Ocular penetration of fluorometholone-loaded PEG-PLGA nanoparticles functionalized with cell-penetrating peptides
Aim: Development of fluorometholone-loaded PEG-PLGA nanoparticles (NPs) functionalized with cellpenetrating peptides for the treatment of ocular inflammatory disorders. Materials & methods: Synthesized polymers and peptides were used for elaboration of functionalized NPs, which were characterize...
| Authors: | , , , , , , , , , |
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| Format: | article |
| Status: | Versión aceptada para publicación |
| Publication Date: | 2019 |
| Country: | España |
| Institution: | Universidad de Barcelona |
| Repository: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/164191 |
| Online Access: | https://hdl.handle.net/2445/164191 |
| Access Level: | Open access |
| Keyword: | Farmacologia ocular Agents antiinflamatoris Sistemes d'alliberament de medicaments Nanopartícules Uveïtis Polímers Pèptids Ocular pharmacology Antiinflammatory agents Drug delivery systems Nanoparticles Uveitis Polymers Peptides |
| Summary: | Aim: Development of fluorometholone-loaded PEG-PLGA nanoparticles (NPs) functionalized with cellpenetrating peptides for the treatment of ocular inflammatory disorders. Materials & methods: Synthesized polymers and peptides were used for elaboration of functionalized NPs, which were characterized physicochemically. Cytotoxicity and ability to modulate the expression of proinflammatory cytokines were evaluated in vitro using human corneal epithelial cells (HCE-2). NP uptake was assayed in both in vitro and in vivo models. Results: NPs showed physicochemical characteristics suitable for ocular administration without evidence of cytotoxicity. TAT-NPs and G2-NPs were internalized and displayed anti-inflammatory activity in both HCE-2 cells and mouse eye. Conclusion: TAT-NPs and G2-NPs could be considered a novel strategy for the treatment of ocular inflammatory diseases of the anterior and posterior segment. |
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