El papel de las proteínas DELLA en el procesamiento alternativo de mensajeros

DELLA proteins, the master repressors of the gibberellin (GA) signaling pathway, are thought to regulate gene expression by mechanisms other than their interaction with bona fide transcription factors. In particular, current work in our lab shows that DELLA and their atypical interactors prefoldins...

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Detalles Bibliográficos
Autor: Calleja Cabrera, Julián
Tipo de recurso: tesis de maestría
Fecha de publicación:2018
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:español
OAI Identifier:oai:riunet.upv.es:10251/97802
Acceso en línea:https://riunet.upv.es/handle/10251/97802
Access Level:acceso abierto
Palabra clave:DELLA
Sm
LSM
Prefoldin
Arabidopsis
Alternative splicing
Gibberellins
Prefoldina
Splicing alternativo
BIOQUIMICA Y BIOLOGIA MOLECULAR
Máster Universitario en Biotecnología Molecular y Celular de Plantas-Màster Universitari en Biotecnologia Molecular i Cel·lular de Plantes
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spelling El papel de las proteínas DELLA en el procesamiento alternativo de mensajerosThe role of ‘DELLA’ proteins in alternative splicingCalleja Cabrera, JuliánDELLASmLSMPrefoldinArabidopsisAlternative splicingGibberellinsPrefoldinaSplicing alternativoBIOQUIMICA Y BIOLOGIA MOLECULARMáster Universitario en Biotecnología Molecular y Celular de Plantas-Màster Universitari en Biotecnologia Molecular i Cel·lular de PlantesDELLA proteins, the master repressors of the gibberellin (GA) signaling pathway, are thought to regulate gene expression by mechanisms other than their interaction with bona fide transcription factors. In particular, current work in our lab shows that DELLA and their atypical interactors prefoldins are able to interact with and regulate a number of proteins that are part of the basal transcriptional machinery, regulating the RNA polymerase II activity. Among the novel interactors being studied are proteins involved in mRNA splicing. In this TFM, we have investigated the participation of DELLA and prefoldin in alternative splicing by analyzing protein-protein interaction between these proteins and Sm/LSm, which form heteroheptameric complexes and are an essential part of the spliceosome. We have also advanced in obtaining resources to test genetic interactions between della and sm/lsm mutants. In addition, some alternative splicing events affected by DELLA activity identified in a transcriptomic analysis by RNA-seq, have been validated by semiquantitative RT-PCR. Finally, it has been shown that GA signaling alters the Sm and LSm protein levels.Universitat Politècnica de ValènciaAlabadí Diego, DavidEsteve Bruna, DavidInstituto Universitario Mixto de Biología Molecular y Celular de PlantasRepositorio Institucional de la Universitat Politècnica de València Riunet20182018-02-1320182018-01-24master thesishttp://purl.org/coar/resource_type/c_bdccinfo:eu-repo/semantics/masterThesisapplication/pdfhttps://riunet.upv.es/handle/10251/97802reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)Españolspaopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/978022026-06-13T07:49:27Z
dc.title.none.fl_str_mv El papel de las proteínas DELLA en el procesamiento alternativo de mensajeros
The role of ‘DELLA’ proteins in alternative splicing
title El papel de las proteínas DELLA en el procesamiento alternativo de mensajeros
spellingShingle El papel de las proteínas DELLA en el procesamiento alternativo de mensajeros
Calleja Cabrera, Julián
DELLA
Sm
LSM
Prefoldin
Arabidopsis
Alternative splicing
Gibberellins
Prefoldina
Splicing alternativo
BIOQUIMICA Y BIOLOGIA MOLECULAR
Máster Universitario en Biotecnología Molecular y Celular de Plantas-Màster Universitari en Biotecnologia Molecular i Cel·lular de Plantes
title_short El papel de las proteínas DELLA en el procesamiento alternativo de mensajeros
title_full El papel de las proteínas DELLA en el procesamiento alternativo de mensajeros
title_fullStr El papel de las proteínas DELLA en el procesamiento alternativo de mensajeros
title_full_unstemmed El papel de las proteínas DELLA en el procesamiento alternativo de mensajeros
title_sort El papel de las proteínas DELLA en el procesamiento alternativo de mensajeros
dc.creator.none.fl_str_mv Calleja Cabrera, Julián
author Calleja Cabrera, Julián
author_facet Calleja Cabrera, Julián
author_role author
dc.contributor.none.fl_str_mv Alabadí Diego, David
Esteve Bruna, David
Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv DELLA
Sm
LSM
Prefoldin
Arabidopsis
Alternative splicing
Gibberellins
Prefoldina
Splicing alternativo
BIOQUIMICA Y BIOLOGIA MOLECULAR
Máster Universitario en Biotecnología Molecular y Celular de Plantas-Màster Universitari en Biotecnologia Molecular i Cel·lular de Plantes
topic DELLA
Sm
LSM
Prefoldin
Arabidopsis
Alternative splicing
Gibberellins
Prefoldina
Splicing alternativo
BIOQUIMICA Y BIOLOGIA MOLECULAR
Máster Universitario en Biotecnología Molecular y Celular de Plantas-Màster Universitari en Biotecnologia Molecular i Cel·lular de Plantes
description DELLA proteins, the master repressors of the gibberellin (GA) signaling pathway, are thought to regulate gene expression by mechanisms other than their interaction with bona fide transcription factors. In particular, current work in our lab shows that DELLA and their atypical interactors prefoldins are able to interact with and regulate a number of proteins that are part of the basal transcriptional machinery, regulating the RNA polymerase II activity. Among the novel interactors being studied are proteins involved in mRNA splicing. In this TFM, we have investigated the participation of DELLA and prefoldin in alternative splicing by analyzing protein-protein interaction between these proteins and Sm/LSm, which form heteroheptameric complexes and are an essential part of the spliceosome. We have also advanced in obtaining resources to test genetic interactions between della and sm/lsm mutants. In addition, some alternative splicing events affected by DELLA activity identified in a transcriptomic analysis by RNA-seq, have been validated by semiquantitative RT-PCR. Finally, it has been shown that GA signaling alters the Sm and LSm protein levels.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-02-13
2018
2018-01-24
dc.type.none.fl_str_mv master thesis
http://purl.org/coar/resource_type/c_bdcc
dc.type.openaire.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/97802
url https://riunet.upv.es/handle/10251/97802
dc.language.none.fl_str_mv Español
spa
language_invalid_str_mv Español
language spa
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universitat Politècnica de València
publisher.none.fl_str_mv Universitat Politècnica de València
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.198674

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