Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN).
Enteroviruses (EVs) are a common cause of a wide spectrum of infectious diseases, ranging from mild respiratory illnesses to severe neurological conditions, particularly affecting children. Current molecular methods, such as 5'UTR-based PCR for detection and (partial) VP1 gene sequencing for ty...
| Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2026 |
| País: | España |
| Institución: | Fundació Sant Joan de Déu |
| Repositorio: | r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu |
| OAI Identifier: | oai:dnet:r-fsjd______::e6dc2f3c7bc769c4202b05a5194d4f9a |
| Acceso en línea: | https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=30174 |
| Access Level: | acceso abierto |
| Palabra clave: | Bioinformatics Dry-lab Enteroviruses Next Generation Sequencing Targeted PCR-based Viral metagenomics Wet-lab |
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Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN).Benschop KSMZwagemaker FAndersson-Li LAndrés CAntón ABerengua CBerginc NBessaud MBisseux MBujaki ECanning BChristiansen CBCouderé KBroberg EKCassidy HCastilletti CCelma CCinek ODeézsi-Magyar NEis-Hübinger AMFlipse JJirincová HGatej RGeorgieva IGiardina FGonzález-Sánchez AHack BHelfferich JHutchings SHietanen EHönemann MVirant MJKalogera EJohannesen CKKenicer JKleines MLagarejos ELandaas ETKandulu CCXavier López Labrador FLunar MMMaier MMajumdar MMartin JMcClure CPMuñoz-Almagro COšep AØverbø JPalminha PPapa APariani EPellegrinelli LPietsch CPiralla APoljak MPomari EPrats-Méndez IRector AReuter GRiess MRuta SSchibler MNilsen HSSimmonds PSourvinos GSzomor KSusi PTabain IVallely Pvon Eije KJWeil MWieczorek MWollants EWolthers KCZuckerman NSFischer TKHarvala HBioinformaticsDry-labEnterovirusesNext Generation SequencingTargeted PCR-basedViral metagenomicsWet-labEnteroviruses (EVs) are a common cause of a wide spectrum of infectious diseases, ranging from mild respiratory illnesses to severe neurological conditions, particularly affecting children. Current molecular methods, such as 5'UTR-based PCR for detection and (partial) VP1 gene sequencing for typing, are widely utilized. However, Next-Generation Sequencing (NGS), and bioinformatics offer a comprehensive alternative, enabling full-genome analyses for improved virus characterization, genomic epidemiological surveillance, and outbreak investigation. Despite its advantages, implementation of NGS poses challenges, particularly in standardizing and optimizing laboratory workflows (wet-lab) and bioinformatics analyses (dry-lab), methods that are not often readily accessible in many laboratories. Here, we discuss the potential of NGS as a tool for EV detection/characterization in clinical virology, public health, and research settings. We provide practical options for actions for implementing NGS to advance the understanding and management of enterovirus infections. These recommendations are based on expert discussions during the recent European non-polio enterovirus network (ENPEN) workshop held in Corfu, Greece, on 23-24 May 2024, aiming to guide harmonization of NGS practices across clinical, public health, and research settings.ELSEVIER2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=30174JOURNAL OF CLINICAL VIROLOGYISSN: 13866532ISSNe: 18735967reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déuinstname:Fundació Sant Joan de DéuInglésinfo:eu-repo/semantics/openAccessoai:dnet:r-fsjd______::e6dc2f3c7bc769c4202b05a5194d4f9a2026-05-27T12:37:41Z |
| dc.title.none.fl_str_mv |
Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN). |
| title |
Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN). |
| spellingShingle |
Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN). Benschop KSM Bioinformatics Dry-lab Enteroviruses Next Generation Sequencing Targeted PCR-based Viral metagenomics Wet-lab |
| title_short |
Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN). |
| title_full |
Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN). |
| title_fullStr |
Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN). |
| title_full_unstemmed |
Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN). |
| title_sort |
Next generation sequencing approaches for the detection and characterization of enteroviruses in clinical, public health, and research settings: Expert view of the European non-polio enterovirus network (ENPEN). |
| dc.creator.none.fl_str_mv |
Benschop KSM Zwagemaker F Andersson-Li L Andrés C Antón A Berengua C Berginc N Bessaud M Bisseux M Bujaki E Canning B Christiansen CB Couderé K Broberg EK Cassidy H Castilletti C Celma C Cinek O Deézsi-Magyar N Eis-Hübinger AM Flipse J Jirincová H Gatej R Georgieva I Giardina F González-Sánchez A Hack B Helfferich J Hutchings S Hietanen E Hönemann M Virant MJ Kalogera E Johannesen CK Kenicer J Kleines M Lagarejos E Landaas ET Kandulu CC Xavier López Labrador F Lunar MM Maier M Majumdar M Martin J McClure CP Muñoz-Almagro C Ošep A Øverbø J Palminha P Papa A Pariani E Pellegrinelli L Pietsch C Piralla A Poljak M Pomari E Prats-Méndez I Rector A Reuter G Riess M Ruta S Schibler M Nilsen HS Simmonds P Sourvinos G Szomor K Susi P Tabain I Vallely P von Eije KJ Weil M Wieczorek M Wollants E Wolthers KC Zuckerman NS Fischer TK Harvala H |
| author |
Benschop KSM |
| author_facet |
Benschop KSM Zwagemaker F Andersson-Li L Andrés C Antón A Berengua C Berginc N Bessaud M Bisseux M Bujaki E Canning B Christiansen CB Couderé K Broberg EK Cassidy H Castilletti C Celma C Cinek O Deézsi-Magyar N Eis-Hübinger AM Flipse J Jirincová H Gatej R Georgieva I Giardina F González-Sánchez A Hack B Helfferich J Hutchings S Hietanen E Hönemann M Virant MJ Kalogera E Johannesen CK Kenicer J Kleines M Lagarejos E Landaas ET Kandulu CC Xavier López Labrador F Lunar MM Maier M Majumdar M Martin J McClure CP Muñoz-Almagro C Ošep A Øverbø J Palminha P Papa A Pariani E Pellegrinelli L Pietsch C Piralla A Poljak M Pomari E Prats-Méndez I Rector A Reuter G Riess M Ruta S Schibler M Nilsen HS Simmonds P Sourvinos G Szomor K Susi P Tabain I Vallely P von Eije KJ Weil M Wieczorek M Wollants E Wolthers KC Zuckerman NS Fischer TK Harvala H |
| author_role |
author |
| author2 |
Zwagemaker F Andersson-Li L Andrés C Antón A Berengua C Berginc N Bessaud M Bisseux M Bujaki E Canning B Christiansen CB Couderé K Broberg EK Cassidy H Castilletti C Celma C Cinek O Deézsi-Magyar N Eis-Hübinger AM Flipse J Jirincová H Gatej R Georgieva I Giardina F González-Sánchez A Hack B Helfferich J Hutchings S Hietanen E Hönemann M Virant MJ Kalogera E Johannesen CK Kenicer J Kleines M Lagarejos E Landaas ET Kandulu CC Xavier López Labrador F Lunar MM Maier M Majumdar M Martin J McClure CP Muñoz-Almagro C Ošep A Øverbø J Palminha P Papa A Pariani E Pellegrinelli L Pietsch C Piralla A Poljak M Pomari E Prats-Méndez I Rector A Reuter G Riess M Ruta S Schibler M Nilsen HS Simmonds P Sourvinos G Szomor K Susi P Tabain I Vallely P von Eije KJ Weil M Wieczorek M Wollants E Wolthers KC Zuckerman NS Fischer TK Harvala H |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Bioinformatics Dry-lab Enteroviruses Next Generation Sequencing Targeted PCR-based Viral metagenomics Wet-lab |
| topic |
Bioinformatics Dry-lab Enteroviruses Next Generation Sequencing Targeted PCR-based Viral metagenomics Wet-lab |
| description |
Enteroviruses (EVs) are a common cause of a wide spectrum of infectious diseases, ranging from mild respiratory illnesses to severe neurological conditions, particularly affecting children. Current molecular methods, such as 5'UTR-based PCR for detection and (partial) VP1 gene sequencing for typing, are widely utilized. However, Next-Generation Sequencing (NGS), and bioinformatics offer a comprehensive alternative, enabling full-genome analyses for improved virus characterization, genomic epidemiological surveillance, and outbreak investigation. Despite its advantages, implementation of NGS poses challenges, particularly in standardizing and optimizing laboratory workflows (wet-lab) and bioinformatics analyses (dry-lab), methods that are not often readily accessible in many laboratories. Here, we discuss the potential of NGS as a tool for EV detection/characterization in clinical virology, public health, and research settings. We provide practical options for actions for implementing NGS to advance the understanding and management of enterovirus infections. These recommendations are based on expert discussions during the recent European non-polio enterovirus network (ENPEN) workshop held in Corfu, Greece, on 23-24 May 2024, aiming to guide harmonization of NGS practices across clinical, public health, and research settings. |
| publishDate |
2026 |
| dc.date.none.fl_str_mv |
2026 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=30174 |
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https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=30174 |
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Inglés |
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Inglés |
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info:eu-repo/semantics/openAccess |
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openAccess |
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ELSEVIER |
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ELSEVIER |
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JOURNAL OF CLINICAL VIROLOGY ISSN: 13866532 ISSNe: 18735967 reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu instname:Fundació Sant Joan de Déu |
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Fundació Sant Joan de Déu |
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r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu |
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r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu |
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