Spatial distribution of calcium sparks determines their ability to induce afterdepolarizations in human atrial myocytes

Analysis of the spatio-temporal distribution of calcium sparks showed a preferential increase in sparks near the sarcolemma in atrial myocytes from patients with atrial fibrillation (AF), linked to higher ryanodine receptor (RyR2) phosphorylation at s2808 and lower calsequestrin-2 levels. Mathematic...

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Detalles Bibliográficos
Autores: Tarifa, Carmen, Vallmitjana, Alexander, Jiménez Sábado, Verónica, Marchena, Miquel, Llach, Anna, Herraiz Martínez, Adela, Godoy-Marín, Héctor, Nolla-Colomer, Carme, Ginel, Antonino, Viñolas, Xavier, Montiel, José, Ciruela Alférez, Francisco, Echebarria, Blas, Benitez, Raúl, Cinca, Juan, Hove-Madsen, Leif
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/202167
Acceso en línea:https://hdl.handle.net/2445/202167
Access Level:acceso abierto
Palabra clave:Fibril·lació auricular
Cèl·lules musculars
Canals de calci
Atrial fibrillation
Muscle cells
Calcium channels
Descripción
Sumario:Analysis of the spatio-temporal distribution of calcium sparks showed a preferential increase in sparks near the sarcolemma in atrial myocytes from patients with atrial fibrillation (AF), linked to higher ryanodine receptor (RyR2) phosphorylation at s2808 and lower calsequestrin-2 levels. Mathematical modeling, incorporating modulation of RyR2 gating, showed that only the observed combinations of RyR2 phosphorylation and calsequestrin-2 levels can account for the spatio-temporal distribution of sparks in patients with and without AF. Furthermore, we demonstrate that preferential calcium release near the sarcolemma is key to a higher incidence and amplitude of afterdepolarizations in atrial myocytes from patients with AF