Caracterización de la interacción entre las proteínas DELLA y la subunidad MED31 del complejo MEDIATOR en Arabidopsis
[EN] In our laboratory we are investigating about the molecular mechanisms by which DELLA proteins regulate gene expression in plants. DELLA are the negative regulators of the signaling pathway for gibberellins and their levels are negatively regulated by this hormone. The results obtained in the la...
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| Tipo de recurso: | tesis de maestría |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | español |
| OAI Identifier: | oai:riunet.upv.es:10251/97642 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/97642 |
| Access Level: | acceso abierto |
| Palabra clave: | Gibberellins Transcription DELLA Mediator RNA Pol II Arabidopsis Transcripción Giberelinas BIOQUIMICA Y BIOLOGIA MOLECULAR Máster Universitario en Biotecnología Molecular y Celular de Plantas-Màster Universitari en Biotecnologia Molecular i Cel·lular de Plantes |
| Sumario: | [EN] In our laboratory we are investigating about the molecular mechanisms by which DELLA proteins regulate gene expression in plants. DELLA are the negative regulators of the signaling pathway for gibberellins and their levels are negatively regulated by this hormone. The results obtained in the laboratory, together with those of others, have shown that an important part of this regulation occurs through the physical interaction of DELLA proteins with transcription factors, in some cases inactivating them, while in others the interaction promotes their activity. The latest results of the laboratory suggest that DELLA can act through other mechanisms to regulate gene expression, in particular, physical interactions have been found with several proteins that directly regulate RNA Pol II activity. One of these proteins is MED31. MED31 is part of the Mediator complex, a multiprotein complex conserved from yeast to humans which assists RNA Pol II practically all the stages of gene transcription, so it’s considered as a transcriptional activator. All in all, these preliminary results suggest that DELLA could regulate the transcription of certain genes modulating the activity of the Mediator complex and therefore of the RNA Pol II through the interaction with subunit MED31. Previous work in the laboratory has shown that a hypomorphic mutation med31 has very important consequences in plant development, pointing out to a relevant function. In this TFM context, we will try to (i) obtain new med31 alleles of Arabidopsis by amiRNA; (ii) characterize the interaction between DELLA and MED31 proteins in Nicotiana benthamiana and Arabidopsis plants and (iii) evaluate the relevance of the interaction through transcriptomic and physiological analysis. |
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