Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer

Extracellular vesicles (EV) are emerging structures with promising properties for intercellular communication. In addition, the characterization of EV in biofluids is an attractive source of non-invasive diagnostic, prognostic and predictive biomarkers. Here we show that urinary EV (uEV) from prosta...

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Authors: Royo, Felix, Zúñiga García, Patricia, Torrano, Verónica, Loizaga Iriarte, Ana, Sánchez Mosquera, Pilar, Ugalde-Olano, Aitziber, González, Esperanza, Cortazar, Ana Rosa, Palomo, Laura, Fernández-Ruiz, Sonia, Lacasa-Viscasillas, Isabel, Berdasco, María, Sutherland, James David, Barrio, Rosa, Zabala Letona, Amaia, Martín-Martín, Natalia, Arruabarrena-Aristorena, Amaia, Valcarcel-Jimenez, Lorea, Caro Maldonado, Alfredo, Gonzalez-Tampan, Jorge, Cachi-Fuentes, Guido, Esteller, Manel, 1968-, Aransay, Ana M., Unda Urzaiz, Miguel, Falcón Pérez, Juan Manuel, Carracedo, Arkaitz
Format: article
Status:Published version
Publication Date:2016
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/114213
Online Access:https://hdl.handle.net/2445/114213
Access Level:Open access
Keyword:Càncer de pròstata
Malalties de la pròstata
Marcadors tumorals
Marcadors bioquímics
Anàlisi d'orina
Prostate cancer
Prostatic diseases
Tumor markers
Biochemical markers
Urine analysis
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repository_id_str
spelling Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancerRoyo, FelixZúñiga García, PatriciaTorrano, VerónicaLoizaga Iriarte, AnaSánchez Mosquera, PilarUgalde-Olano, AitziberGonzález, EsperanzaCortazar, Ana RosaPalomo, LauraFernández-Ruiz, SoniaLacasa-Viscasillas, IsabelBerdasco, MaríaSutherland, James DavidBarrio, RosaZabala Letona, AmaiaMartín-Martín, NataliaArruabarrena-Aristorena, AmaiaValcarcel-Jimenez, LoreaCaro Maldonado, AlfredoGonzalez-Tampan, JorgeCachi-Fuentes, GuidoEsteller, Manel, 1968-Aransay, Ana M.Unda Urzaiz, MiguelFalcón Pérez, Juan ManuelCarracedo, ArkaitzCàncer de pròstataMalalties de la pròstataMarcadors tumoralsMarcadors bioquímicsAnàlisi d'orinaProstate cancerProstatic diseasesTumor markersBiochemical markersUrine analysisExtracellular vesicles (EV) are emerging structures with promising properties for intercellular communication. In addition, the characterization of EV in biofluids is an attractive source of non-invasive diagnostic, prognostic and predictive biomarkers. Here we show that urinary EV (uEV) from prostate cancer (PCa) patients exhibit genuine and differential physical and biological properties compared to benign prostate hyperplasia (BPH). Importantly, transcriptomics characterization of uEVs led us to define the decreased abundance of Cadherin 3, type 1 (CDH3) transcript in uEV from PCa patients. Tissue and cell line analysis strongly suggested that the status of CDH3 in uEVs is a distal reflection of changes in the expression of this cadherin in the prostate tumor. CDH3 was negatively regulated at the genomic, transcriptional, and epigenetic level in PCa. Our results reveal that uEVs could represent a non-invasive tool to inform about the molecular alterations in PCa.Impact Journals2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/114213Articles publicats en revistes (Ciències Fisiològiques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.18632/oncotarget.6899Oncotarget, 2016, vol. 7, num. 6, p. 6835-6846https://doi.org/10.18632/oncotarget.6899info:eu-repo/grantAgreement/EC/FP7/336343cc-by (c) Royo, Felix et al., 2016http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1142132026-05-27T06:46:51Z
dc.title.none.fl_str_mv Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer
title Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer
spellingShingle Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer
Royo, Felix
Càncer de pròstata
Malalties de la pròstata
Marcadors tumorals
Marcadors bioquímics
Anàlisi d'orina
Prostate cancer
Prostatic diseases
Tumor markers
Biochemical markers
Urine analysis
title_short Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer
title_full Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer
title_fullStr Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer
title_full_unstemmed Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer
title_sort Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer
dc.creator.none.fl_str_mv Royo, Felix
Zúñiga García, Patricia
Torrano, Verónica
Loizaga Iriarte, Ana
Sánchez Mosquera, Pilar
Ugalde-Olano, Aitziber
González, Esperanza
Cortazar, Ana Rosa
Palomo, Laura
Fernández-Ruiz, Sonia
Lacasa-Viscasillas, Isabel
Berdasco, María
Sutherland, James David
Barrio, Rosa
Zabala Letona, Amaia
Martín-Martín, Natalia
Arruabarrena-Aristorena, Amaia
Valcarcel-Jimenez, Lorea
Caro Maldonado, Alfredo
Gonzalez-Tampan, Jorge
Cachi-Fuentes, Guido
Esteller, Manel, 1968-
Aransay, Ana M.
Unda Urzaiz, Miguel
Falcón Pérez, Juan Manuel
Carracedo, Arkaitz
author Royo, Felix
author_facet Royo, Felix
Zúñiga García, Patricia
Torrano, Verónica
Loizaga Iriarte, Ana
Sánchez Mosquera, Pilar
Ugalde-Olano, Aitziber
González, Esperanza
Cortazar, Ana Rosa
Palomo, Laura
Fernández-Ruiz, Sonia
Lacasa-Viscasillas, Isabel
Berdasco, María
Sutherland, James David
Barrio, Rosa
Zabala Letona, Amaia
Martín-Martín, Natalia
Arruabarrena-Aristorena, Amaia
Valcarcel-Jimenez, Lorea
Caro Maldonado, Alfredo
Gonzalez-Tampan, Jorge
Cachi-Fuentes, Guido
Esteller, Manel, 1968-
Aransay, Ana M.
Unda Urzaiz, Miguel
Falcón Pérez, Juan Manuel
Carracedo, Arkaitz
author_role author
author2 Zúñiga García, Patricia
Torrano, Verónica
Loizaga Iriarte, Ana
Sánchez Mosquera, Pilar
Ugalde-Olano, Aitziber
González, Esperanza
Cortazar, Ana Rosa
Palomo, Laura
Fernández-Ruiz, Sonia
Lacasa-Viscasillas, Isabel
Berdasco, María
Sutherland, James David
Barrio, Rosa
Zabala Letona, Amaia
Martín-Martín, Natalia
Arruabarrena-Aristorena, Amaia
Valcarcel-Jimenez, Lorea
Caro Maldonado, Alfredo
Gonzalez-Tampan, Jorge
Cachi-Fuentes, Guido
Esteller, Manel, 1968-
Aransay, Ana M.
Unda Urzaiz, Miguel
Falcón Pérez, Juan Manuel
Carracedo, Arkaitz
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Càncer de pròstata
Malalties de la pròstata
Marcadors tumorals
Marcadors bioquímics
Anàlisi d'orina
Prostate cancer
Prostatic diseases
Tumor markers
Biochemical markers
Urine analysis
topic Càncer de pròstata
Malalties de la pròstata
Marcadors tumorals
Marcadors bioquímics
Anàlisi d'orina
Prostate cancer
Prostatic diseases
Tumor markers
Biochemical markers
Urine analysis
description Extracellular vesicles (EV) are emerging structures with promising properties for intercellular communication. In addition, the characterization of EV in biofluids is an attractive source of non-invasive diagnostic, prognostic and predictive biomarkers. Here we show that urinary EV (uEV) from prostate cancer (PCa) patients exhibit genuine and differential physical and biological properties compared to benign prostate hyperplasia (BPH). Importantly, transcriptomics characterization of uEVs led us to define the decreased abundance of Cadherin 3, type 1 (CDH3) transcript in uEV from PCa patients. Tissue and cell line analysis strongly suggested that the status of CDH3 in uEVs is a distal reflection of changes in the expression of this cadherin in the prostate tumor. CDH3 was negatively regulated at the genomic, transcriptional, and epigenetic level in PCa. Our results reveal that uEVs could represent a non-invasive tool to inform about the molecular alterations in PCa.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/114213
url https://hdl.handle.net/2445/114213
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.18632/oncotarget.6899
Oncotarget, 2016, vol. 7, num. 6, p. 6835-6846
https://doi.org/10.18632/oncotarget.6899
info:eu-repo/grantAgreement/EC/FP7/336343
dc.rights.none.fl_str_mv cc-by (c) Royo, Felix et al., 2016
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Royo, Felix et al., 2016
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Impact Journals
publisher.none.fl_str_mv Impact Journals
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Fisiològiques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407196170682368
score 15,300719

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