Water partial pressure X-ray photoelectron spectroscopy study of the conformation of fibrinogen on silanized hydrophilic/hydrophobic surfaces

Near ambient pressure X-ray photoelectron spectroscopy (NAP-XPS) allows the study of the conformational state of adsorbed proteins on surfaces at water partial pressures of a few mbar. In the present study, we used two organosilanes to prepare hydrophilic and hydrophobic surfaces. For the NAP-XPS st...

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Autores: Fernández-Alonso, Francisco Javier, Calvo, Rodrigo, Sanz Calderón, Aida, Villar-García, Ignacio J., Saiz, Fernan, Hernando-Pérez, Mercedes, Pérez Dieste, Virginia, Manso Silván, Miguel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/417374
Acceso en línea:http://hdl.handle.net/10261/417374
https://api.elsevier.com/content/abstract/scopus_id/105009277436
Access Level:acceso abierto
Palabra clave:Conformation
Fibrinogen
H2O partial pressure
Hydrophilic-hydrophobic
NAP-XPS
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spelling Water partial pressure X-ray photoelectron spectroscopy study of the conformation of fibrinogen on silanized hydrophilic/hydrophobic surfacesFernández-Alonso, Francisco JavierCalvo, RodrigoSanz Calderón, AidaVillar-García, Ignacio J.Saiz, FernanHernando-Pérez, MercedesPérez Dieste, VirginiaManso Silván, MiguelConformationFibrinogenH2O partial pressureHydrophilic-hydrophobicNAP-XPSNear ambient pressure X-ray photoelectron spectroscopy (NAP-XPS) allows the study of the conformational state of adsorbed proteins on surfaces at water partial pressures of a few mbar. In the present study, we used two organosilanes to prepare hydrophilic and hydrophobic surfaces. For the NAP-XPS study, human fibrinogen, a sorbent serum protein with conformational dependent function, was adsorbed on contrasting surfaces, studied at 2 mbar H<inf>2</inf>O vapor pressure and compared with analysis at ultrahigh vacuum (UHV). Two different excitation energies were used to gain in-depth sensitivity. The C 1 s core level was fitted, and the components correlated with the presence of surface-exposed hydrophobic or hydrophilic moieties. The mode of analysis significantly affects the data on the conformation of fibrinogen on hydrophilic surfaces, showing surface-exposed (more intense) hydrophobic cues in the H<inf>2</inf>O NAP mode than in the UHV mode. Furthermore, the intensity of the C[sbnd]H peak exhibits the greatest variability in intensity, being more surface segregated on hydrophilic surfaces than on hydrophobic ones. The latter statement is sustained only for H<inf>2</inf>O NAP conditions, with no significant differences observed in the UHV mode. The work envisages greater sensitivity for forthcoming analyses of adsorbed proteins and other biomolecules by using water partial pressure XPS mode.The current research was funded through grants PID2020–112770RB-C22, PID2022–141080OB-C22, CNS2022–135285, PID2023–147200OB 100, PID2023–151371OB-C22, PID2023–151078OB-I00 and RyC2021–030929-I by MCIN/AEI/10.13039/501100011033 and European Union NextGenerationEU/PRTR. Additional funding from SI3/PJI/ 2021–00216 by Comunidad de Madrid and UAM is also acknowledged. FJFA acknowledges the Formación de Profesorado Universitario program, ref. FPU22/04365. Time allocated at ALBA synchrotron through 2020094555-BL24-CIRCE experiment is greatly appreciated.Peer reviewedElsevierMinisterio de Ciencia, Innovación y Universidades (España)European CommissionComunidad de MadridFernández-Alonso, Francisco Javier [0000-0002-7943-9328]Sanz Calderón, Aida [0000-0001-6185-653X]Saiz, Fernan [0000-0001-8565-8044]Manso Silván, Miguel [0000-0002-5063-1607]202620262025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/417374https://api.elsevier.com/content/abstract/scopus_id/105009277436reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112770RB-C22info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-141080OB-C22info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-147200OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-151371OB-C22info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-151078OB-I00https://doi.org/10.1016/j.surfin.2025.106964Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4173742026-05-22T06:33:51Z
dc.title.none.fl_str_mv Water partial pressure X-ray photoelectron spectroscopy study of the conformation of fibrinogen on silanized hydrophilic/hydrophobic surfaces
title Water partial pressure X-ray photoelectron spectroscopy study of the conformation of fibrinogen on silanized hydrophilic/hydrophobic surfaces
spellingShingle Water partial pressure X-ray photoelectron spectroscopy study of the conformation of fibrinogen on silanized hydrophilic/hydrophobic surfaces
Fernández-Alonso, Francisco Javier
Conformation
Fibrinogen
H2O partial pressure
Hydrophilic-hydrophobic
NAP-XPS
title_short Water partial pressure X-ray photoelectron spectroscopy study of the conformation of fibrinogen on silanized hydrophilic/hydrophobic surfaces
title_full Water partial pressure X-ray photoelectron spectroscopy study of the conformation of fibrinogen on silanized hydrophilic/hydrophobic surfaces
title_fullStr Water partial pressure X-ray photoelectron spectroscopy study of the conformation of fibrinogen on silanized hydrophilic/hydrophobic surfaces
title_full_unstemmed Water partial pressure X-ray photoelectron spectroscopy study of the conformation of fibrinogen on silanized hydrophilic/hydrophobic surfaces
title_sort Water partial pressure X-ray photoelectron spectroscopy study of the conformation of fibrinogen on silanized hydrophilic/hydrophobic surfaces
dc.creator.none.fl_str_mv Fernández-Alonso, Francisco Javier
Calvo, Rodrigo
Sanz Calderón, Aida
Villar-García, Ignacio J.
Saiz, Fernan
Hernando-Pérez, Mercedes
Pérez Dieste, Virginia
Manso Silván, Miguel
author Fernández-Alonso, Francisco Javier
author_facet Fernández-Alonso, Francisco Javier
Calvo, Rodrigo
Sanz Calderón, Aida
Villar-García, Ignacio J.
Saiz, Fernan
Hernando-Pérez, Mercedes
Pérez Dieste, Virginia
Manso Silván, Miguel
author_role author
author2 Calvo, Rodrigo
Sanz Calderón, Aida
Villar-García, Ignacio J.
Saiz, Fernan
Hernando-Pérez, Mercedes
Pérez Dieste, Virginia
Manso Silván, Miguel
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Comunidad de Madrid
Fernández-Alonso, Francisco Javier [0000-0002-7943-9328]
Sanz Calderón, Aida [0000-0001-6185-653X]
Saiz, Fernan [0000-0001-8565-8044]
Manso Silván, Miguel [0000-0002-5063-1607]
dc.subject.none.fl_str_mv Conformation
Fibrinogen
H2O partial pressure
Hydrophilic-hydrophobic
NAP-XPS
topic Conformation
Fibrinogen
H2O partial pressure
Hydrophilic-hydrophobic
NAP-XPS
description Near ambient pressure X-ray photoelectron spectroscopy (NAP-XPS) allows the study of the conformational state of adsorbed proteins on surfaces at water partial pressures of a few mbar. In the present study, we used two organosilanes to prepare hydrophilic and hydrophobic surfaces. For the NAP-XPS study, human fibrinogen, a sorbent serum protein with conformational dependent function, was adsorbed on contrasting surfaces, studied at 2 mbar H<inf>2</inf>O vapor pressure and compared with analysis at ultrahigh vacuum (UHV). Two different excitation energies were used to gain in-depth sensitivity. The C 1 s core level was fitted, and the components correlated with the presence of surface-exposed hydrophobic or hydrophilic moieties. The mode of analysis significantly affects the data on the conformation of fibrinogen on hydrophilic surfaces, showing surface-exposed (more intense) hydrophobic cues in the H<inf>2</inf>O NAP mode than in the UHV mode. Furthermore, the intensity of the C[sbnd]H peak exhibits the greatest variability in intensity, being more surface segregated on hydrophilic surfaces than on hydrophobic ones. The latter statement is sustained only for H<inf>2</inf>O NAP conditions, with no significant differences observed in the UHV mode. The work envisages greater sensitivity for forthcoming analyses of adsorbed proteins and other biomolecules by using water partial pressure XPS mode.
publishDate 2025
dc.date.none.fl_str_mv 2025
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/417374
https://api.elsevier.com/content/abstract/scopus_id/105009277436
url http://hdl.handle.net/10261/417374
https://api.elsevier.com/content/abstract/scopus_id/105009277436
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112770RB-C22
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-141080OB-C22
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-147200OB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-151371OB-C22
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-151078OB-I00
https://doi.org/10.1016/j.surfin.2025.106964
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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