Genipin-crosslinked pectin hydrogels: A dual strategy for enhanced 3D printability and stability
The development of polysaccharide-based biomaterial inks with tailored physicochemical properties and dynamic responsiveness currently attracts a lot of scientific interest. Pectin, a natural and biocompatible polysaccharide, shows great promise for a wide range of biomedical applications, but its l...
| Autores: | , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2026 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/416104 |
| Acceso en línea: | http://hdl.handle.net/10261/416104 https://api.elsevier.com/content/abstract/scopus_id/105022157717 |
| Access Level: | acceso abierto |
| Palabra clave: | 3d extrusion printing Biomaterial inks Chemical crosslinking Hydrogels Pectin pH-responsive |
| Sumario: | The development of polysaccharide-based biomaterial inks with tailored physicochemical properties and dynamic responsiveness currently attracts a lot of scientific interest. Pectin, a natural and biocompatible polysaccharide, shows great promise for a wide range of biomedical applications, but its limited mechanical strength and printability constrain its application in 3D extrusion bioprinting. This study introduces a strategy to overcome these drawbacks via amine modification and genipin crosslinking, producing hydrogels with improved mechanical stability under physiological conditions. Pectin hydrogels formed at various amine:genipin ratios (1:0.6 to 1:3) exhibited elastic moduli values in the range of 3-5 KPa under physiological conditions, with crosslinking efficiency reaching a plateau. Biomaterial inks comprising crosslinked pectin particles were dispersed in a reactive aqueous pectin solution, thus enabling precise 3D extrusion of scaffolds with up to 7 layers, maintaining structural integrity post-lyophilization. The 3D printed pectin hydrogels showed enhanced porosity and swelling kinetics, particularly in response to pH changes, while maintaining high hydrolytic stability and non-cytotoxicity. This approach provides a promising platform for developing dynamic, pectin-based bioinks in bio applications requiring of extrusion based additive manufacturing techniques. |
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