Immunohistochemical Expression of PTEN in Canine Gliomas

Phosphatase and tensin homolog (PTEN) is a critical tumor suppressor gene with a vital role in regulating cell proliferation, migration, and survival. The loss of PTEN function, either by genetic alterations or decreased protein expression, is frequent in human gliomas and has been correlated with t...

Descripción completa

Detalles Bibliográficos
Autores: Molín Molina, Jéssica, José-López, Roberto, Ramírez, Gustavo A., Pumarola Batlle, Martí
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/466706
Acceso en línea:https://doi.org/10.3390/ani14142115
https://hdl.handle.net/10459.1/466706
Access Level:acceso abierto
Palabra clave:Astrocytoma
Dog
High grade
Immunohistochemistry
Oligodendroglioma
PTEN
PI3K/Akt/mTor
id ES_3f3f356c69f6ecc2c57bf2ca7a5aa4b9
oai_identifier_str oai:repositori.udl.cat:10459.1/466706
network_acronym_str ES
network_name_str España
repository_id_str
spelling Immunohistochemical Expression of PTEN in Canine GliomasMolín Molina, JéssicaJosé-López, RobertoRamírez, Gustavo A.Pumarola Batlle, MartíAstrocytomaDogHigh gradeImmunohistochemistryOligodendrogliomaPTENPI3K/Akt/mTorPhosphatase and tensin homolog (PTEN) is a critical tumor suppressor gene with a vital role in regulating cell proliferation, migration, and survival. The loss of PTEN function, either by genetic alterations or decreased protein expression, is frequent in human gliomas and has been correlated with tumor progression, grade, therapeutic resistance, and decreased overall survival in patients with glioma. While different genetic mutations in PTEN gene have been occasionally reported in canine gliomas, no alterations in protein expression have been reported. This study investigates the immunohistochemical expression of PTEN in canine gliomas to evaluate possible alterations, as those reported in human gliomas. Immunohistochemical PTEN expression and pattern distribution were analyzed in 37 spontaneous canine gliomas. Among gliomas, 52.6% cases showed high PTEN expression and 48.6% displayed reduced (13.5%) or highly reduced (35.1%) immunopositivity. Most oligodendrogliomas showed high expression (73.7%), while the majority of astrocytomas (69.2%) showed a reduced or highly reduced expression. A reduced PTEN expression was mostly associated with a heterogeneous loss of PTEN immunopositivity. These observations are in line with those reported in human gliomas and provide a rationale for future studies regarding abnormalities in PTEN expression and PI3K/Akt/mTor pathway in canine gliomas, to evaluate its prognostic and therapeutic implications.This research received no external funding. The authors would like to acknowledge Ester Blasco, Tamara Navarro (Unitat de Patologia Murina i Comparada, Universtitat Autònoma de Barcelona), Maria Santacana, and Maria Carrelé (Institut de Recerca Biomèdica—IRB, Lleida) for the technical assistance, and Sandra Solé for her contribution to literature review and clinical data collection. We are also grateful to all veterinary neurologists for providing cases. J.M. is a Serra Húnter Fellow.MDPI2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.3390/ani14142115https://hdl.handle.net/10459.1/466706reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a: https://doi.org/10.3390/ani14142115Animals, 2024, vol. 14, num. 14cc-by,(c) Molín et al., 2024info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4667062026-06-24T12:42:17Z
dc.title.none.fl_str_mv Immunohistochemical Expression of PTEN in Canine Gliomas
title Immunohistochemical Expression of PTEN in Canine Gliomas
spellingShingle Immunohistochemical Expression of PTEN in Canine Gliomas
Molín Molina, Jéssica
Astrocytoma
Dog
High grade
Immunohistochemistry
Oligodendroglioma
PTEN
PI3K/Akt/mTor
title_short Immunohistochemical Expression of PTEN in Canine Gliomas
title_full Immunohistochemical Expression of PTEN in Canine Gliomas
title_fullStr Immunohistochemical Expression of PTEN in Canine Gliomas
title_full_unstemmed Immunohistochemical Expression of PTEN in Canine Gliomas
title_sort Immunohistochemical Expression of PTEN in Canine Gliomas
dc.creator.none.fl_str_mv Molín Molina, Jéssica
José-López, Roberto
Ramírez, Gustavo A.
Pumarola Batlle, Martí
author Molín Molina, Jéssica
author_facet Molín Molina, Jéssica
José-López, Roberto
Ramírez, Gustavo A.
Pumarola Batlle, Martí
author_role author
author2 José-López, Roberto
Ramírez, Gustavo A.
Pumarola Batlle, Martí
author2_role author
author
author
dc.subject.none.fl_str_mv Astrocytoma
Dog
High grade
Immunohistochemistry
Oligodendroglioma
PTEN
PI3K/Akt/mTor
topic Astrocytoma
Dog
High grade
Immunohistochemistry
Oligodendroglioma
PTEN
PI3K/Akt/mTor
description Phosphatase and tensin homolog (PTEN) is a critical tumor suppressor gene with a vital role in regulating cell proliferation, migration, and survival. The loss of PTEN function, either by genetic alterations or decreased protein expression, is frequent in human gliomas and has been correlated with tumor progression, grade, therapeutic resistance, and decreased overall survival in patients with glioma. While different genetic mutations in PTEN gene have been occasionally reported in canine gliomas, no alterations in protein expression have been reported. This study investigates the immunohistochemical expression of PTEN in canine gliomas to evaluate possible alterations, as those reported in human gliomas. Immunohistochemical PTEN expression and pattern distribution were analyzed in 37 spontaneous canine gliomas. Among gliomas, 52.6% cases showed high PTEN expression and 48.6% displayed reduced (13.5%) or highly reduced (35.1%) immunopositivity. Most oligodendrogliomas showed high expression (73.7%), while the majority of astrocytomas (69.2%) showed a reduced or highly reduced expression. A reduced PTEN expression was mostly associated with a heterogeneous loss of PTEN immunopositivity. These observations are in line with those reported in human gliomas and provide a rationale for future studies regarding abnormalities in PTEN expression and PI3K/Akt/mTor pathway in canine gliomas, to evaluate its prognostic and therapeutic implications.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/ani14142115
https://hdl.handle.net/10459.1/466706
url https://doi.org/10.3390/ani14142115
https://hdl.handle.net/10459.1/466706
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/ani14142115
Animals, 2024, vol. 14, num. 14
dc.rights.none.fl_str_mv cc-by,(c) Molín et al., 2024
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by,(c) Molín et al., 2024
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406620464709632
score 15,811543

Similares en LA Referencia