Diversity, dynamics and activity of Epsilonproteobacteria in a stratified karstic lake. Implications in carbon and sulfur cycles
This doctoral thesis studies the abundance, diversity, seasonal dynamics and activity of Epsilonproteobacteria in a meromictic basin of Lake Banyoles during three year cycles. We applied a complementary array of molecular techniques to resolve the identity and ecological role of Epsilonproteobacteri...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/403813 |
| Acceso en línea: | http://hdl.handle.net/10803/403813 |
| Access Level: | acceso abierto |
| Palabra clave: | Epsilonproteobacteria Arcobacter Ecologia microbiana Microbial ecology Llacs estratificats Stratified lakes Lagos estratificados Fixació fosca de carboni inorgànic Dark inorganic carbon fixation Fijación oscura de carbono inorgánico Estany de Banyoles Banyoles Lake Lago de Banyoles 574 579 |
| Sumario: | This doctoral thesis studies the abundance, diversity, seasonal dynamics and activity of Epsilonproteobacteria in a meromictic basin of Lake Banyoles during three year cycles. We applied a complementary array of molecular techniques to resolve the identity and ecological role of Epsilonproteobacteria in the system, with special focus on the contribution of these microorganisms on linking C and S cycles. Our results pointed to a clear seasonality of Epsilonproteobacteria, with maximal abundances at the redoxcline and upper monimolimnion in winter. Furthermore, results revealed the predominance of sequences affiliated to genus Arcobacter, and clearly indicated that Epsilonproteobacteria actively assimilated CO2 in the dark thus being responsible of the high rates of dark carbon fixation measured at the redoxcline in winter. Additional analyses provided additional evidence that the dominant member of the epsilonproteobacterial community is a chemolithotrophic, sulfide-oxidizing member of the genus Arcobacter, distantly related to its marine counterpart Candidatus A.sulfidicus. |
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