An investigation of the utility of plasma Cytomegalovirus (CMV) microRNA detection to predict CMV DNAemia in allogeneic hematopoietic stem cell transplant recipients

Precise identification of patients at highest risk for developing Cytomegalovirus (CMV) DNAemia may improve CMV infection management in the allogeneic hematopoietic stem cell transplantation (allo-HSCT) setting. Here, we studied thepotential use of detecting free CMV micro(mi)RNAs circulating in pla...

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Autores: Talaya, Alberto, Giménez, Estela, Pascual, María Jesús, Gago, Beatriz, Piñana, José Luis, Hernández Boluda, Juan Carlos, Vázquez, Lourdes, García, Magdalena, Serrano, David, Hernández Martín, Marta María, Albert, Eliseo, Solano, Carlos, Navarro, David
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/109957
Acceso en línea:https://hdl.handle.net/20.500.14352/109957
Access Level:acceso abierto
Palabra clave:61
Cytomegalovirus (CMV)
CMV DNAemia
Plasma CMV miRNA
Allogeneic hematopoietic stem cell transplantation (allo-HSCT)
Ciencias Biomédicas
32 Ciencias Médicas
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network_acronym_str ES
network_name_str España
repository_id_str
spelling An investigation of the utility of plasma Cytomegalovirus (CMV) microRNA detection to predict CMV DNAemia in allogeneic hematopoietic stem cell transplant recipientsTalaya, AlbertoGiménez, EstelaPascual, María JesúsGago, BeatrizPiñana, José LuisHernández Boluda, Juan CarlosVázquez, LourdesGarcía, MagdalenaSerrano, DavidHernández Martín, Marta MaríaAlbert, EliseoSolano, CarlosNavarro, David61Cytomegalovirus (CMV)CMV DNAemiaPlasma CMV miRNAAllogeneic hematopoietic stem cell transplantation (allo-HSCT)Ciencias Biomédicas32 Ciencias MédicasPrecise identification of patients at highest risk for developing Cytomegalovirus (CMV) DNAemia may improve CMV infection management in the allogeneic hematopoietic stem cell transplantation (allo-HSCT) setting. Here, we studied thepotential use of detecting free CMV micro(mi)RNAs circulating in plasma for predicting CMV DNAemia in this clinicalscenario. A total of 62 adult allo-HSCT recipients were included in this prospective observational multicenter study. PlasmaCMV DNA load was monitored using the CMV RealTime CMV PCR (Abbott Molecular, Des Plaines, IL, USA). Detection of mature CMV miRNAs in plasma drawn by days + 7, + 14 and + 30 after allo-HSCT was performed using the miScript PCR System (Qiagen, Hilden, Germany). Assays could be optimized for five out of the seven targeted CMV miRNAs: UL36-5p,US33-5p, UL148D, UL22A-5p and UL112-3p. Of the 62 patients included in the study, 42 developed a first episode of CMVDNAemia at a median of 35 days after allo-HSCT. All targeted CMV miRNA were detected early after transplantation, with CMV miRNA US33-5p and UL112-3p the most commonly found species at any time point; nevertheless, neither the detectionrate of CMV miRNAs nor their abundance allowed discrimination between patients with subsequent CMV DNAemia andthose with no CMV DNAemia. The data presented herein do not support any predictive utility of these CMV miRNAs forfirst episodes of CMV DNAemia in a cohort consisting primarily of allo-HSCT patients receiving haploidentical allografts.SPRINGERUniversidad Complutense de Madrid20192019-09-0320192019-09-03journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/109957reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1099572026-06-02T12:44:21Z
dc.title.none.fl_str_mv An investigation of the utility of plasma Cytomegalovirus (CMV) microRNA detection to predict CMV DNAemia in allogeneic hematopoietic stem cell transplant recipients
title An investigation of the utility of plasma Cytomegalovirus (CMV) microRNA detection to predict CMV DNAemia in allogeneic hematopoietic stem cell transplant recipients
spellingShingle An investigation of the utility of plasma Cytomegalovirus (CMV) microRNA detection to predict CMV DNAemia in allogeneic hematopoietic stem cell transplant recipients
Talaya, Alberto
61
Cytomegalovirus (CMV)
CMV DNAemia
Plasma CMV miRNA
Allogeneic hematopoietic stem cell transplantation (allo-HSCT)
Ciencias Biomédicas
32 Ciencias Médicas
title_short An investigation of the utility of plasma Cytomegalovirus (CMV) microRNA detection to predict CMV DNAemia in allogeneic hematopoietic stem cell transplant recipients
title_full An investigation of the utility of plasma Cytomegalovirus (CMV) microRNA detection to predict CMV DNAemia in allogeneic hematopoietic stem cell transplant recipients
title_fullStr An investigation of the utility of plasma Cytomegalovirus (CMV) microRNA detection to predict CMV DNAemia in allogeneic hematopoietic stem cell transplant recipients
title_full_unstemmed An investigation of the utility of plasma Cytomegalovirus (CMV) microRNA detection to predict CMV DNAemia in allogeneic hematopoietic stem cell transplant recipients
title_sort An investigation of the utility of plasma Cytomegalovirus (CMV) microRNA detection to predict CMV DNAemia in allogeneic hematopoietic stem cell transplant recipients
dc.creator.none.fl_str_mv Talaya, Alberto
Giménez, Estela
Pascual, María Jesús
Gago, Beatriz
Piñana, José Luis
Hernández Boluda, Juan Carlos
Vázquez, Lourdes
García, Magdalena
Serrano, David
Hernández Martín, Marta María
Albert, Eliseo
Solano, Carlos
Navarro, David
author Talaya, Alberto
author_facet Talaya, Alberto
Giménez, Estela
Pascual, María Jesús
Gago, Beatriz
Piñana, José Luis
Hernández Boluda, Juan Carlos
Vázquez, Lourdes
García, Magdalena
Serrano, David
Hernández Martín, Marta María
Albert, Eliseo
Solano, Carlos
Navarro, David
author_role author
author2 Giménez, Estela
Pascual, María Jesús
Gago, Beatriz
Piñana, José Luis
Hernández Boluda, Juan Carlos
Vázquez, Lourdes
García, Magdalena
Serrano, David
Hernández Martín, Marta María
Albert, Eliseo
Solano, Carlos
Navarro, David
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 61
Cytomegalovirus (CMV)
CMV DNAemia
Plasma CMV miRNA
Allogeneic hematopoietic stem cell transplantation (allo-HSCT)
Ciencias Biomédicas
32 Ciencias Médicas
topic 61
Cytomegalovirus (CMV)
CMV DNAemia
Plasma CMV miRNA
Allogeneic hematopoietic stem cell transplantation (allo-HSCT)
Ciencias Biomédicas
32 Ciencias Médicas
description Precise identification of patients at highest risk for developing Cytomegalovirus (CMV) DNAemia may improve CMV infection management in the allogeneic hematopoietic stem cell transplantation (allo-HSCT) setting. Here, we studied thepotential use of detecting free CMV micro(mi)RNAs circulating in plasma for predicting CMV DNAemia in this clinicalscenario. A total of 62 adult allo-HSCT recipients were included in this prospective observational multicenter study. PlasmaCMV DNA load was monitored using the CMV RealTime CMV PCR (Abbott Molecular, Des Plaines, IL, USA). Detection of mature CMV miRNAs in plasma drawn by days + 7, + 14 and + 30 after allo-HSCT was performed using the miScript PCR System (Qiagen, Hilden, Germany). Assays could be optimized for five out of the seven targeted CMV miRNAs: UL36-5p,US33-5p, UL148D, UL22A-5p and UL112-3p. Of the 62 patients included in the study, 42 developed a first episode of CMVDNAemia at a median of 35 days after allo-HSCT. All targeted CMV miRNA were detected early after transplantation, with CMV miRNA US33-5p and UL112-3p the most commonly found species at any time point; nevertheless, neither the detectionrate of CMV miRNAs nor their abundance allowed discrimination between patients with subsequent CMV DNAemia andthose with no CMV DNAemia. The data presented herein do not support any predictive utility of these CMV miRNAs forfirst episodes of CMV DNAemia in a cohort consisting primarily of allo-HSCT patients receiving haploidentical allografts.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-09-03
2019
2019-09-03
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/109957
url https://hdl.handle.net/20.500.14352/109957
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SPRINGER
publisher.none.fl_str_mv SPRINGER
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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