On the influence of structural and chemical properties on the elastic modulus of woven bone under healing

Introduction: Woven bone, a heterogeneous and temporary tissue in bone regeneration, is remodeled by osteoblastic and osteoclastic activity and shaped by mechanical stress to restore healthy tissue properties. Characterizing this tissue at different length scales is crucial for developing micromecha...

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Autores: Blázquez-Carmona, Pablo, Mora Macías, Juan, Pajares Vicente, Antonia, Mármol, Álvaro, Reina Romo, Esther
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad de Huelva (UHU)
Repositorio:Arias Montano. Repositorio Institucional de la Universidad de Huelva
Idioma:inglés
OAI Identifier:oai:ariasmontano.uhu.es:10272/24738
Acceso en línea:https://hdl.handle.net/10272/24738
Access Level:acceso abierto
Palabra clave:Woven bone
Nanoindentation
Micro-CT
Raman sprectroscopy
Composition
Distraction osteogenesis
Elastic modulus
Power law
33 Ciencias Tecnológicas
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spelling On the influence of structural and chemical properties on the elastic modulus of woven bone under healingBlázquez-Carmona, PabloMora Macías, JuanPajares Vicente, AntoniaMármol, ÁlvaroReina Romo, EstherWoven boneNanoindentationMicro-CTRaman sprectroscopyCompositionDistraction osteogenesisElastic modulusPower law33 Ciencias TecnológicasIntroduction: Woven bone, a heterogeneous and temporary tissue in bone regeneration, is remodeled by osteoblastic and osteoclastic activity and shaped by mechanical stress to restore healthy tissue properties. Characterizing this tissue at different length scales is crucial for developing micromechanical models that optimize mechanical parameters, thereby controlling regeneration and preventing non-unions. Methods: This study examines the temporal evolution of the mechanical properties of bone distraction callus using nanoindentation, ash analysis, micro-CT for trabecular microarchitecture, and Raman spectroscopy for mineral quality. It also establishes single- and two-parameter power laws based on experimental data to predict tissue-level and bulk mechanical properties. Results: At the macro-scale, the tissue exhibited a considerable increase in bone fraction, controlled by the widening of trabeculae. The Raman mineral-to-matrix ratios increased to cortical levels during regeneration, but the local elastic modulus remained lower. During healing, the tissue underwent changes in ash fraction and in the percentages of Calcium and Phosphorus. Six statistically significant power laws were identified based on the ash fraction, bone fraction, and chemical and Raman parameters. Discussion: The microarchitecture of woven bone plays a more significant role than its chemical composition in determining the apparent elastic modulus of the tissue. Raman parameters were demonstrated to provide more significant power laws correlations with the micro-scale elastic modulus than mineral content from ash analysis.Frontiers Media20242024-10-0120242024-10-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10272/24738reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelvainstname:Universidad de Huelva (UHU)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ariasmontano.uhu.es:10272/247382026-06-02T14:58:11Z
dc.title.none.fl_str_mv On the influence of structural and chemical properties on the elastic modulus of woven bone under healing
title On the influence of structural and chemical properties on the elastic modulus of woven bone under healing
spellingShingle On the influence of structural and chemical properties on the elastic modulus of woven bone under healing
Blázquez-Carmona, Pablo
Woven bone
Nanoindentation
Micro-CT
Raman sprectroscopy
Composition
Distraction osteogenesis
Elastic modulus
Power law
33 Ciencias Tecnológicas
title_short On the influence of structural and chemical properties on the elastic modulus of woven bone under healing
title_full On the influence of structural and chemical properties on the elastic modulus of woven bone under healing
title_fullStr On the influence of structural and chemical properties on the elastic modulus of woven bone under healing
title_full_unstemmed On the influence of structural and chemical properties on the elastic modulus of woven bone under healing
title_sort On the influence of structural and chemical properties on the elastic modulus of woven bone under healing
dc.creator.none.fl_str_mv Blázquez-Carmona, Pablo
Mora Macías, Juan
Pajares Vicente, Antonia
Mármol, Álvaro
Reina Romo, Esther
author Blázquez-Carmona, Pablo
author_facet Blázquez-Carmona, Pablo
Mora Macías, Juan
Pajares Vicente, Antonia
Mármol, Álvaro
Reina Romo, Esther
author_role author
author2 Mora Macías, Juan
Pajares Vicente, Antonia
Mármol, Álvaro
Reina Romo, Esther
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Woven bone
Nanoindentation
Micro-CT
Raman sprectroscopy
Composition
Distraction osteogenesis
Elastic modulus
Power law
33 Ciencias Tecnológicas
topic Woven bone
Nanoindentation
Micro-CT
Raman sprectroscopy
Composition
Distraction osteogenesis
Elastic modulus
Power law
33 Ciencias Tecnológicas
description Introduction: Woven bone, a heterogeneous and temporary tissue in bone regeneration, is remodeled by osteoblastic and osteoclastic activity and shaped by mechanical stress to restore healthy tissue properties. Characterizing this tissue at different length scales is crucial for developing micromechanical models that optimize mechanical parameters, thereby controlling regeneration and preventing non-unions. Methods: This study examines the temporal evolution of the mechanical properties of bone distraction callus using nanoindentation, ash analysis, micro-CT for trabecular microarchitecture, and Raman spectroscopy for mineral quality. It also establishes single- and two-parameter power laws based on experimental data to predict tissue-level and bulk mechanical properties. Results: At the macro-scale, the tissue exhibited a considerable increase in bone fraction, controlled by the widening of trabeculae. The Raman mineral-to-matrix ratios increased to cortical levels during regeneration, but the local elastic modulus remained lower. During healing, the tissue underwent changes in ash fraction and in the percentages of Calcium and Phosphorus. Six statistically significant power laws were identified based on the ash fraction, bone fraction, and chemical and Raman parameters. Discussion: The microarchitecture of woven bone plays a more significant role than its chemical composition in determining the apparent elastic modulus of the tissue. Raman parameters were demonstrated to provide more significant power laws correlations with the micro-scale elastic modulus than mineral content from ash analysis.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-10-01
2024
2024-10-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10272/24738
url https://hdl.handle.net/10272/24738
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelva
instname:Universidad de Huelva (UHU)
instname_str Universidad de Huelva (UHU)
reponame_str Arias Montano. Repositorio Institucional de la Universidad de Huelva
collection Arias Montano. Repositorio Institucional de la Universidad de Huelva
repository.name.fl_str_mv
repository.mail.fl_str_mv
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