Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper
The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to ot...
| Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2017 |
| País: | España |
| Recursos: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/97155 |
| Acesso em linha: | https://hdl.handle.net/20.500.14352/97155 |
| Access Level: | acceso abierto |
| Palavra-chave: | 576.3 Extracellular vesicles Exosomes Non-coding RNA microRNA mRNA RNA sorting RNA binding proteins Quantification Biología celular (Biología) Biología molecular (Biología) 2407 Biología Celular 2415 Biología Molecular 2410.03 Citología Humana |
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Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paperMateescu, BogdanKowal, EmmaVan Balkom, BasBartel, SabineBhattacharyya, SuvendraBuzás, EditBuck, AmyCandia, Paola deChow, FranklinDas, SaumyaDriedonks, TomFernández Messina, Lola MaríaHaderk, FranziskaHill, AndrewJones, JenniferVan Keuren‐Jensen, KendallLai, CharlesLässer, Ceciliadi Liegro, ItaliaLunavat, TaralLorenowicz, MagdalenaMaas, SybrenMäger, ImreMittelbrunn, MariaMomma, StefanMukherjee, KamalikaNawaz, MuhammadPegtel, MichielPfaffl, MichaelSchiffelers, RaymondTahara, HidetoshiThéry, ClotildeTosar, Juan PabloWauben, MarcaWitwer, KennethNolte‐'t Hoen, Esther576.3Extracellular vesiclesExosomesNon-coding RNAmicroRNAmRNARNA sortingRNA binding proteinsQuantificationBiología celular (Biología)Biología molecular (Biología)2407 Biología Celular2415 Biología Molecular2410.03 Citología HumanaThe release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells. Moreover, EV-RNA research faces various technical challenges, such as standardisation of EV isolation methods, optimisation of methodologies to isolate and characterise minute quantities of RNA found in EV, and development of approaches to demonstrate functional transfer of EV-RNA in vivo. These topics were discussed at the 2015 EV-RNA workshop of the International Society for Extracellular Vesicles. This position paper was written by the participants of the workshop not only to give an overview of the current state of knowledge in the field, but also to clarify that our incomplete knowledge – of the nature of EV(-RNA)s and of how to effectively and reliably study them – currently prohibits the implementation of gold standards in EV-RNA research. In addition, this paper creates awareness of possibilities and limitations of currently used strategies to investigate EV-RNA and calls for caution in interpretation of the obtained data.WileyUniversidad Complutense de Madrid20172017-01-0120172017-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/97155reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/971552026-06-02T12:44:21Z |
| dc.title.none.fl_str_mv |
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper |
| title |
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper |
| spellingShingle |
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper Mateescu, Bogdan 576.3 Extracellular vesicles Exosomes Non-coding RNA microRNA mRNA RNA sorting RNA binding proteins Quantification Biología celular (Biología) Biología molecular (Biología) 2407 Biología Celular 2415 Biología Molecular 2410.03 Citología Humana |
| title_short |
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper |
| title_full |
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper |
| title_fullStr |
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper |
| title_full_unstemmed |
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper |
| title_sort |
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper |
| dc.creator.none.fl_str_mv |
Mateescu, Bogdan Kowal, Emma Van Balkom, Bas Bartel, Sabine Bhattacharyya, Suvendra Buzás, Edit Buck, Amy Candia, Paola de Chow, Franklin Das, Saumya Driedonks, Tom Fernández Messina, Lola María Haderk, Franziska Hill, Andrew Jones, Jennifer Van Keuren‐Jensen, Kendall Lai, Charles Lässer, Cecilia di Liegro, Italia Lunavat, Taral Lorenowicz, Magdalena Maas, Sybren Mäger, Imre Mittelbrunn, Maria Momma, Stefan Mukherjee, Kamalika Nawaz, Muhammad Pegtel, Michiel Pfaffl, Michael Schiffelers, Raymond Tahara, Hidetoshi Théry, Clotilde Tosar, Juan Pablo Wauben, Marca Witwer, Kenneth Nolte‐'t Hoen, Esther |
| author |
Mateescu, Bogdan |
| author_facet |
Mateescu, Bogdan Kowal, Emma Van Balkom, Bas Bartel, Sabine Bhattacharyya, Suvendra Buzás, Edit Buck, Amy Candia, Paola de Chow, Franklin Das, Saumya Driedonks, Tom Fernández Messina, Lola María Haderk, Franziska Hill, Andrew Jones, Jennifer Van Keuren‐Jensen, Kendall Lai, Charles Lässer, Cecilia di Liegro, Italia Lunavat, Taral Lorenowicz, Magdalena Maas, Sybren Mäger, Imre Mittelbrunn, Maria Momma, Stefan Mukherjee, Kamalika Nawaz, Muhammad Pegtel, Michiel Pfaffl, Michael Schiffelers, Raymond Tahara, Hidetoshi Théry, Clotilde Tosar, Juan Pablo Wauben, Marca Witwer, Kenneth Nolte‐'t Hoen, Esther |
| author_role |
author |
| author2 |
Kowal, Emma Van Balkom, Bas Bartel, Sabine Bhattacharyya, Suvendra Buzás, Edit Buck, Amy Candia, Paola de Chow, Franklin Das, Saumya Driedonks, Tom Fernández Messina, Lola María Haderk, Franziska Hill, Andrew Jones, Jennifer Van Keuren‐Jensen, Kendall Lai, Charles Lässer, Cecilia di Liegro, Italia Lunavat, Taral Lorenowicz, Magdalena Maas, Sybren Mäger, Imre Mittelbrunn, Maria Momma, Stefan Mukherjee, Kamalika Nawaz, Muhammad Pegtel, Michiel Pfaffl, Michael Schiffelers, Raymond Tahara, Hidetoshi Théry, Clotilde Tosar, Juan Pablo Wauben, Marca Witwer, Kenneth Nolte‐'t Hoen, Esther |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidad Complutense de Madrid |
| dc.subject.none.fl_str_mv |
576.3 Extracellular vesicles Exosomes Non-coding RNA microRNA mRNA RNA sorting RNA binding proteins Quantification Biología celular (Biología) Biología molecular (Biología) 2407 Biología Celular 2415 Biología Molecular 2410.03 Citología Humana |
| topic |
576.3 Extracellular vesicles Exosomes Non-coding RNA microRNA mRNA RNA sorting RNA binding proteins Quantification Biología celular (Biología) Biología molecular (Biología) 2407 Biología Celular 2415 Biología Molecular 2410.03 Citología Humana |
| description |
The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells. Moreover, EV-RNA research faces various technical challenges, such as standardisation of EV isolation methods, optimisation of methodologies to isolate and characterise minute quantities of RNA found in EV, and development of approaches to demonstrate functional transfer of EV-RNA in vivo. These topics were discussed at the 2015 EV-RNA workshop of the International Society for Extracellular Vesicles. This position paper was written by the participants of the workshop not only to give an overview of the current state of knowledge in the field, but also to clarify that our incomplete knowledge – of the nature of EV(-RNA)s and of how to effectively and reliably study them – currently prohibits the implementation of gold standards in EV-RNA research. In addition, this paper creates awareness of possibilities and limitations of currently used strategies to investigate EV-RNA and calls for caution in interpretation of the obtained data. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 2017-01-01 2017 2017-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/20.500.14352/97155 |
| url |
https://hdl.handle.net/20.500.14352/97155 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Wiley |
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Wiley |
| dc.source.none.fl_str_mv |
reponame:Docta Complutense instname:Universidad Complutense de Madrid (UCM) |
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Universidad Complutense de Madrid (UCM) |
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Docta Complutense |
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Docta Complutense |
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1869406531040051200 |
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15,301603 |