Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarum

Some Vibrio anguillarum strains produce a catechol-type siderophore named vanchrobactin, whose biosynthetic pathway has not been completely elucidated. In addition to the previously described genes vabA, vabC, vabB, vabE, vabF, vabS and vabH, in the present study we have identified the genes encodin...

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Detalhes bibliográficos
Autores: Balado Dacosta, Miguel, Rodríguez Osorio, Carlos, Lemos Ramos, Manuel Luis
Formato: artículo
Fecha de publicación:2008
País:España
Recursos:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/46359
Acesso em linha:https://hdl.handle.net/10347/46359
Access Level:acceso abierto
Palavra-chave:Vibrio anguillarum
Iron uptake
Siderophore biosynthesis
Vanchrobactin
Gene regulation
Fur
LysR-type Regulators
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spelling Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarumBalado Dacosta, MiguelRodríguez Osorio, CarlosLemos Ramos, Manuel LuisVibrio anguillarumIron uptakeSiderophore biosynthesisVanchrobactinGene regulationFurLysR-type RegulatorsSome Vibrio anguillarum strains produce a catechol-type siderophore named vanchrobactin, whose biosynthetic pathway has not been completely elucidated. In addition to the previously described genes vabA, vabC, vabB, vabE, vabF, vabS and vabH, in the present study we have identified the genes encoding a DAHP (3-deoxy-d-arabino-heptulosonate-7-phosphate) synthetase (vabG), a phosphopantheteinyl transferase (vabD), a LysR-family transcriptional regulator (vabR) and a putative siderophore receptor (fvtA). A deletion affecting vabG or vabD greatly reduced growth under iron-limiting conditions, whereas deletion of vabR did not have significant effects. Vanchrobactin production was abolished in the vabD mutant, whereas the vabG mutant retained a residual vanchrobactin production ability. Reverse transcriptase-mediated PCR indicated that this 11-gene cluster is organized into six iron-regulated transcriptional units. Transcriptional lacZ fusions demonstrated that the ferric uptake regulator (Fur) protein is the main iron-responsive regulator of these genes. Interestingly, the vabG gene was strongly iron-repressed, but Fur was not essential for this repression. In addition, the maximal expression from the vabG promoter was achieved only in the presence of an intact copy of vabR. Analysis of the β-galactosidase activities of a fvtA : : lacZ fusion in a vabB mutant and in the presence of added vanchrobactin suggested that a ferric-vanchrobactin-dependent activator plays a positive regulatory role in transcription of the fvtA–vabD operon. This possibility is reinforced by the presence of a predicted AraC box upstream of fvtA. We propose that vanchrobactin biosynthesis is subjected to a complex regulatory circuitry aimed at adjusting vanchrobactin production for the maintenance of iron homeostasis in V. anguillarum.Microbiology SocietyUniversidade de Santiago de Compostela. Instituto de Investigación do Medio Acuático para Unha Saúde Global (iARCUS)Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía20082008-05-0120082008-05-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/46359reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)InglésengMinisterio de Educación y Ciencia Otro AGL2006-00697 ESTUDIO DE LOS MECANISMOS DE ASIMILACION DE HIERRO BASADOS EN LA SINTESIS DE SIDEROFOROS COMO FACTORES DE VIRULENCIA EN LOS PATOGENOS DE PECES VIBRIO ANGUILLARUM O2 Y PHOTOBACTERIUM DAMSELAEopen accesshttp://purl.org/coar/access_right/c_abf2© Balado, M., Osorio, C. R., & Lemos, M. L. The definitive peer reviewed, edited version of this article is published in Microbiology, 154(5), 2008, 10.1099/mic.0.2008/016618-0http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/463592026-06-15T12:47:27Z
dc.title.none.fl_str_mv Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarum
title Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarum
spellingShingle Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarum
Balado Dacosta, Miguel
Vibrio anguillarum
Iron uptake
Siderophore biosynthesis
Vanchrobactin
Gene regulation
Fur
LysR-type Regulators
title_short Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarum
title_full Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarum
title_fullStr Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarum
title_full_unstemmed Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarum
title_sort Biosynthetic and regulatory elements involved in the production of the siderophore vanchrobactin in Vibrio anguillarum
dc.creator.none.fl_str_mv Balado Dacosta, Miguel
Rodríguez Osorio, Carlos
Lemos Ramos, Manuel Luis
author Balado Dacosta, Miguel
author_facet Balado Dacosta, Miguel
Rodríguez Osorio, Carlos
Lemos Ramos, Manuel Luis
author_role author
author2 Rodríguez Osorio, Carlos
Lemos Ramos, Manuel Luis
author2_role author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Instituto de Investigación do Medio Acuático para Unha Saúde Global (iARCUS)
Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía
dc.subject.none.fl_str_mv Vibrio anguillarum
Iron uptake
Siderophore biosynthesis
Vanchrobactin
Gene regulation
Fur
LysR-type Regulators
topic Vibrio anguillarum
Iron uptake
Siderophore biosynthesis
Vanchrobactin
Gene regulation
Fur
LysR-type Regulators
description Some Vibrio anguillarum strains produce a catechol-type siderophore named vanchrobactin, whose biosynthetic pathway has not been completely elucidated. In addition to the previously described genes vabA, vabC, vabB, vabE, vabF, vabS and vabH, in the present study we have identified the genes encoding a DAHP (3-deoxy-d-arabino-heptulosonate-7-phosphate) synthetase (vabG), a phosphopantheteinyl transferase (vabD), a LysR-family transcriptional regulator (vabR) and a putative siderophore receptor (fvtA). A deletion affecting vabG or vabD greatly reduced growth under iron-limiting conditions, whereas deletion of vabR did not have significant effects. Vanchrobactin production was abolished in the vabD mutant, whereas the vabG mutant retained a residual vanchrobactin production ability. Reverse transcriptase-mediated PCR indicated that this 11-gene cluster is organized into six iron-regulated transcriptional units. Transcriptional lacZ fusions demonstrated that the ferric uptake regulator (Fur) protein is the main iron-responsive regulator of these genes. Interestingly, the vabG gene was strongly iron-repressed, but Fur was not essential for this repression. In addition, the maximal expression from the vabG promoter was achieved only in the presence of an intact copy of vabR. Analysis of the β-galactosidase activities of a fvtA : : lacZ fusion in a vabB mutant and in the presence of added vanchrobactin suggested that a ferric-vanchrobactin-dependent activator plays a positive regulatory role in transcription of the fvtA–vabD operon. This possibility is reinforced by the presence of a predicted AraC box upstream of fvtA. We propose that vanchrobactin biosynthesis is subjected to a complex regulatory circuitry aimed at adjusting vanchrobactin production for the maintenance of iron homeostasis in V. anguillarum.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-05-01
2008
2008-05-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/46359
url https://hdl.handle.net/10347/46359
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Educación y Ciencia Otro AGL2006-00697 ESTUDIO DE LOS MECANISMOS DE ASIMILACION DE HIERRO BASADOS EN LA SINTESIS DE SIDEROFOROS COMO FACTORES DE VIRULENCIA EN LOS PATOGENOS DE PECES VIBRIO ANGUILLARUM O2 Y PHOTOBACTERIUM DAMSELAE
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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