Dendrimersome Synthetic Cells Harbor Cell Division Machinery of Bacteria

The integration of active cell machinery with synthetic building blocks is the bridge toward developing synthetic cells with biological functions and beyond. Self-replication is one of the most important tasks of living systems, and various complex machineries exist to execute it. In Escherichia col...

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Autores: Wagner, Anna M., Eto, Hiromune, Joseph, Anton, Kohyama, Shunshi, Haraszti, Tamás, Zamora, Ricardo A., Vorobii, Mariia, Giannotti, Marina Inés, Schwille, Petra, Rodriguez Emmenegger, César
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/186960
Acceso en línea:https://hdl.handle.net/2445/186960
Access Level:acceso abierto
Palabra clave:Biomimètica
Fisiologia
Biomimetics
Physiology
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spelling Dendrimersome Synthetic Cells Harbor Cell Division Machinery of BacteriaWagner, Anna M.Eto, HiromuneJoseph, AntonKohyama, ShunshiHaraszti, TamásZamora, Ricardo A.Vorobii, MariiaGiannotti, Marina InésSchwille, PetraRodriguez Emmenegger, CésarBiomimèticaFisiologiaBiomimeticsPhysiologyThe integration of active cell machinery with synthetic building blocks is the bridge toward developing synthetic cells with biological functions and beyond. Self-replication is one of the most important tasks of living systems, and various complex machineries exist to execute it. In Escherichia coli, a contractile division ring is positioned to mid-cell by concentration oscillations of self-organizing proteins (MinCDE), where it severs membrane and cell wall. So far, the reconstitution of any cell division machinery has exclusively been tied to liposomes. Here, the reconstitution of a rudimentary bacterial divisome in fully synthetic bicomponent dendrimersomes is shown. By tuning the membrane composition, the interaction of biological machinery with synthetic membranes can be tailored to reproduce its dynamic behavior. This constitutes an important breakthrough in the assembly of synthetic cells with biological elements, as tuning of membrane-divisome interactions is the key to engineering emergent biological behavior from the bottom-up.Wiley2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/186960Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1002/adma.202202364Advanced Materials, 2022https://doi.org/10.1002/adma.202202364cc by-nc (c) Wagner, Anna M. et al, 2022http://creativecommons.org/licenses/by-nc/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1869602026-05-27T06:46:51Z
dc.title.none.fl_str_mv Dendrimersome Synthetic Cells Harbor Cell Division Machinery of Bacteria
title Dendrimersome Synthetic Cells Harbor Cell Division Machinery of Bacteria
spellingShingle Dendrimersome Synthetic Cells Harbor Cell Division Machinery of Bacteria
Wagner, Anna M.
Biomimètica
Fisiologia
Biomimetics
Physiology
title_short Dendrimersome Synthetic Cells Harbor Cell Division Machinery of Bacteria
title_full Dendrimersome Synthetic Cells Harbor Cell Division Machinery of Bacteria
title_fullStr Dendrimersome Synthetic Cells Harbor Cell Division Machinery of Bacteria
title_full_unstemmed Dendrimersome Synthetic Cells Harbor Cell Division Machinery of Bacteria
title_sort Dendrimersome Synthetic Cells Harbor Cell Division Machinery of Bacteria
dc.creator.none.fl_str_mv Wagner, Anna M.
Eto, Hiromune
Joseph, Anton
Kohyama, Shunshi
Haraszti, Tamás
Zamora, Ricardo A.
Vorobii, Mariia
Giannotti, Marina Inés
Schwille, Petra
Rodriguez Emmenegger, César
author Wagner, Anna M.
author_facet Wagner, Anna M.
Eto, Hiromune
Joseph, Anton
Kohyama, Shunshi
Haraszti, Tamás
Zamora, Ricardo A.
Vorobii, Mariia
Giannotti, Marina Inés
Schwille, Petra
Rodriguez Emmenegger, César
author_role author
author2 Eto, Hiromune
Joseph, Anton
Kohyama, Shunshi
Haraszti, Tamás
Zamora, Ricardo A.
Vorobii, Mariia
Giannotti, Marina Inés
Schwille, Petra
Rodriguez Emmenegger, César
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biomimètica
Fisiologia
Biomimetics
Physiology
topic Biomimètica
Fisiologia
Biomimetics
Physiology
description The integration of active cell machinery with synthetic building blocks is the bridge toward developing synthetic cells with biological functions and beyond. Self-replication is one of the most important tasks of living systems, and various complex machineries exist to execute it. In Escherichia coli, a contractile division ring is positioned to mid-cell by concentration oscillations of self-organizing proteins (MinCDE), where it severs membrane and cell wall. So far, the reconstitution of any cell division machinery has exclusively been tied to liposomes. Here, the reconstitution of a rudimentary bacterial divisome in fully synthetic bicomponent dendrimersomes is shown. By tuning the membrane composition, the interaction of biological machinery with synthetic membranes can be tailored to reproduce its dynamic behavior. This constitutes an important breakthrough in the assembly of synthetic cells with biological elements, as tuning of membrane-divisome interactions is the key to engineering emergent biological behavior from the bottom-up.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/186960
url https://hdl.handle.net/2445/186960
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1002/adma.202202364
Advanced Materials, 2022
https://doi.org/10.1002/adma.202202364
dc.rights.none.fl_str_mv cc by-nc (c) Wagner, Anna M. et al, 2022
http://creativecommons.org/licenses/by-nc/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by-nc (c) Wagner, Anna M. et al, 2022
http://creativecommons.org/licenses/by-nc/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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