Dendrimersome Synthetic Cells Harbor Cell Division Machinery of Bacteria

The integration of active cell machinery with synthetic building blocks is the bridge toward developing synthetic cells with biological functions and beyond. Self-replication is one of the most important tasks of living systems, and various complex machineries exist to execute it. In Escherichia col...

Descripción completa

Detalles Bibliográficos
Autores: Wagner, Anna M., Eto, Hiromune, Joseph, Anton, Kohyama, Shunshi, Haraszti, Tamás, Zamora, Ricardo A., Vorobii, Mariia, Giannotti, Marina Inés, Schwille, Petra, Rodriguez Emmenegger, César
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/186960
Acceso en línea:https://hdl.handle.net/2445/186960
Access Level:acceso abierto
Palabra clave:Biomimètica
Fisiologia
Biomimetics
Physiology
Descripción
Sumario:The integration of active cell machinery with synthetic building blocks is the bridge toward developing synthetic cells with biological functions and beyond. Self-replication is one of the most important tasks of living systems, and various complex machineries exist to execute it. In Escherichia coli, a contractile division ring is positioned to mid-cell by concentration oscillations of self-organizing proteins (MinCDE), where it severs membrane and cell wall. So far, the reconstitution of any cell division machinery has exclusively been tied to liposomes. Here, the reconstitution of a rudimentary bacterial divisome in fully synthetic bicomponent dendrimersomes is shown. By tuning the membrane composition, the interaction of biological machinery with synthetic membranes can be tailored to reproduce its dynamic behavior. This constitutes an important breakthrough in the assembly of synthetic cells with biological elements, as tuning of membrane-divisome interactions is the key to engineering emergent biological behavior from the bottom-up.