VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts

ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family is known to play an important role in the pathogenesis of osteoarthritis (OA), working on aggrecan degradation or altering the integrity of extracellular matrix (ECM). Thus, the main purpose of our study was to define the...

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Autores: Pérez García, Selene, Carrión Caballo, Mar, Gutiérrez Cañas, Irene, González Álvaro, Isidoro, Pérez Gomáriz, Rosa María, Juarranz Moratilla, Yasmina
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/23111
Acceso en línea:https://hdl.handle.net/20.500.14352/23111
Access Level:acceso abierto
Palabra clave:577.112
616.72-002
VIP
CRF
ADAMTS
Osteoarthritis
Synovial fibroblast
COMP
Glycosaminoglycans
Reumatología
Bioquímica (Biología)
3205.09 Reumatología
2302 Bioquímica
id ES_49ad043a1f17cc33e4374690ccff6c4e
oai_identifier_str oai:docta.ucm.es:20.500.14352/23111
network_acronym_str ES
network_name_str España
repository_id_str
spelling VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblastsPérez García, SeleneCarrión Caballo, MarGutiérrez Cañas, IreneGonzález Álvaro, IsidoroPérez Gomáriz, Rosa MaríaJuarranz Moratilla, Yasmina577.112616.72-002VIPCRFADAMTSOsteoarthritisSynovial fibroblastCOMPGlycosaminoglycansReumatologíaBioquímica (Biología)3205.09 Reumatología2302 BioquímicaADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family is known to play an important role in the pathogenesis of osteoarthritis (OA), working on aggrecan degradation or altering the integrity of extracellular matrix (ECM). Thus, the main purpose of our study was to define the role of vasoactive intestinal peptide (VIP) and corticotrophin-releasing factor (CRF), as immunoregulatory neuropeptides, on ADAMTS production in synovial fibroblasts (SF) from OA patients and healthy donors (HD). OA- and HD-SF were stimulated with pro-inflammatory mediators and treated with VIP or CRF. Both neuropeptides decreased ADAMTS-4, -5, -7 and -12 expressions, aggrecanase activity,glycosaminoglycans (GAG), and cartilage oligomeric matrix protein (COMP) degradation after stimulation with fibronectin fragments (Fn-fs) in OA-SF. After stimulation with interleukin-1b, VIP reduced ADAMTS-4 and -5, and both neuropeptides decreased ADAMTS-7 production and COMP degradation. Moreover, VIP and CRF reduced Runx2 and b-catenin activation in OA-SF. Our data suggest that the role of VIP and CRF on ADAMTS expression and cartilage degradation could be related to the OA pathology since scarce effects were produced in HD-SF. In addition,their effects might be greater when a degradation loop has been established, given that they were higher after stimulation with Fn-fs. Our results point to novel OA therapies based on the use of neuropeptides, since VIP and CRF are able to stop the first critical step, the loss of cartilageaggrecan and the ECM destabilization during joint degradationJohn WilleyUniversidad Complutense de Madrid20162016-04-0120162016-04-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/23111reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/231112026-06-02T12:44:21Z
dc.title.none.fl_str_mv VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts
title VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts
spellingShingle VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts
Pérez García, Selene
577.112
616.72-002
VIP
CRF
ADAMTS
Osteoarthritis
Synovial fibroblast
COMP
Glycosaminoglycans
Reumatología
Bioquímica (Biología)
3205.09 Reumatología
2302 Bioquímica
title_short VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts
title_full VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts
title_fullStr VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts
title_full_unstemmed VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts
title_sort VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts
dc.creator.none.fl_str_mv Pérez García, Selene
Carrión Caballo, Mar
Gutiérrez Cañas, Irene
González Álvaro, Isidoro
Pérez Gomáriz, Rosa María
Juarranz Moratilla, Yasmina
author Pérez García, Selene
author_facet Pérez García, Selene
Carrión Caballo, Mar
Gutiérrez Cañas, Irene
González Álvaro, Isidoro
Pérez Gomáriz, Rosa María
Juarranz Moratilla, Yasmina
author_role author
author2 Carrión Caballo, Mar
Gutiérrez Cañas, Irene
González Álvaro, Isidoro
Pérez Gomáriz, Rosa María
Juarranz Moratilla, Yasmina
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.112
616.72-002
VIP
CRF
ADAMTS
Osteoarthritis
Synovial fibroblast
COMP
Glycosaminoglycans
Reumatología
Bioquímica (Biología)
3205.09 Reumatología
2302 Bioquímica
topic 577.112
616.72-002
VIP
CRF
ADAMTS
Osteoarthritis
Synovial fibroblast
COMP
Glycosaminoglycans
Reumatología
Bioquímica (Biología)
3205.09 Reumatología
2302 Bioquímica
description ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family is known to play an important role in the pathogenesis of osteoarthritis (OA), working on aggrecan degradation or altering the integrity of extracellular matrix (ECM). Thus, the main purpose of our study was to define the role of vasoactive intestinal peptide (VIP) and corticotrophin-releasing factor (CRF), as immunoregulatory neuropeptides, on ADAMTS production in synovial fibroblasts (SF) from OA patients and healthy donors (HD). OA- and HD-SF were stimulated with pro-inflammatory mediators and treated with VIP or CRF. Both neuropeptides decreased ADAMTS-4, -5, -7 and -12 expressions, aggrecanase activity,glycosaminoglycans (GAG), and cartilage oligomeric matrix protein (COMP) degradation after stimulation with fibronectin fragments (Fn-fs) in OA-SF. After stimulation with interleukin-1b, VIP reduced ADAMTS-4 and -5, and both neuropeptides decreased ADAMTS-7 production and COMP degradation. Moreover, VIP and CRF reduced Runx2 and b-catenin activation in OA-SF. Our data suggest that the role of VIP and CRF on ADAMTS expression and cartilage degradation could be related to the OA pathology since scarce effects were produced in HD-SF. In addition,their effects might be greater when a degradation loop has been established, given that they were higher after stimulation with Fn-fs. Our results point to novel OA therapies based on the use of neuropeptides, since VIP and CRF are able to stop the first critical step, the loss of cartilageaggrecan and the ECM destabilization during joint degradation
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-04-01
2016
2016-04-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/23111
url https://hdl.handle.net/20.500.14352/23111
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Willey
publisher.none.fl_str_mv John Willey
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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