Characterization of Pediococcus ethanolidurans CUPV141: A b-D-glucan- and Heteropolysaccharide-Producing Bacterium

Pediococcus ethanolidurans CUPV141 is an exopolysaccharide (EPS)-producing lactic acid bacterium, first isolated from Basque Country cider (Spain). Physicochemical analysis of the EPS synthesized by the bacterium revealed that CUPV141 produces mostly a homopolysaccharide (HoPS), characterized as a 2...

Descripción completa

Detalles Bibliográficos
Autores: Llamas Arriba, María Goretti, Pérez Ramos, Adrián, Puertas González, Ana Isabel, López García, Paloma, Dueñas Chasco, María Teresa, Prieto Orzanco, Alicia
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/30697
Acceso en línea:http://hdl.handle.net/10810/30697
Access Level:acceso abierto
Palabra clave:pediococcus ethanolidurans
beta-glucan
gtf
heteropolysaccharides
priming-glycosyltransferase
plasmid
adhesion
priming glycosyltransferase
streptococcus-thermophilus
lactobacillus-suebicus
functional-analysis
lactococcus-lactis
stress tolerance
caco-2 cells
id ES_43e62cae9aade3e7d695ca09ffba5582
oai_identifier_str oai:addi.ehu.eus:10810/30697
network_acronym_str ES
network_name_str España
repository_id_str
spelling Characterization of Pediococcus ethanolidurans CUPV141: A b-D-glucan- and Heteropolysaccharide-Producing BacteriumLlamas Arriba, María GorettiPérez Ramos, AdriánPuertas González, Ana IsabelLópez García, PalomaDueñas Chasco, María TeresaPrieto Orzanco, Aliciapediococcus ethanoliduransbeta-glucangtfheteropolysaccharidespriming-glycosyltransferaseplasmidadhesionpriming glycosyltransferasestreptococcus-thermophiluslactobacillus-suebicusfunctional-analysislactococcus-lactisstress tolerancecaco-2 cellsPediococcus ethanolidurans CUPV141 is an exopolysaccharide (EPS)-producing lactic acid bacterium, first isolated from Basque Country cider (Spain). Physicochemical analysis of the EPS synthesized by the bacterium revealed that CUPV141 produces mostly a homopolysaccharide (HoPS), characterized as a 2-substituted (1,3)-beta-D-glucan, together with a small quantity of a heteropolysaccharide (HePS) composed of glucose, galactose, glucosamine, and glycerol-3-phosphate, this being the first Pediococcus strain described to produce this kind of polymer. On the contrary, an isogenic strain CUPV141NR, generated by chemical mutagenesis of CUPV141, produced the HePS as the main extracellular polysaccharide and a barely detectable amount of 2-substituted (1,3)-beta-D-glucan. This HoPS is synthesized by the transmembrane GTF glycosyltransferase (GTF), encoded by the gtf gene, which has been previously reported to be located in the pPP2 plasmid of the Pediococcus parvulus 2.6 strain. Southern blot hybridization revealed that in CUPV141 the gtf gene is located in a plasmid designated as pPE3, whose molecular mass (34.4 kbp) is different from that of pPP2 (24.5 kbp). Analysis of the influence of the EPS on the ability of the producing bacteria to adhere to the eukaryotic Caco-2 cells revealed higher affinity for the human enterocytes of CUPV141NR compared to that of CUPV141. This result indicates that, in contrast to the 2.6 strain, the presence of the HoPS does not potentiate the binding ability of P. ethanolidurans. Moreover, it supports that the phosphate-containing bacterial HePS improved the interaction between P. ethanolidurans and the eukaryotic cells.This work was supported by a grant from the Department of Environment, Territorial Planification, Agriculture and Fishing and the project no. IT 866-13 from the Basque Government and by the Spanish Ministry of Science and Innovation (AGL 2015-65010-C3-1-R).Frontiers Media201920192018info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/30697reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.frontiersin.org/articles/10.3389/fmicb.2018.02041/fullinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/© 2018 Llamas-Arriba, Pérez-Ramos, Puertas, López, Dueñas and Prieto. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.Atribución 3.0 Españaoai:addi.ehu.eus:10810/306972026-06-18T09:23:17Z
dc.title.none.fl_str_mv Characterization of Pediococcus ethanolidurans CUPV141: A b-D-glucan- and Heteropolysaccharide-Producing Bacterium
title Characterization of Pediococcus ethanolidurans CUPV141: A b-D-glucan- and Heteropolysaccharide-Producing Bacterium
spellingShingle Characterization of Pediococcus ethanolidurans CUPV141: A b-D-glucan- and Heteropolysaccharide-Producing Bacterium
Llamas Arriba, María Goretti
pediococcus ethanolidurans
beta-glucan
gtf
heteropolysaccharides
priming-glycosyltransferase
plasmid
adhesion
priming glycosyltransferase
streptococcus-thermophilus
lactobacillus-suebicus
functional-analysis
lactococcus-lactis
stress tolerance
caco-2 cells
title_short Characterization of Pediococcus ethanolidurans CUPV141: A b-D-glucan- and Heteropolysaccharide-Producing Bacterium
title_full Characterization of Pediococcus ethanolidurans CUPV141: A b-D-glucan- and Heteropolysaccharide-Producing Bacterium
title_fullStr Characterization of Pediococcus ethanolidurans CUPV141: A b-D-glucan- and Heteropolysaccharide-Producing Bacterium
title_full_unstemmed Characterization of Pediococcus ethanolidurans CUPV141: A b-D-glucan- and Heteropolysaccharide-Producing Bacterium
title_sort Characterization of Pediococcus ethanolidurans CUPV141: A b-D-glucan- and Heteropolysaccharide-Producing Bacterium
dc.creator.none.fl_str_mv Llamas Arriba, María Goretti
Pérez Ramos, Adrián
Puertas González, Ana Isabel
López García, Paloma
Dueñas Chasco, María Teresa
Prieto Orzanco, Alicia
author Llamas Arriba, María Goretti
author_facet Llamas Arriba, María Goretti
Pérez Ramos, Adrián
Puertas González, Ana Isabel
López García, Paloma
Dueñas Chasco, María Teresa
Prieto Orzanco, Alicia
author_role author
author2 Pérez Ramos, Adrián
Puertas González, Ana Isabel
López García, Paloma
Dueñas Chasco, María Teresa
Prieto Orzanco, Alicia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv pediococcus ethanolidurans
beta-glucan
gtf
heteropolysaccharides
priming-glycosyltransferase
plasmid
adhesion
priming glycosyltransferase
streptococcus-thermophilus
lactobacillus-suebicus
functional-analysis
lactococcus-lactis
stress tolerance
caco-2 cells
topic pediococcus ethanolidurans
beta-glucan
gtf
heteropolysaccharides
priming-glycosyltransferase
plasmid
adhesion
priming glycosyltransferase
streptococcus-thermophilus
lactobacillus-suebicus
functional-analysis
lactococcus-lactis
stress tolerance
caco-2 cells
description Pediococcus ethanolidurans CUPV141 is an exopolysaccharide (EPS)-producing lactic acid bacterium, first isolated from Basque Country cider (Spain). Physicochemical analysis of the EPS synthesized by the bacterium revealed that CUPV141 produces mostly a homopolysaccharide (HoPS), characterized as a 2-substituted (1,3)-beta-D-glucan, together with a small quantity of a heteropolysaccharide (HePS) composed of glucose, galactose, glucosamine, and glycerol-3-phosphate, this being the first Pediococcus strain described to produce this kind of polymer. On the contrary, an isogenic strain CUPV141NR, generated by chemical mutagenesis of CUPV141, produced the HePS as the main extracellular polysaccharide and a barely detectable amount of 2-substituted (1,3)-beta-D-glucan. This HoPS is synthesized by the transmembrane GTF glycosyltransferase (GTF), encoded by the gtf gene, which has been previously reported to be located in the pPP2 plasmid of the Pediococcus parvulus 2.6 strain. Southern blot hybridization revealed that in CUPV141 the gtf gene is located in a plasmid designated as pPE3, whose molecular mass (34.4 kbp) is different from that of pPP2 (24.5 kbp). Analysis of the influence of the EPS on the ability of the producing bacteria to adhere to the eukaryotic Caco-2 cells revealed higher affinity for the human enterocytes of CUPV141NR compared to that of CUPV141. This result indicates that, in contrast to the 2.6 strain, the presence of the HoPS does not potentiate the binding ability of P. ethanolidurans. Moreover, it supports that the phosphate-containing bacterial HePS improved the interaction between P. ethanolidurans and the eukaryotic cells.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/30697
url http://hdl.handle.net/10810/30697
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.frontiersin.org/articles/10.3389/fmicb.2018.02041/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407055926788096
score 15.301603

Similares en LA Referencia