Characterization of a native polysaccharide hapten from Brucella melitensis

The 13C nuclear magnetic resonance spectrum of Brucella melitensis native polysaccharide hapten proved to be very similar to that generated by the O-specific chain (PS) isolated from B. melitensis lipopolysaccharide; that is, to a linear polymer in which the repeating unit is composed of five N-form...

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Bibliographic Details
Authors: Moreno, E. (Edgardo)|||/items/11186877-6440-423f-a77a-c63ec45c54b0, Mayer, H. (Hubert)|||/items/e13aa0d7-8b63-4148-9830-b7220c9c94af, Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b
Format: article
Publication Date:1987
Country:España
Institution:Universidad de Navarra
Repository:Dadun. Depósito Académico Digital de la Universidad de Navarra
Language:English
OAI Identifier:oai:dadun.unav.edu:10171/29522
Online Access:https://hdl.handle.net/10171/29522
Access Level:Open access
Keyword:Antigens, bacterial isolation and purification
Brucella immunology
Polysaccharides
Bacterial immunology
Description
Summary:The 13C nuclear magnetic resonance spectrum of Brucella melitensis native polysaccharide hapten proved to be very similar to that generated by the O-specific chain (PS) isolated from B. melitensis lipopolysaccharide; that is, to a linear polymer in which the repeating unit is composed of five N-formylperosaminyl residues, one of them being substituted at position C-3 and the other four at position C-2. The serological analysis suggests that the so-called A determinant is present solely in Brucella abortus PS, the M determinant is only in B. melitensis PS, and the extensive cross-reaction observed is due to a determinant shared by both polysaccharides.