New methologies for studying carbohydrate-protein interacions by nuclear magnetic resonance

Carbohydrates (glycan, sugars, saccharides) play a key role in life and disease. Their wide presence in different processes comprises from the structural and energetic function to the basis of the molecular recognition of physiological and pathogenic events. Indeed, they are involved in many biologi...

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Detalhes bibliográficos
Autor: Fernández de Toro Ronda, Beatriz
Formato: tesis doctoral
Fecha de publicación:2021
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/11274
Acesso em linha:https://hdl.handle.net/20.500.14352/11274
Access Level:acceso abierto
Palavra-chave:577.114(043.2)
Hidratos de carbono
Carbohydrates
Dietética y nutrición (Farmacia)
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spelling New methologies for studying carbohydrate-protein interacions by nuclear magnetic resonanceNuevas metodologías para el estudio de la interacción carbohidrato-proteína empleando la resonancia magnética nuclearFernández de Toro Ronda, Beatriz577.114(043.2)Hidratos de carbonoCarbohydratesDietética y nutrición (Farmacia)Carbohydrates (glycan, sugars, saccharides) play a key role in life and disease. Their wide presence in different processes comprises from the structural and energetic function to the basis of the molecular recognition of physiological and pathogenic events. Indeed, they are involved in many biological events of Paramount importance (i.e. cell-cell, cell-matrix or cell-molecules interactions). Therefore, the study of the molecular recognition events in which sugars are involved is a key task for understanding these interactions, and the structural elucidation and the conformational studies of saccharides are crucial to unravel their interaction mechanisms as well as the biological implications of these events. However, the inherent flexibility of carbohydrates precludes their crystallization, hampering the study by X-ray diffraction. In contrast, Nuclear Magnetic Resonance (NMR) permits to work in solution, mimicking the physiological conditions, and thus provides information on sugar geometries and dynamics. Therefore, NMR spectroscopy is the preferred technique for the structural characterization of carbohydrates, becoming a powerful tool for the understanding of the molecular processes in which carbohydrates are involved. In this context, two biological systems have been studied in this Thesis with the aim to understand the conformation and dynamics of different saccharides and glycomimetics in solution and to unravel key features of their molecular recognition processes with their receptors…Universidad Complutense de MadridCañada Vicinay, Francisco JavierCanales Mayordomo, María ÁngelesJiménez Barbero, JesúsUniversidad Complutense de Madrid20212021-02-0120212021-02-01doctoral thesishttp://purl.org/coar/resource_type/c_db06info:eu-repo/semantics/doctoralThesisapplication/pdfhttps://hdl.handle.net/20.500.14352/11274reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/112742026-06-02T12:44:21Z
dc.title.none.fl_str_mv New methologies for studying carbohydrate-protein interacions by nuclear magnetic resonance
Nuevas metodologías para el estudio de la interacción carbohidrato-proteína empleando la resonancia magnética nuclear
title New methologies for studying carbohydrate-protein interacions by nuclear magnetic resonance
spellingShingle New methologies for studying carbohydrate-protein interacions by nuclear magnetic resonance
Fernández de Toro Ronda, Beatriz
577.114(043.2)
Hidratos de carbono
Carbohydrates
Dietética y nutrición (Farmacia)
title_short New methologies for studying carbohydrate-protein interacions by nuclear magnetic resonance
title_full New methologies for studying carbohydrate-protein interacions by nuclear magnetic resonance
title_fullStr New methologies for studying carbohydrate-protein interacions by nuclear magnetic resonance
title_full_unstemmed New methologies for studying carbohydrate-protein interacions by nuclear magnetic resonance
title_sort New methologies for studying carbohydrate-protein interacions by nuclear magnetic resonance
dc.creator.none.fl_str_mv Fernández de Toro Ronda, Beatriz
author Fernández de Toro Ronda, Beatriz
author_facet Fernández de Toro Ronda, Beatriz
author_role author
dc.contributor.none.fl_str_mv Cañada Vicinay, Francisco Javier
Canales Mayordomo, María Ángeles
Jiménez Barbero, Jesús
Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.114(043.2)
Hidratos de carbono
Carbohydrates
Dietética y nutrición (Farmacia)
topic 577.114(043.2)
Hidratos de carbono
Carbohydrates
Dietética y nutrición (Farmacia)
description Carbohydrates (glycan, sugars, saccharides) play a key role in life and disease. Their wide presence in different processes comprises from the structural and energetic function to the basis of the molecular recognition of physiological and pathogenic events. Indeed, they are involved in many biological events of Paramount importance (i.e. cell-cell, cell-matrix or cell-molecules interactions). Therefore, the study of the molecular recognition events in which sugars are involved is a key task for understanding these interactions, and the structural elucidation and the conformational studies of saccharides are crucial to unravel their interaction mechanisms as well as the biological implications of these events. However, the inherent flexibility of carbohydrates precludes their crystallization, hampering the study by X-ray diffraction. In contrast, Nuclear Magnetic Resonance (NMR) permits to work in solution, mimicking the physiological conditions, and thus provides information on sugar geometries and dynamics. Therefore, NMR spectroscopy is the preferred technique for the structural characterization of carbohydrates, becoming a powerful tool for the understanding of the molecular processes in which carbohydrates are involved. In this context, two biological systems have been studied in this Thesis with the aim to understand the conformation and dynamics of different saccharides and glycomimetics in solution and to unravel key features of their molecular recognition processes with their receptors…
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-02-01
2021
2021-02-01
dc.type.none.fl_str_mv doctoral thesis
http://purl.org/coar/resource_type/c_db06
dc.type.openaire.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/11274
url https://hdl.handle.net/20.500.14352/11274
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Complutense de Madrid
publisher.none.fl_str_mv Universidad Complutense de Madrid
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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