Class III peroxidases (POD) in Pepper (Capsicum annuum L.): genome-wide identification and regulation during nitric oxide (NO)-influenced fruit ripening

The class III peroxidases (PODs) catalyze the oxidation of several substrates coupled to the reduction of HO to water, and play important roles in diverse plant processes. The POD family members have been well-studied in several plant species, but little information is available on sweet pepper frui...

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Autores: González-Gordo, Salvador, Muñoz-Vargas, María A., Palma Martínez, José Manuel, Corpas, Francisco J.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/331984
Acesso em linha:http://hdl.handle.net/10261/331984
Access Level:acceso abierto
Palavra-chave:Fruit ripening
Nitric oxide
Nitration
Peroxidase
Pepper
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spelling Class III peroxidases (POD) in Pepper (Capsicum annuum L.): genome-wide identification and regulation during nitric oxide (NO)-influenced fruit ripeningGonzález-Gordo, SalvadorMuñoz-Vargas, María A.Palma Martínez, José ManuelCorpas, Francisco J.Fruit ripeningNitric oxideNitrationPeroxidasePepperThe class III peroxidases (PODs) catalyze the oxidation of several substrates coupled to the reduction of HO to water, and play important roles in diverse plant processes. The POD family members have been well-studied in several plant species, but little information is available on sweet pepper fruit physiology. Based on the existing pepper genome, a total of 75 CaPOD genes have been identified, but only 10 genes were found in the fruit transcriptome (RNA-Seq). The time-course expression analysis of these genes showed that two were upregulated during fruit ripening, seven were downregulated, and one gene was unaffected. Furthermore, nitric oxide (NO) treatment triggered the upregulation of two CaPOD genes whereas the others were unaffected. Non-denaturing PAGE and in-gel activity staining allowed identifying four CaPOD isozymes (CaPOD I-CaPOD IV) which were differentially modulated during ripening and by NO. In vitro analyses of green fruit samples with peroxynitrite, NO donors, and reducing agents triggered about 100% inhibition of CaPOD IV. These data support the modulation of POD at gene and activity levels, which is in agreement with the nitro-oxidative metabolism of pepper fruit during ripening, and suggest that POD IV is a target for nitration and reducing events that lead to its inhibition.Our research is supported by a European Regional Development Fund cofinanced grants from the Ministry of Science and Innovation (PID2019-103924GB-I00), the AEI (10.13039/501100011033) and Junta de Andalucía (P18-FR-1359), Spain.Multidisciplinary Digital Publishing InstituteEuropean CommissionMinisterio de Ciencia e Innovación (España)Junta de AndalucíaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/331984reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-103924GB-I00http://dx.doi.org/10.3390/antiox12051013Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3319842026-05-22T06:33:51Z
dc.title.none.fl_str_mv Class III peroxidases (POD) in Pepper (Capsicum annuum L.): genome-wide identification and regulation during nitric oxide (NO)-influenced fruit ripening
title Class III peroxidases (POD) in Pepper (Capsicum annuum L.): genome-wide identification and regulation during nitric oxide (NO)-influenced fruit ripening
spellingShingle Class III peroxidases (POD) in Pepper (Capsicum annuum L.): genome-wide identification and regulation during nitric oxide (NO)-influenced fruit ripening
González-Gordo, Salvador
Fruit ripening
Nitric oxide
Nitration
Peroxidase
Pepper
title_short Class III peroxidases (POD) in Pepper (Capsicum annuum L.): genome-wide identification and regulation during nitric oxide (NO)-influenced fruit ripening
title_full Class III peroxidases (POD) in Pepper (Capsicum annuum L.): genome-wide identification and regulation during nitric oxide (NO)-influenced fruit ripening
title_fullStr Class III peroxidases (POD) in Pepper (Capsicum annuum L.): genome-wide identification and regulation during nitric oxide (NO)-influenced fruit ripening
title_full_unstemmed Class III peroxidases (POD) in Pepper (Capsicum annuum L.): genome-wide identification and regulation during nitric oxide (NO)-influenced fruit ripening
title_sort Class III peroxidases (POD) in Pepper (Capsicum annuum L.): genome-wide identification and regulation during nitric oxide (NO)-influenced fruit ripening
dc.creator.none.fl_str_mv González-Gordo, Salvador
Muñoz-Vargas, María A.
Palma Martínez, José Manuel
Corpas, Francisco J.
author González-Gordo, Salvador
author_facet González-Gordo, Salvador
Muñoz-Vargas, María A.
Palma Martínez, José Manuel
Corpas, Francisco J.
author_role author
author2 Muñoz-Vargas, María A.
Palma Martínez, José Manuel
Corpas, Francisco J.
author2_role author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia e Innovación (España)
Junta de Andalucía
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fruit ripening
Nitric oxide
Nitration
Peroxidase
Pepper
topic Fruit ripening
Nitric oxide
Nitration
Peroxidase
Pepper
description The class III peroxidases (PODs) catalyze the oxidation of several substrates coupled to the reduction of HO to water, and play important roles in diverse plant processes. The POD family members have been well-studied in several plant species, but little information is available on sweet pepper fruit physiology. Based on the existing pepper genome, a total of 75 CaPOD genes have been identified, but only 10 genes were found in the fruit transcriptome (RNA-Seq). The time-course expression analysis of these genes showed that two were upregulated during fruit ripening, seven were downregulated, and one gene was unaffected. Furthermore, nitric oxide (NO) treatment triggered the upregulation of two CaPOD genes whereas the others were unaffected. Non-denaturing PAGE and in-gel activity staining allowed identifying four CaPOD isozymes (CaPOD I-CaPOD IV) which were differentially modulated during ripening and by NO. In vitro analyses of green fruit samples with peroxynitrite, NO donors, and reducing agents triggered about 100% inhibition of CaPOD IV. These data support the modulation of POD at gene and activity levels, which is in agreement with the nitro-oxidative metabolism of pepper fruit during ripening, and suggest that POD IV is a target for nitration and reducing events that lead to its inhibition.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/331984
url http://hdl.handle.net/10261/331984
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-103924GB-I00
http://dx.doi.org/10.3390/antiox12051013
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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