Direct mass spectrometry-based approaches in metabolomics
Metabolomics is one of the newest Omics technologies concerned with the identification and quantification of small molecules in a high-throughput manner. Considering the number of different types of metabolites present in a wide dynamic range of concentrations in any single living system, actual ana...
| Authors: | , , , |
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| Format: | other |
| Status: | Versión aceptada para publicación |
| Publication Date: | 2014 |
| Country: | España |
| Institution: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repository: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/99917 |
| Online Access: | http://hdl.handle.net/10261/99917 |
| Access Level: | Open access |
| Keyword: | DESI SIMS MALDI Ambient MS DART Imaging MS NIMS Mass spectrometry |
| Summary: | Metabolomics is one of the newest Omics technologies concerned with the identification and quantification of small molecules in a high-throughput manner. Considering the number of different types of metabolites present in a wide dynamic range of concentrations in any single living system, actual analytical technologies can still only capture a part of the metabolome. Currently, MS-based approaches yield a higher sensitivity than NMR when analyzing minimal amounts of complex mixtures. Most MS-based approaches in metabolomics involve a physical/chemical purification/fractionation prior to MS analysis, to avoid sample matrix effects, at the expense of low high-throughput performance. In the quest to achieve the maximum high-throughput production of metabolite information in the largest possible number of samples, an extensive array of direct ionization or desorption/ionization techniques have been developed and combined. In the this chapter, an overview of the main desorption/ionization techniques coupled with MS applied to direct metabolite profiling or fingerprinting is presented. © 2014 Elsevier B.V. |
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