Analysis of interaction between dendriplexes and bovine serum albumin.

[EN] Dendrimers are new nanotechnological carriers for gene delivery. Short oligodeoxynucleotides (ODNs) are a new class of antisense therapy drugs for cancer and infectious or metabolic diseases. The interactions between short oligodeoxynucleotides (GEM91, CTCTCGCACCCATCTCTCTCCTTCT; SREV, TCGTCGCTG...

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Detalles Bibliográficos
Autores: Shcharbin, Dzmitry, Pedziwiatr, Elzbieta, Chonco, Louis, Bermejo Martín, Jesús Francisco, Ortega, Paula, de la Mata, F Javier, Eritja, Ramon, Gómez, Rafael, Klajnert, Barbara, Bryszewska, Maria, Muñoz-Fernandez, Ma Angeles
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/161089
Acceso en línea:http://hdl.handle.net/10366/161089
Access Level:acceso embargado
Palabra clave:Base Sequence
DNA Primers
Dendrimers
Serum Albumin, Bovine
Spectrometry, Fluorescence
Serum Albumin
secuencia de bases
albúmina sérica
dendrímeros
cebadores de ADN
id ES_4746aafae2a1cff57be3ed72d1c5ec8a
oai_identifier_str oai:gredos.usal.es:10366/161089
network_acronym_str ES
network_name_str España
repository_id_str
spelling Analysis of interaction between dendriplexes and bovine serum albumin.Shcharbin, DzmitryPedziwiatr, ElzbietaChonco, LouisBermejo Martín, Jesús FranciscoOrtega, Paulade la Mata, F JavierEritja, RamonGómez, RafaelKlajnert, BarbaraBryszewska, MariaMuñoz-Fernandez, Ma AngelesBase SequenceDNA PrimersDendrimersSerum Albumin, BovineSpectrometry, FluorescenceDendrimersBase SequenceDNA PrimersSerum Albuminsecuencia de basesalbúmina séricadendrímeroscebadores de ADN[EN] Dendrimers are new nanotechnological carriers for gene delivery. Short oligodeoxynucleotides (ODNs) are a new class of antisense therapy drugs for cancer and infectious or metabolic diseases. The interactions between short oligodeoxynucleotides (GEM91, CTCTCGCACCCATCTCTCTCCTTCT; SREV, TCGTCGCTGTCTCCGCTTCTTCCTGCCA; unlabeled or fluorescein-labeled), novel water-soluble carbosilane dendrimers, and bovine serum albumin were studied by fluorescence and gel electrophoresis. The molar ratios of the dendrimer/ODN dendriplexes ranged from 4 to 7. The efficiency of formation and stability of the dendriplexes depended on electrostatic interactions between the dendrimer and the ODNs. Dendriplex formation significantly decreased the interactions between ODNs and albumin. Thus, the formation of dendriplexes between carbosilane dendrimers and ODNs may improve ODN delivery.info202420242007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/161089reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésCTQ2005-00795/BQUSAF-2004-06778SAF-2003-09209RIS G03/173PI061479FIPSE (Grant 36514/05)Grant BQU2004-02048BM04-52-0CM04/00136Marie Curie International Incoming Fellowship (Grant 510018)Kasa Mianowski FundAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/embargoedAccessoai:gredos.usal.es:10366/1610892026-06-07T06:28:51Z
dc.title.none.fl_str_mv Analysis of interaction between dendriplexes and bovine serum albumin.
title Analysis of interaction between dendriplexes and bovine serum albumin.
spellingShingle Analysis of interaction between dendriplexes and bovine serum albumin.
Shcharbin, Dzmitry
Base Sequence
DNA Primers
Dendrimers
Serum Albumin, Bovine
Spectrometry, Fluorescence
Dendrimers
Base Sequence
DNA Primers
Serum Albumin
secuencia de bases
albúmina sérica
dendrímeros
cebadores de ADN
title_short Analysis of interaction between dendriplexes and bovine serum albumin.
title_full Analysis of interaction between dendriplexes and bovine serum albumin.
title_fullStr Analysis of interaction between dendriplexes and bovine serum albumin.
title_full_unstemmed Analysis of interaction between dendriplexes and bovine serum albumin.
title_sort Analysis of interaction between dendriplexes and bovine serum albumin.
dc.creator.none.fl_str_mv Shcharbin, Dzmitry
Pedziwiatr, Elzbieta
Chonco, Louis
Bermejo Martín, Jesús Francisco
Ortega, Paula
de la Mata, F Javier
Eritja, Ramon
Gómez, Rafael
Klajnert, Barbara
Bryszewska, Maria
Muñoz-Fernandez, Ma Angeles
author Shcharbin, Dzmitry
author_facet Shcharbin, Dzmitry
Pedziwiatr, Elzbieta
Chonco, Louis
Bermejo Martín, Jesús Francisco
Ortega, Paula
de la Mata, F Javier
Eritja, Ramon
Gómez, Rafael
Klajnert, Barbara
Bryszewska, Maria
Muñoz-Fernandez, Ma Angeles
author_role author
author2 Pedziwiatr, Elzbieta
Chonco, Louis
Bermejo Martín, Jesús Francisco
Ortega, Paula
de la Mata, F Javier
Eritja, Ramon
Gómez, Rafael
Klajnert, Barbara
Bryszewska, Maria
Muñoz-Fernandez, Ma Angeles
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Base Sequence
DNA Primers
Dendrimers
Serum Albumin, Bovine
Spectrometry, Fluorescence
Dendrimers
Base Sequence
DNA Primers
Serum Albumin
secuencia de bases
albúmina sérica
dendrímeros
cebadores de ADN
topic Base Sequence
DNA Primers
Dendrimers
Serum Albumin, Bovine
Spectrometry, Fluorescence
Dendrimers
Base Sequence
DNA Primers
Serum Albumin
secuencia de bases
albúmina sérica
dendrímeros
cebadores de ADN
description [EN] Dendrimers are new nanotechnological carriers for gene delivery. Short oligodeoxynucleotides (ODNs) are a new class of antisense therapy drugs for cancer and infectious or metabolic diseases. The interactions between short oligodeoxynucleotides (GEM91, CTCTCGCACCCATCTCTCTCCTTCT; SREV, TCGTCGCTGTCTCCGCTTCTTCCTGCCA; unlabeled or fluorescein-labeled), novel water-soluble carbosilane dendrimers, and bovine serum albumin were studied by fluorescence and gel electrophoresis. The molar ratios of the dendrimer/ODN dendriplexes ranged from 4 to 7. The efficiency of formation and stability of the dendriplexes depended on electrostatic interactions between the dendrimer and the ODNs. Dendriplex formation significantly decreased the interactions between ODNs and albumin. Thus, the formation of dendriplexes between carbosilane dendrimers and ODNs may improve ODN delivery.
publishDate 2007
dc.date.none.fl_str_mv 2007
2024
2024
info
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/161089
url http://hdl.handle.net/10366/161089
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv CTQ2005-00795/BQU
SAF-2004-06778
SAF-2003-09209
RIS G03/173
PI061479
FIPSE (Grant 36514/05)
Grant BQU2004-02048
BM04-52-0
CM04/00136
Marie Curie International Incoming Fellowship (Grant 510018)
Kasa Mianowski Fund
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv embargoedAccess
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407283615629312
score 15.81155

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