Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure.

Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the deve...

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Bibliographic Details
Authors: Lopez-Labrador, F Xavier, Brown, Julianne R, Fischer, Nicole, Harvala, Heli, Van Boheemen, Sander, Cinek, Ondrej, Sayiner, Arzu, Madsen, Tina Vasehus, Auvinen, Eeva, Kufner, Verena, Huber, Michael, Rodriguez, Christophe, Jonges, Marcel, Honemann, Mario, Susi, Petri, Sousa, Hugo, Klapper, Paul E, Perez-Cataluna, Alba, Hernandez, Marta, Molenkamp, Richard, der Hoek, Lia van, Schuurman, Rob, Couto, Natacha, Leuzinger, Karoline, Simmonds, Peter, Beer, Martin, Hoper, Dirk, Kamminga, Sergio, Feltkamp, Mariet C W, Rodriguez-Diaz, Jesus, Keyaerts, Els, Nielsen, Xiaohui Chen, Puchhammer-Stockl, Elisabeth, Kroes, Aloys C M, Buesa, Javier, Breuer, Judy, Claas, Eric C J, de Vries, Jutte J C, ESCV Network on Next-Generation Sequencing
Format: article
Status:Published version
Publication Date:2021
Country:España
Institution:INCLIVA
Repository:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
OAI Identifier:oai:incliva.fundanetsuite.com:p15207
Online Access:https://incliva.portalinvestigacion.com/publicaciones/15207
Access Level:Open access
Keyword:High-throughput sequencing
Next-generation sequencing
Recommendations
Viral metagenomics
Wet lab
Description
Summary:Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.

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