://ddd.uab.cat/record/311844 https://dx.doi.org/urn:doi:10.1016/j.scitotenv.2025.179545
url	https://ddd.uab.cat/record/311844 https://dx.doi.org/urn:doi:10.1016/j.scitotenv.2025.179545
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2020/BP-00177 Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2022/BP-00026 Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2023/BP-00212 European Commission https://doi.org/10.13039/501100000780 965196 Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-116789RB-C43 Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00731 Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00526
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
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dc.source.none.fl_str_mv	reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona
instname_str	Universitat Autònoma de Barcelona
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collection	Dipòsit Digital de Documents de la UAB
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Nanoplastic exposure affects the intestinal microbiota of adult Drosophila flies

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