Novel Insights into the Nature of Intraspecific Genome Size Diversity in Cannabis sativa L.

Cannabis sativa has been used for millennia in traditional medicine for ritual purposes and for the production of food and fibres, thus, providing important and versatile services to humans. The species, which currently has a worldwide distribution, strikes out for displaying a huge morphological an...

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Autores: Balant, Manica, Rodríguez González, Roi, Garcia, Sònia, Garnatje, Teresa, Pellicer, Jaume, Vallès, Joan, Vitales, Daniel, Hidalgo, Oriane
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/281598
Acceso en línea:http://hdl.handle.net/10261/281598
https://doi.org/10.3390/plants11202736
Access Level:acceso abierto
Palabra clave:Cannabaceae
Cannabis sativa
Genome size
Intraspecific genome size variation
Population variability
Sex chromosomes
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spelling Novel Insights into the Nature of Intraspecific Genome Size Diversity in Cannabis sativa L.Balant, ManicaRodríguez González, RoiGarcia, SòniaGarnatje, TeresaPellicer, JaumeVallès, JoanVitales, DanielHidalgo, OrianeCannabaceaeCannabis sativaGenome sizeIntraspecific genome size variationPopulation variabilitySex chromosomesCannabis sativa has been used for millennia in traditional medicine for ritual purposes and for the production of food and fibres, thus, providing important and versatile services to humans. The species, which currently has a worldwide distribution, strikes out for displaying a huge morphological and chemical diversity. Differences in Cannabis genome size have also been found, suggesting it could be a useful character to differentiate between accessions. We used flow cytometry to investigate the extent of genome size diversity across 483 individuals belonging to 84 accessions, with a wide range of wild/feral, landrace, and cultivated accessions. We also carried out sex determination using the MADC2 marker and investigated the potential of flow cytometry as a method for early sex determination. All individuals were diploid, with genome sizes ranging from 1.810 up to 2.152 pg/2C (1.189-fold variation), apart from a triploid, with 2.884 pg/2C. Our results suggest that the geographical expansion of Cannabis and its domestication had little impact on its overall genome size. We found significant differences between the genome size of male and female individuals. Unfortunately, differences were, however, too small to be discriminated using flow cytometry through the direct processing of combined male and female individuals.This research was funded by Ministerio de Ciencia, Innovación y Universidades, grant numbers CGL2017-84297-R, PRE2018-083226, Generalitat de Catalunya, grant number 2017SGR1116, S.G. and J.P. benefited from a Ramón y Cajal grant funded by MCIN/AEI/ 10.13039/501100011033 and by “ESF Investing in your future”, grant numbers RYC-2014-16608 and RYC-2017-2274.1. Introduction 2. Results and Discussion 2.1. Genome Size in Cannabis: Evidence of Intraspecific Variation 2.2. Potential Factors Influencing Genome Size Variation in Cannabis 2.3. Events of Polyploidy in Cannabis Are Extremely Rare 2.4. Differences in Genome Size Values of Male and Female Cannabis Individuals 2.5. Sex Determination in Cannabis Using Flow Cytometry 3. Materials and Methods 3.1. Plant Sampling and Cultivation 3.2. Flow Cytometry Measurements 3.3. Chromosome Counts 3.4. Sex Determination Using Male-Associated Marker and Flow Cytometry 3.5. Statistical Analyses 4. Conclusions Supplementary Materials Author Contributions Funding Institutional Review Board Statement Informed Consent Statement Data Availability Statement Acknowledgments Conflicts of Interest ReferencesMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Generalitat de CatalunyaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/281598https://doi.org/10.3390/plants11202736reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2017-84297-Rinfo:eu-repo/grantAgreement/MINECO//RYC-2014-16608info:eu-repo/grantAgreement/MICIU/ICTI2017-2020/RYC-2017-2274https://doi.org/10.3390/plants11202736Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2815982026-05-22T06:33:51Z
dc.title.none.fl_str_mv Novel Insights into the Nature of Intraspecific Genome Size Diversity in Cannabis sativa L.
title Novel Insights into the Nature of Intraspecific Genome Size Diversity in Cannabis sativa L.
spellingShingle Novel Insights into the Nature of Intraspecific Genome Size Diversity in Cannabis sativa L.
Balant, Manica
Cannabaceae
Cannabis sativa
Genome size
Intraspecific genome size variation
Population variability
Sex chromosomes
title_short Novel Insights into the Nature of Intraspecific Genome Size Diversity in Cannabis sativa L.
title_full Novel Insights into the Nature of Intraspecific Genome Size Diversity in Cannabis sativa L.
title_fullStr Novel Insights into the Nature of Intraspecific Genome Size Diversity in Cannabis sativa L.
title_full_unstemmed Novel Insights into the Nature of Intraspecific Genome Size Diversity in Cannabis sativa L.
title_sort Novel Insights into the Nature of Intraspecific Genome Size Diversity in Cannabis sativa L.
dc.creator.none.fl_str_mv Balant, Manica
Rodríguez González, Roi
Garcia, Sònia
Garnatje, Teresa
Pellicer, Jaume
Vallès, Joan
Vitales, Daniel
Hidalgo, Oriane
author Balant, Manica
author_facet Balant, Manica
Rodríguez González, Roi
Garcia, Sònia
Garnatje, Teresa
Pellicer, Jaume
Vallès, Joan
Vitales, Daniel
Hidalgo, Oriane
author_role author
author2 Rodríguez González, Roi
Garcia, Sònia
Garnatje, Teresa
Pellicer, Jaume
Vallès, Joan
Vitales, Daniel
Hidalgo, Oriane
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Generalitat de Catalunya
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cannabaceae
Cannabis sativa
Genome size
Intraspecific genome size variation
Population variability
Sex chromosomes
topic Cannabaceae
Cannabis sativa
Genome size
Intraspecific genome size variation
Population variability
Sex chromosomes
description Cannabis sativa has been used for millennia in traditional medicine for ritual purposes and for the production of food and fibres, thus, providing important and versatile services to humans. The species, which currently has a worldwide distribution, strikes out for displaying a huge morphological and chemical diversity. Differences in Cannabis genome size have also been found, suggesting it could be a useful character to differentiate between accessions. We used flow cytometry to investigate the extent of genome size diversity across 483 individuals belonging to 84 accessions, with a wide range of wild/feral, landrace, and cultivated accessions. We also carried out sex determination using the MADC2 marker and investigated the potential of flow cytometry as a method for early sex determination. All individuals were diploid, with genome sizes ranging from 1.810 up to 2.152 pg/2C (1.189-fold variation), apart from a triploid, with 2.884 pg/2C. Our results suggest that the geographical expansion of Cannabis and its domestication had little impact on its overall genome size. We found significant differences between the genome size of male and female individuals. Unfortunately, differences were, however, too small to be discriminated using flow cytometry through the direct processing of combined male and female individuals.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/281598
https://doi.org/10.3390/plants11202736
url http://hdl.handle.net/10261/281598
https://doi.org/10.3390/plants11202736
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2017-84297-R
info:eu-repo/grantAgreement/MINECO//RYC-2014-16608
info:eu-repo/grantAgreement/MICIU/ICTI2017-2020/RYC-2017-2274
https://doi.org/10.3390/plants11202736
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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