Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer

Extracellular vesicles (EV) are emerging structures with promising properties for intercellular communication. In addition, the characterization of EV in biofluids is an attractive source of non-invasive diagnostic, prognostic and predictive biomarkers. Here we show that urinary EV (uEV) from prosta...

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Detalles Bibliográficos
Autores: Royo, Felix, Zúñiga García, Patricia, Torrano, Verónica, Loizaga Iriarte, Ana, Sánchez Mosquera, Pilar, Ugalde-Olano, Aitziber, González, Esperanza, Cortazar, Ana Rosa, Palomo, Laura, Fernández-Ruiz, Sonia, Lacasa-Viscasillas, Isabel, Berdasco, María, Sutherland, James David, Barrio, Rosa, Zabala Letona, Amaia, Martín-Martín, Natalia, Arruabarrena-Aristorena, Amaia, Valcarcel-Jimenez, Lorea, Caro Maldonado, Alfredo, Gonzalez-Tampan, Jorge, Cachi-Fuentes, Guido, Esteller, Manel, Aransay, Ana M., Unda Urzaiz, Miguel, Falcón Pérez, Juan Manuel, Carracedo, Arkaitz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/114213
Acceso en línea:https://hdl.handle.net/2445/114213
Access Level:acceso abierto
Palabra clave:Càncer de pròstata
Malalties de la pròstata
Marcadors tumorals
Marcadors bioquímics
Anàlisi d'orina
Prostate cancer
Prostatic diseases
Tumor markers
Biochemical markers
Urine analysis
Descripción
Sumario:Extracellular vesicles (EV) are emerging structures with promising properties for intercellular communication. In addition, the characterization of EV in biofluids is an attractive source of non-invasive diagnostic, prognostic and predictive biomarkers. Here we show that urinary EV (uEV) from prostate cancer (PCa) patients exhibit genuine and differential physical and biological properties compared to benign prostate hyperplasia (BPH). Importantly, transcriptomics characterization of uEVs led us to define the decreased abundance of Cadherin 3, type 1 (CDH3) transcript in uEV from PCa patients. Tissue and cell line analysis strongly suggested that the status of CDH3 in uEVs is a distal reflection of changes in the expression of this cadherin in the prostate tumor. CDH3 was negatively regulated at the genomic, transcriptional, and epigenetic level in PCa. Our results reveal that uEVs could represent a non-invasive tool to inform about the molecular alterations in PCa.

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