Application of pressurized liquid extractions to obtain bioactive compounds from tuber aestivum and terfezia claveryi

A PLE (pressurized liquid extraction) method was adjusted following a full-factorial experimental design to obtain bioactive-enriched fractions from Tuber aestivum and Terfezia claveryi. Temperature, time and solvent (water, ethanol and ethanol–water 1:1) parameters were investi-gated. The response...

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Detalles Bibliográficos
Autores: Tejedor Calvo, Eva, García-Barreda, Sergi, Sánchez, Sergio, Morte, Asunción, Siles Sánchez, María de las Nieves, Soler Rivas, Cristina, Santoyo Díez, Susana, Marco, Pedro
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/706805
Acceso en línea:http://hdl.handle.net/10486/706805
https://dx.doi.org/10.3390/foods11030298
Access Level:acceso abierto
Palabra clave:Antioxidants
Enzymes
Immunomodulation
Response surface methodology
Terfezia claveryi
Tuber aestivum
Ciencia y Tecnología de Alimentos
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spelling Application of pressurized liquid extractions to obtain bioactive compounds from tuber aestivum and terfezia claveryiTejedor Calvo, EvaGarcía-Barreda, SergiSánchez, SergioMorte, AsunciónSiles Sánchez, María de las NievesSoler Rivas, CristinaSantoyo Díez, SusanaMarco, PedroAntioxidantsEnzymesImmunomodulationResponse surface methodologyTerfezia claveryiTuber aestivumCiencia y Tecnología de AlimentosA PLE (pressurized liquid extraction) method was adjusted following a full-factorial experimental design to obtain bioactive-enriched fractions from Tuber aestivum and Terfezia claveryi. Temperature, time and solvent (water, ethanol and ethanol–water 1:1) parameters were investi-gated. The response variables investigated were: obtained yield and the levels of total carbohydrate (compounds, β-glucans, chitin, proteins, phenolic compounds and sterols). Principal component analysis indicated water solvent and high temperatures as more adequate parameters to extract polysaccharide-rich fractions (up to 68% of content), whereas ethanol was more suitable to extract fungal sterols (up to 12.5% of content). The fractions obtained at optimal conditions (16.7 MPa, 180◦ C, 30 min) were able to protect Caco2 cells from free radical exposure, acting as antioxidants, and were able to reduce secretion of pro-inflammatory cytokines in vitro: IL-6 (50%), and TNFα (80% only T. claveryi ethanol extract), as well as reduce high inhibitory activity (T. aestivum IC50: 9.44 mG/mL)MDPIDepartamento de Química Física AplicadaFacultad de Ciencias20222022-01-23research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/706805https://dx.doi.org/10.3390/foods11030298reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7068052026-06-23T12:46:27Z
dc.title.none.fl_str_mv Application of pressurized liquid extractions to obtain bioactive compounds from tuber aestivum and terfezia claveryi
title Application of pressurized liquid extractions to obtain bioactive compounds from tuber aestivum and terfezia claveryi
spellingShingle Application of pressurized liquid extractions to obtain bioactive compounds from tuber aestivum and terfezia claveryi
Tejedor Calvo, Eva
Antioxidants
Enzymes
Immunomodulation
Response surface methodology
Terfezia claveryi
Tuber aestivum
Ciencia y Tecnología de Alimentos
title_short Application of pressurized liquid extractions to obtain bioactive compounds from tuber aestivum and terfezia claveryi
title_full Application of pressurized liquid extractions to obtain bioactive compounds from tuber aestivum and terfezia claveryi
title_fullStr Application of pressurized liquid extractions to obtain bioactive compounds from tuber aestivum and terfezia claveryi
title_full_unstemmed Application of pressurized liquid extractions to obtain bioactive compounds from tuber aestivum and terfezia claveryi
title_sort Application of pressurized liquid extractions to obtain bioactive compounds from tuber aestivum and terfezia claveryi
dc.creator.none.fl_str_mv Tejedor Calvo, Eva
García-Barreda, Sergi
Sánchez, Sergio
Morte, Asunción
Siles Sánchez, María de las Nieves
Soler Rivas, Cristina
Santoyo Díez, Susana
Marco, Pedro
author Tejedor Calvo, Eva
author_facet Tejedor Calvo, Eva
García-Barreda, Sergi
Sánchez, Sergio
Morte, Asunción
Siles Sánchez, María de las Nieves
Soler Rivas, Cristina
Santoyo Díez, Susana
Marco, Pedro
author_role author
author2 García-Barreda, Sergi
Sánchez, Sergio
Morte, Asunción
Siles Sánchez, María de las Nieves
Soler Rivas, Cristina
Santoyo Díez, Susana
Marco, Pedro
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Química Física Aplicada
Facultad de Ciencias
dc.subject.none.fl_str_mv Antioxidants
Enzymes
Immunomodulation
Response surface methodology
Terfezia claveryi
Tuber aestivum
Ciencia y Tecnología de Alimentos
topic Antioxidants
Enzymes
Immunomodulation
Response surface methodology
Terfezia claveryi
Tuber aestivum
Ciencia y Tecnología de Alimentos
description A PLE (pressurized liquid extraction) method was adjusted following a full-factorial experimental design to obtain bioactive-enriched fractions from Tuber aestivum and Terfezia claveryi. Temperature, time and solvent (water, ethanol and ethanol–water 1:1) parameters were investi-gated. The response variables investigated were: obtained yield and the levels of total carbohydrate (compounds, β-glucans, chitin, proteins, phenolic compounds and sterols). Principal component analysis indicated water solvent and high temperatures as more adequate parameters to extract polysaccharide-rich fractions (up to 68% of content), whereas ethanol was more suitable to extract fungal sterols (up to 12.5% of content). The fractions obtained at optimal conditions (16.7 MPa, 180◦ C, 30 min) were able to protect Caco2 cells from free radical exposure, acting as antioxidants, and were able to reduce secretion of pro-inflammatory cytokines in vitro: IL-6 (50%), and TNFα (80% only T. claveryi ethanol extract), as well as reduce high inhibitory activity (T. aestivum IC50: 9.44 mG/mL)
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-23
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/706805
https://dx.doi.org/10.3390/foods11030298
url http://hdl.handle.net/10486/706805
https://dx.doi.org/10.3390/foods11030298
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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