The archaeal class Nitrososphaeria is a key component of the reproductive microbiome in sponges during gametogenesis

Sponge-associated microbes play fundamental roles in regulating their hosts' physiology, yet their contribution to sexual reproduction has been largely overlooked. Most studies have concentrated on the proportion of the microbiome transmitted from parents to offspring, providing little evidence...

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Detalles Bibliográficos
Autores: Turon, Marta, Koutsouveli, Vasiliki, Conejero, María, Taboada, Sergi, Verdes, Aida, Lorente-Sorolla, José María, Díez-Vives, Cristina, Riesgo, Ana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/415551
Acceso en línea:http://hdl.handle.net/10261/415551
https://api.elsevier.com/content/abstract/scopus_id/105008832828
Access Level:acceso abierto
Palabra clave:Porifera
Archaea
Gonochoristic
Immune system
Spermatogenesis
Transcriptome
Vitellogenesis
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oai_identifier_str oai:digital.csic.es:10261/415551
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network_name_str España
repository_id_str
dc.title.none.fl_str_mv The archaeal class Nitrososphaeria is a key component of the reproductive microbiome in sponges during gametogenesis
title The archaeal class Nitrososphaeria is a key component of the reproductive microbiome in sponges during gametogenesis
spellingShingle The archaeal class Nitrososphaeria is a key component of the reproductive microbiome in sponges during gametogenesis
Turon, Marta
Porifera
Archaea
Gonochoristic
Immune system
Spermatogenesis
Transcriptome
Vitellogenesis
title_short The archaeal class Nitrososphaeria is a key component of the reproductive microbiome in sponges during gametogenesis
title_full The archaeal class Nitrososphaeria is a key component of the reproductive microbiome in sponges during gametogenesis
title_fullStr The archaeal class Nitrososphaeria is a key component of the reproductive microbiome in sponges during gametogenesis
title_full_unstemmed The archaeal class Nitrososphaeria is a key component of the reproductive microbiome in sponges during gametogenesis
title_sort The archaeal class Nitrososphaeria is a key component of the reproductive microbiome in sponges during gametogenesis
dc.creator.none.fl_str_mv Turon, Marta
Koutsouveli, Vasiliki
Conejero, María
Taboada, Sergi
Verdes, Aida
Lorente-Sorolla, José María
Díez-Vives, Cristina
Riesgo, Ana
author Turon, Marta
author_facet Turon, Marta
Koutsouveli, Vasiliki
Conejero, María
Taboada, Sergi
Verdes, Aida
Lorente-Sorolla, José María
Díez-Vives, Cristina
Riesgo, Ana
author_role author
author2 Koutsouveli, Vasiliki
Conejero, María
Taboada, Sergi
Verdes, Aida
Lorente-Sorolla, José María
Díez-Vives, Cristina
Riesgo, Ana
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Fundación la Caixa
European Commission
Fundación Biodiversidad
Turon, Marta [0000-0002-3806-4937]
Taboada, Sergi [0000-0003-1669-1152]
Verdes, Aida [0000-0002-9193-9253]
Díez-Vives, Cristina [0000-0002-1772-7092]
Riesgo, Ana [0000-0002-7993-1523]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Porifera
Archaea
Gonochoristic
Immune system
Spermatogenesis
Transcriptome
Vitellogenesis
topic Porifera
Archaea
Gonochoristic
Immune system
Spermatogenesis
Transcriptome
Vitellogenesis
description Sponge-associated microbes play fundamental roles in regulating their hosts' physiology, yet their contribution to sexual reproduction has been largely overlooked. Most studies have concentrated on the proportion of the microbiome transmitted from parents to offspring, providing little evidence of the putative microbial role during gametogenesis in sponges. Here, we use 16S rRNA gene analysis to assess whether the microbial composition of five gonochoristic sponge species differs between reproductive and non-reproductive individuals and correlate these changes with their gametogenic stages. In sponges with mature oocytes, reproductive status did not influence either beta or alpha microbial diversity. However, in two of the studied species, Geodia macandrewii and Petrosia ficiformis, which presented oocytes at the previtellogenic stage, significant microbial composition changes were detected between reproductive and non-reproductive individuals. These disparities were primarily driven by differentially abundant taxa affiliated with the Nitrososphaeria archaeal class in both species. We speculate that the previtellogenic stages are more energetically demanding, leading to microbial changes due to the phagocytosis of microbes to meet nutritional demands during this period. Supporting our hypothesis, we observed significant transcriptomic differences in G. macandrewii, mainly associated with the immune system, indicating potential changes in the sponge's recognition system. Overall, we provide new insights into the possible roles of sponge microbiomes during reproductive periods, potentially uncovering critical interactions that support reproductive success.
publishDate 2025
dc.date.none.fl_str_mv 2025
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/415551
https://api.elsevier.com/content/abstract/scopus_id/105008832828
url http://hdl.handle.net/10261/415551
https://api.elsevier.com/content/abstract/scopus_id/105008832828
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/EC/H2020/101105716
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105769GB-I00
mBio
application/pdf
https://doi.org/10.1128/mbio.02019-24
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling The archaeal class Nitrososphaeria is a key component of the reproductive microbiome in sponges during gametogenesisTuron, MartaKoutsouveli, VasilikiConejero, MaríaTaboada, SergiVerdes, AidaLorente-Sorolla, José MaríaDíez-Vives, CristinaRiesgo, AnaPoriferaArchaeaGonochoristicImmune systemSpermatogenesisTranscriptomeVitellogenesisSponge-associated microbes play fundamental roles in regulating their hosts' physiology, yet their contribution to sexual reproduction has been largely overlooked. Most studies have concentrated on the proportion of the microbiome transmitted from parents to offspring, providing little evidence of the putative microbial role during gametogenesis in sponges. Here, we use 16S rRNA gene analysis to assess whether the microbial composition of five gonochoristic sponge species differs between reproductive and non-reproductive individuals and correlate these changes with their gametogenic stages. In sponges with mature oocytes, reproductive status did not influence either beta or alpha microbial diversity. However, in two of the studied species, Geodia macandrewii and Petrosia ficiformis, which presented oocytes at the previtellogenic stage, significant microbial composition changes were detected between reproductive and non-reproductive individuals. These disparities were primarily driven by differentially abundant taxa affiliated with the Nitrososphaeria archaeal class in both species. We speculate that the previtellogenic stages are more energetically demanding, leading to microbial changes due to the phagocytosis of microbes to meet nutritional demands during this period. Supporting our hypothesis, we observed significant transcriptomic differences in G. macandrewii, mainly associated with the immune system, indicating potential changes in the sponge's recognition system. Overall, we provide new insights into the possible roles of sponge microbiomes during reproductive periods, potentially uncovering critical interactions that support reproductive success.A.R. acknowledges funding from the Spanish Ministry of Science and Innovation, grants RYC2018-024247-I and PID2019-105769GB-I00, both funded by MCIN/AEI/10.13039/50110001103 and EI “FSE invierte en tu futuro.” C.D.-V. received the support of a fellowship from “la Caixa” Foundation (ID 100010434), with the fellowship code LCF/BQ/PI22/11910040. M.T. was supported by a JdC (Juan de la Cierva Formación, 2020) personal grant (FJC2020-043677-I) and MSCA Postdoctoral Fellowship (HORIZON-MSCA.2022-PF-01, Project 101105716). S.T. received funding from the grants PID2020-117115GA-100 of the Spanish Ministry of Science and Innovation and CNS2023-144572 and by the Ramón y Cajal grant RYC2021-03152-I, funded by the MCIN/AEI/10.13039/501100011033 and the European Union NextGenerationEU/PRTR. A.V. was supported by a European Union NextGenerationEU/PRTR grant (IJC2020-045256). Finally, part of this research was supported through the SponBIODIV project (A.R. and S.T.), a 2021-2022 BiodivProtect joint call for research proposals, under the Biodiversa+ Partnership co-funded by the European Commission, and with the funding organizations “Fundación Biodiversidad” and FORMAS.Peer reviewedAmerican Society for MicrobiologyMinisterio de Ciencia e Innovación (España)Fundación la CaixaEuropean CommissionFundación BiodiversidadTuron, Marta [0000-0002-3806-4937]Taboada, Sergi [0000-0003-1669-1152]Verdes, Aida [0000-0002-9193-9253]Díez-Vives, Cristina [0000-0002-1772-7092]Riesgo, Ana [0000-0002-7993-1523]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/415551https://api.elsevier.com/content/abstract/scopus_id/105008832828reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/101105716info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105769GB-I00mBioapplication/pdfhttps://doi.org/10.1128/mbio.02019-24Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4155512026-05-22T06:33:51Z
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