New methologies for studying carbohydrate-protein interacions by nuclear magnetic resonance
Carbohydrates (glycan, sugars, saccharides) play a key role in life and disease. Their wide presence in different processes comprises from the structural and energetic function to the basis of the molecular recognition of physiological and pathogenic events. Indeed, they are involved in many biologi...
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| Tipo de recurso: | tesis doctoral |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/11274 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/11274 |
| Access Level: | acceso abierto |
| Palabra clave: | 577.114(043.2) Hidratos de carbono Carbohydrates Dietética y nutrición (Farmacia) |
| Sumario: | Carbohydrates (glycan, sugars, saccharides) play a key role in life and disease. Their wide presence in different processes comprises from the structural and energetic function to the basis of the molecular recognition of physiological and pathogenic events. Indeed, they are involved in many biological events of Paramount importance (i.e. cell-cell, cell-matrix or cell-molecules interactions). Therefore, the study of the molecular recognition events in which sugars are involved is a key task for understanding these interactions, and the structural elucidation and the conformational studies of saccharides are crucial to unravel their interaction mechanisms as well as the biological implications of these events. However, the inherent flexibility of carbohydrates precludes their crystallization, hampering the study by X-ray diffraction. In contrast, Nuclear Magnetic Resonance (NMR) permits to work in solution, mimicking the physiological conditions, and thus provides information on sugar geometries and dynamics. Therefore, NMR spectroscopy is the preferred technique for the structural characterization of carbohydrates, becoming a powerful tool for the understanding of the molecular processes in which carbohydrates are involved. In this context, two biological systems have been studied in this Thesis with the aim to understand the conformation and dynamics of different saccharides and glycomimetics in solution and to unravel key features of their molecular recognition processes with their receptors… |
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